Comparison of Immunofluorescence and Hemagglutination Inhibition Tests and Enzyme-linked Immunosorbent Assay for Detection of Serum Antibody in Rats Infected with Hemorrhagic Fever with Renal Syndrome Virus

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1986 Nov 1

PMID 2877007

Authors

Y Takahashi

Y Okuno

T Yamanouchi

N Takada

K Yamanishi

Affiliations

Soon will be listed here.

Abstract

Serum antibodies against the B-1 strain of hemorrhagic fever with renal syndrome (HFRS) virus in wild and experimental rats were investigated by the indirect immunofluorescent antibody (IF) test, the hemagglutination inhibition (HI) test, and an enzyme-linked immunosorbent assay (ELISA). In the newly developed ELISA, monoclonal antibodies to the B-1 strain were applied as coating antibody. In sera from wild rats, the results obtained by the ELISA agreed quite well with those obtained by the HI test, but some serum samples that gave a negative reaction in the HI test gave a positive reaction in the IF test, although their IF titers were very low. In serum samples from experimental rats that had been kept in an animal house infected with HFRS virus, a group with high titers and a group with low or negative titers were clearly differentiated by all the tests.

References

Lee H, Lee P, Johnson K . Isolation of the etiologic agent of Korean Hemorrhagic fever. J Infect Dis. 1978; 137(3):298-308. DOI: 10.1093/infdis/137.3.298. View

SVEDMYR A, Lee H, Berglund A, HOORN B, Nystrom K, Gajdusek D . Epidemic nephropathy in Scandinavia is related to Korean haemorrhagic fever. Lancet. 1979; 1(8107):100. DOI: 10.1016/s0140-6736(79)90083-7. View

Lee H, Lee P, Lahdevirta J . Aetiological relation between Korean haemorrhagic fever and nephropathia epidemica. Lancet. 1979; 1(8109):186-7. View

Lee H, Lee P, Tamura M, Tamura T, Okuno Y . Etiological relation between Korean hemorrhagic fever and epidemic hemorrhagic fever in Japan. Biken J. 1979; 22(2):41-5. View

BRUMMER-KORVENKONTIO M, Vaheri A, Hovi T, von Bonsdorff C, Vuorimies J, Manni T . Nephropathia epidemica: detection of antigen in bank voles and serologic diagnosis of human infection. J Infect Dis. 1980; 141(2):131-4. DOI: 10.1093/infdis/141.2.131. View

Lee P, SVEDMYR A, Gajdusek D, Gibbs Jr C, Nystrom K . Antigenic difference between European and East Asian viruses causing haemorrhagic fever with renal syndrome. Lancet. 1981; 2(8240):256-7. DOI: 10.1016/s0140-6736(81)90505-5. View

Tamura M . OCCURRENCE OF EPIDEMIC HEMORRHAGIC FEVER IN OSAKA CITY: FIRST CASES FOUND IN JAPAN WITH CHARACTERISTIC FEATURE OF MARKED PROTEINURIA. Biken J. 1964; 7:79-94. View

Yamanishi K, Dantas J, Takahashi M, Yamanouchi T, Domae K, Kawamata J . Isolation of hemorrhagic fever with renal syndrome (HFRS) virus from a tumor specimen in a rat. Biken J. 1983; 26(4):155-60. View

Yamanishi K, Dantas Jr J, Takahashi M, Yamanouchi T, Domae K, Takahashi Y . Antigenic differences between two viruses, isolated in Japan and Korea, that cause hemorrhagic fever with renal syndrome. J Virol. 1984; 52(1):231-7. PMC: 254510. DOI: 10.1128/JVI.52.1.231-237.1984. View

10.

Yanagihara R, SVEDMYR A, Amyx H, Lee P, Goldgaber D, Gajdusek D . Isolation and propagation of nephropathia epidemica virus in bank voles. Scand J Infect Dis. 1984; 16(3):225-8. DOI: 10.3109/00365548409070393. View

11.

Okuno Y, Yamanishi K, Takahashi Y, Tanishita O, Nagai T, Dantas Jr J . Haemagglutination-inhibition test for haemorrhagic fever with renal syndrome using virus antigen prepared from infected tissue culture fluid. J Gen Virol. 1986; 67 ( Pt 1):149-56. DOI: 10.1099/0022-1317-67-1-149. View

12.

Dantas Jr J, Okuno Y, Asada H, Tamura M, Takahashi M, Tanishita O . Characterization of glycoproteins of viruses causing hemorrhagic fever with renal syndrome (HFRS) using monoclonal antibodies. Virology. 1986; 151(2):379-84. DOI: 10.1016/0042-6822(86)90058-9. View

13.

Lee P, Amyx H, Gajdusek D, Yanagihara R, Goldgaber D, Gibbs Jr C . New hemorrhagic fever with renal syndrome-related virus in rodents in the United States. Lancet. 1982; 2(8312):1405. View