Echinacoside's Nigrostriatal Dopaminergic Protection Against 6-OHDA-Induced Endoplasmic Reticulum Stress Through Reducing the Accumulation of Seipin

Overview

Journal J Cell Mol Med

Specialties Cell Biology
Molecular Biology

Date 2017 Aug 3

PMID 28767194

Citations 22

Authors

Yajie Zhang

Hongyan Long

Fuqiong Zhou

Weina Zhu

Jie Ruan

Yang Zhao

Yan Lu

Affiliations

Soon will be listed here.

Abstract

Parkinson's disease (PD) is one of the most common neurodegenerative diseases. Recent epidemiological studies suggest that echinacoside (ECH), a phenylethanoid glycoside found in Cistanche deserticola, has a protective effect against the development of PD. However, the detailed mechanisms of how ECH suppresses neuronal death have not been fully elucidated. In this study, we confirmed that ECH protects nigrostriatal neurons against 6-hydroxydopamine (6-OHDA)-induced endoplasmic reticulum stress (ERS) in vivo and in vitro. ECH rescued cell viability in damaged cells and decreased 6-OHDA-induced reactive oxygen species accumulation in vitro. It also rescued tyrosine hydroxylase and dopamine transporter expression in the striatum, and decreased α-synuclein aggregation following 6-OHDA treatment in vivo. The validated mechanism of ECH activity was the reduction in the 6-OHDA-induced accumulation of seipin (Berardinelli-Seip congenital lipodystrophy 2). Seipin has been shown to be a key molecule related to motor neuron disease and was tightly associated with ERS in a series of in vivo studies. ECH attenuated seipinopathy by promoting seipin degradation via ubiquitination. ERS was relieved by ECH through the Grp94/Bip-ATF4-CHOP signal pathway.

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