Abnormalities in Ileal Stem, Neurogenin 3, and Enteroendocrine Cells in Patients with Irritable Bowel Syndrome

Overview

Journal BMC Gastroenterol

Publisher Biomed Central

Specialty Gastroenterology

Date 2017 Aug 3

PMID 28764761

Citations 10

Authors

Magdy El-Salhy

Odd Helge Gilja

Affiliations

Soon will be listed here.

Abstract

Background: This study examined whether the densities of stem- and enteroendocrine cell progenitors are abnormal in the ileum of patients with irritable bowel syndrome (IBS), and whether any abnormalities in ileal enteroendocrine cells are correlated with abnormalities in stem cells and enteroendocrine cell progenitors.

Methods: One hundred and one IBS patients covering all IBS subtypes were recruited, and 39 non-IBS subjects were included as a control group. The patients and controls underwent standard colonoscopies, during which biopsy specimens were obtained from the ileum. The biopsy specimens were stained with hematoxylin-eosin and immunostained for Musashi-1 (Msi-1), neurogenin 3 (NEUROG3), chromogranin A (CgA), serotonin, peptide YY (PYY), oxyntomodulin (enteroglucagon), pancreatic polypeptide, and somatostatin. The immunoreactive cells were quantified by computerized image analysis.

Results: The densities of Msi-1, NEUROG3, CgA, and serotonin cells were reduced in all IBS patients and in patients with diarrhea-predominant IBS (IBS-D), mixed-diarrhea-and-constipation IBS (IBS-M), and constipation-predominant (IBS-C) relative to the control subjects. While the PYY cell density was increased in IBS-C relative to controls, it did not differ between control subjects and IBS-D and IBS-M patients. The densities of Msi-1 and NEUROG3 cells were strongly correlated with that of CgA cells.

Conclusions: The abnormalities in the ileal enteroendocrine cells appear to be caused by two mechanisms: (1) decreases in the clonogenic activity of the stem cells and in the endocrine-cell progenitors differentiating into enteroendocrine cells, and (2) switching on the expression of PYY and switching off the expression of certain other hormones in other types of the enteroendocrine cells.

Citing Articles

Fundamental Neurochemistry Review: The role of enteroendocrine cells in visceral pain.

Londregan A, Alexander T, Covarrubias M, Waldman S J Neurochem. 2023; 167(6):719-732.

PMID: 38037432 PMC: 10917140. DOI: 10.1111/jnc.16022.

Enteroendocrine cell regulation of the gut-brain axis.

Barton J, Londregan A, Alexander T, Entezari A, Covarrubias M, Waldman S Front Neurosci. 2023; 17:1272955.

PMID: 38027512 PMC: 10662325. DOI: 10.3389/fnins.2023.1272955.

The Effects of Fecal Microbiota Transplantation on the Symptoms and the Duodenal Neurogenin 3, Musashi 1, and Enteroendocrine Cells in Patients With Diarrhea-Predominant Irritable Bowel Syndrome.

Mazzawi T, El-Salhy M, Lied G, Hausken T Front Cell Infect Microbiol. 2021; 11:524851.

PMID: 34055657 PMC: 8149964. DOI: 10.3389/fcimb.2021.524851.

Molecular Mechanisms of Microbiota-Mediated Pathology in Irritable Bowel Syndrome.

Mishima Y, Ishihara S Int J Mol Sci. 2020; 21(22).

PMID: 33212919 PMC: 7698457. DOI: 10.3390/ijms21228664.

Density of Musashi‑1‑positive stem cells in the stomach of patients with irritable bowel syndrome.

El-Salhy M, Hausken T, Hatlebakk J Mol Med Rep. 2020; 22(4):3135-3140.

PMID: 32945509 PMC: 7453583. DOI: 10.3892/mmr.2020.11412.

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