Functional and Promoter Analysis of ChiIV3, a Chitinase of Pepper Plant, in Response to Phytophthora Capsici Infection

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2017 Aug 2

PMID 28763001

Citations 17

Authors

Zhiqin Liu

Lanping Shi

Sheng Yang

Youquan Lin

Yahong Weng

Xia Li

Ansar Hussain

Ali Noman

Shuilin He

Affiliations

Soon will be listed here.

Abstract

Despite the involvement of many members of the chitinase family in plant immunity, the precise functions of the majority of the members remain poorly understood. Herein, the gene in encoding a chitinase protein containing a chitin binding domain and targeting to the plasma membrane was found to be induced by inoculation (PCI) and applied chitin treatment. Besides its direct inhibitory effect on growth of (), ChiIV3 was also found by virus-induced gene silencing (VIGS) and transient overexpression (TOE) in pepper plants to act as a positive regulator of plant cell death and in triggering defense signaling and upregulation of (pathogenesis related) genes against PCI. A 5' deletion assay revealed that was found to be sufficient for response to PCI. Furthermore, a mutation assay indicated that W-box in was noted to be the PCI-responsible element. These results collectively suggest that ChiIV3 acts as a likely antifungal protein and as a receptor for unidentified chitin in planta to trigger cell death and defense signaling against PCI.

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