In Vitro Bioactivity of One- and Two-Dimensional Nanoparticle-Incorporated Bone Tissue Engineering Scaffolds

Overview

Journal Tissue Eng Part A

Specialties Anatomy & Histology
Biomedical Engineering
Biotechnology

Date 2017 Aug 2

PMID 28762866

Citations 4

Authors

Jason T Rashkow

Gaurav Lalwani

Balaji Sitharaman

Affiliations

Soon will be listed here.

Abstract

This study investigates the effect of incorporation of one- or two-dimensional nanoparticles with distinct composition and morphology on the bioactivity of biodegradable, biocompatible polymer matrices. 0.2 wt% multiwalled carbon nanotubes, multiwalled graphene nanoribbons, graphene oxide nanoplatelets (GONPs), molybdenum disulfide nanoplatelets (MSNPs), or tungsten disulfide nanotubes (WSNTs) were uniformly dispersed in poly(lactic-co-glycolic acid) (PLGA) polymer. PLGA or nanoparticle-incorporated PLGA were then incubated with simulated body fluid (SBF) under physiological conditions for 1, 3, 7, or 14 days. Apatite collection on control and incorporated scaffolds was assessed. All groups showed apatite precipitate on the surface after 1 day of SBF incubation. After 14 days of SBF incubation, scaffolds incorporated with GONPs, MSNPs, or WSNTs showed significantly higher phosphate accumulation compared to PLGA scaffolds. Scaffolds incorporated with GONPs, MSNPs, or WSNTs should be studied in vivo to further investigate potential bioactivity, leading to enhanced integration and tissue repair at the bone-implant interface.

Citing Articles

Effect of Atmospheric Pressure Plasma Jet Treatments on Magnesium Phosphate Cements: Performance, Characterization, and Applications.

Gelli R, Tonelli M, Ridi F, Terefinko D, Dzimitrowicz A, Pohl P ACS Biomater Sci Eng. 2023; 9(12):6632-6643.

PMID: 37982239 PMC: 10716815. DOI: 10.1021/acsbiomaterials.3c00817.

The Role of Tantalum Nanoparticles in Bone Regeneration Involves the BMP2/Smad4/Runx2 Signaling Pathway.

Zhang G, Liu W, Wang R, Zhang Y, Chen L, Chen A Int J Nanomedicine. 2020; 15:2419-2435.

PMID: 32368035 PMC: 7174976. DOI: 10.2147/IJN.S245174.

Use of nanoparticles in skeletal tissue regeneration and engineering.

Filippi M, Born G, Felder-Flesch D, Scherberich A Histol Histopathol. 2019; 35(4):331-350.

PMID: 31721139 DOI: 10.14670/HH-18-184.

Impact of functional inorganic nanotubes f-INTs-WS on hemolysis, platelet function and coagulation.

Laloy J, Haguet H, Alpan L, Raichman D, Dogne J, Lellouche J Nano Converg. 2018; 5(1):31.

PMID: 30467733 PMC: 6206311. DOI: 10.1186/s40580-018-0162-1.

References

Ngiam M, Liao S, Patil A, Cheng Z, Chan C, Ramakrishna S . The fabrication of nano-hydroxyapatite on PLGA and PLGA/collagen nanofibrous composite scaffolds and their effects in osteoblastic behavior for bone tissue engineering. Bone. 2009; 45(1):4-16. DOI: 10.1016/j.bone.2009.03.674. View

Gerhardt L, Jell G, Boccaccini A . Titanium dioxide (TiO(2)) nanoparticles filled poly(D,L lactid acid) (PDLLA) matrix composites for bone tissue engineering. J Mater Sci Mater Med. 2007; 18(7):1287-98. DOI: 10.1007/s10856-006-0062-5. View

Hong Z, Reis R, Mano J . Preparation and in vitro characterization of scaffolds of poly(L-lactic acid) containing bioactive glass ceramic nanoparticles. Acta Biomater. 2008; 4(5):1297-306. DOI: 10.1016/j.actbio.2008.03.007. View

Rashkow J, Talukdar Y, Lalwani G, Sitharaman B . Interactions of 1D- and 2D-layered inorganic nanoparticles with fibroblasts and human mesenchymal stem cells. Nanomedicine (Lond). 2015; 10(11):1693-706. PMC: 4576357. DOI: 10.2217/nnm.15.35. View

Kyriazis V, Tzaphlidou M . Skeletal calcium/phosphorus ratio measuring techniques and results. I. Microscopy and microtomography. ScientificWorldJournal. 2004; 4:1027-34. PMC: 5956472. DOI: 10.1100/tsw.2004.200. View

Boccaccini A, Blaker J . Bioactive composite materials for tissue engineering scaffolds. Expert Rev Med Devices. 2005; 2(3):303-17. DOI: 10.1586/17434440.2.3.303. View

Sitharaman B, Shi X, Tran L, Spicer P, Rusakova I, Wilson L . Injectable in situ cross-linkable nanocomposites of biodegradable polymers and carbon nanostructures for bone tissue engineering. J Biomater Sci Polym Ed. 2007; 18(6):655-71. DOI: 10.1163/156856207781034133. View

Zawadzak E, Bil M, Ryszkowska J, Nazhat S, Cho J, Bretcanu O . Polyurethane foams electrophoretically coated with carbon nanotubes for tissue engineering scaffolds. Biomed Mater. 2008; 4(1):015008. DOI: 10.1088/1748-6041/4/1/015008. View

Dinescu S, Ionita M, Pandele A, Galateanu B, Iovu H, Ardelean A . In vitro cytocompatibility evaluation of chitosan/graphene oxide 3D scaffold composites designed for bone tissue engineering. Biomed Mater Eng. 2014; 24(6):2249-56. DOI: 10.3233/BME-141037. View

10.

Smith I, Liu X, Smith L, Ma P . Nanostructured polymer scaffolds for tissue engineering and regenerative medicine. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010; 1(2):226-36. PMC: 2800311. DOI: 10.1002/wnan.26. View

11.

Choi S, Yu X, Jongpaiboonkit L, Hollister S, Murphy W . Inorganic coatings for optimized non-viral transfection of stem cells. Sci Rep. 2013; 3:1567. PMC: 3610100. DOI: 10.1038/srep01567. View

12.

Makadia H, Siegel S . Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery Carrier. Polymers (Basel). 2012; 3(3):1377-1397. PMC: 3347861. DOI: 10.3390/polym3031377. View

13.

Bohner M, Lemaitre J . Can bioactivity be tested in vitro with SBF solution?. Biomaterials. 2009; 30(12):2175-9. DOI: 10.1016/j.biomaterials.2009.01.008. View

14.

Lalwani G, Henslee A, Farshid B, Parmar P, Lin L, Qin Y . Tungsten disulfide nanotubes reinforced biodegradable polymers for bone tissue engineering. Acta Biomater. 2013; 9(9):8365-73. PMC: 3732565. DOI: 10.1016/j.actbio.2013.05.018. View

15.

Blokhuis T, Arts J . Bioactive and osteoinductive bone graft substitutes: definitions, facts and myths. Injury. 2011; 42 Suppl 2:S26-9. DOI: 10.1016/j.injury.2011.06.010. View

16.

Kokubo T, Takadama H . How useful is SBF in predicting in vivo bone bioactivity?. Biomaterials. 2006; 27(15):2907-15. DOI: 10.1016/j.biomaterials.2006.01.017. View

17.

Mikael P, Amini A, Basu J, Arellano-Jimenez M, Laurencin C, Sanders M . Functionalized carbon nanotube reinforced scaffolds for bone regenerative engineering: fabrication, in vitro and in vivo evaluation. Biomed Mater. 2014; 9(3):035001. DOI: 10.1088/1748-6041/9/3/035001. View

18.

Talukdar Y, Rashkow J, Lalwani G, Kanakia S, Sitharaman B . The effects of graphene nanostructures on mesenchymal stem cells. Biomaterials. 2014; 35(18):4863-4877. PMC: 3995421. DOI: 10.1016/j.biomaterials.2014.02.054. View

19.

Morgan B, Artiss J, ZAK B . Calcium determination in serum with stable alkaline Arsenazo III and triglyceride clearing. Clin Chem. 1993; 39(8):1608-12. View

20.

Chen Q, Thompson I, Boccaccini A . 45S5 Bioglass-derived glass-ceramic scaffolds for bone tissue engineering. Biomaterials. 2005; 27(11):2414-25. DOI: 10.1016/j.biomaterials.2005.11.025. View