M-RNA Gene Expression of INF-Γ and IL-10 During Intestinal Phase of After Myrrh and Albendazole Treatment

Overview

Journal Iran J Parasitol

Publisher Tehran University of Medical Sciences

Specialty Parasitology

Date 2017 Aug 2

PMID 28761478

Citations 1

Authors

Hanaa Y Bakir

Rasha Ah Attia

Abeer E Mahmoud

Zedan Ibraheim

Affiliations

Soon will be listed here.

Abstract

Background: The protective response developed against infection provokes immune and inflammatory responses mediated by cytokines released from T helper cells. We aimed to evaluate the effect of albendazole or myrrh on the expression of IFN-γ and IL-10 in BALB/c mice infected with .

Methods: This study was done at the Animal House of Faculty of Medicine, Assiut University (Assiut, Egypt) from April to December 2015. Mice were infected with 300 larvae and treated with albendazole (50 mg/kg per day) or myrrh (500 mg/kg per day) for 3 consecutive days post-infection (pi). The expression of INF-γ and IL-10 was detected in the intestinal tissue by reverse transcription (RT) PCR.

Results: The expression of IFN-γ in mice treated with albendazole and myrrh was detected on days 3 and 15 pi respectively. In the control group, it was found on days 5, 10, 15 and 20 pi with the highest expression on day 15 pi. The expression of IL-10 was detected on days 3, 20 pi in the albendazole and myrrh treated groups, respectively. In the control group, IL-10 expression appeared on days 5 and day 20 pi.

Conclusion: The target of albendazole and myrrh on the profile of IFN-γ and IL-10 on these cytokines were encouraging to reinforce their therapeutic use against trichinellosis.

Citing Articles

Therapeutic effects of myrrh extract and myrrh-based silver nanoparticles on -infected mice: parasitological, histopathological, and immunological (IFN-γ, IL-10, and MMP-9) investigations.

Abd-ELrahman S, Dyab A, Mahmoud A, Mohamed S, Fouad A, Gareh A Front Vet Sci. 2024; 11:1433964.

PMID: 39421828 PMC: 11483346. DOI: 10.3389/fvets.2024.1433964.

References

Blish C, Sangare L, Herrin B, Richardson B, John-Stewart G, Walson J . Changes in plasma cytokines after treatment of ascaris lumbricoides infection in individuals with HIV-1 infection. J Infect Dis. 2010; 201(12):1816-21. PMC: 2946624. DOI: 10.1086/652784. View

Repetto G, Peso A, Zurita J . Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nat Protoc. 2008; 3(7):1125-31. DOI: 10.1038/nprot.2008.75. View

Chen K, Lai S . Biochemical and pathological evaluation of albendazole/thalidomide co-therapy against eosinophilic meningitis or meningoencephalitis induced by Angiostrongylus cantonensis. J Antimicrob Chemother. 2007; 59(2):264-76. DOI: 10.1093/jac/dkl492. View

Despommier D . Trichinella spiralis and the concept of niche. J Parasitol. 1993; 79(4):472-82. View

Chung M, Joo K, Quan F, Kwon H, Cho S . Efficacy of flubendazole and albendazole against Trichinella spiralis in mice. Parasite. 2001; 8(2 Suppl):S195-8. DOI: 10.1051/parasite/200108s2195. View

Siriyasatien P, Yingyourd P, Nuchprayoon S . Efficacy of albendazole against early and late stage of Trichinella spiralis infection in mice. J Med Assoc Thai. 2003; 86 Suppl 2:S257-62. View

Diao Z, Chen X, Yin C, Wang J, Qi H, Ji A . Angiostrongylus cantonensis: effect of combination therapy with albendazole and dexamethasone on Th cytokine gene expression in PBMC from patients with eosinophilic meningitis. Exp Parasitol. 2009; 123(1):1-5. DOI: 10.1016/j.exppara.2009.04.016. View

Attia R, Mahmoud A, Farrag H, Makboul R, Embarek Mohamed M, Ibraheim Z . Effect of myrrh and thyme on Trichinella spiralis enteral and parenteral phases with inducible nitric oxide expression in mice. Mem Inst Oswaldo Cruz. 2015; 110(8):1035-41. PMC: 4708024. DOI: 10.1590/0074-02760150295. View

Kim S, Park M, Yu H . Toll-Like Receptor Gene Expression during Trichinella spiralis Infection. Korean J Parasitol. 2015; 53(4):431-8. PMC: 4566501. DOI: 10.3347/kjp.2015.53.4.431. View

10.

Dozois C, Oswald E, Gautier N, Serthelon J, Fairbrother J, Oswald I . A reverse transcription-polymerase chain reaction method to analyze porcine cytokine gene expression. Vet Immunol Immunopathol. 1998; 58(3-4):287-300. DOI: 10.1016/s0165-2427(97)00039-1. View

11.

Cooper P, Moncayo A, Guadalupe I, Benitez S, Vaca M, Chico M . Repeated treatments with albendazole enhance Th2 responses to Ascaris Lumbricoides, but not to aeroallergens, in children from rural communities in the Tropics. J Infect Dis. 2008; 198(8):1237-42. PMC: 2635537. DOI: 10.1086/591945. View

12.

Li C, Seth R, Gray T, Bayston R, Mahida Y, Wakelin D . Production of proinflammatory cytokines and inflammatory mediators in human intestinal epithelial cells after invasion by Trichinella spiralis. Infect Immun. 1998; 66(5):2200-6. PMC: 108182. DOI: 10.1128/IAI.66.5.2200-2206.1998. View

13.

Hussaini S, Hassanali H . Limulus amoebocyte lysate assay of endotoxin: a method for visual detection of the positive gel reaction. J Med Microbiol. 1987; 24(1):89-90. DOI: 10.1099/00222615-24-1-89. View

14.

Dolara P, Corte B, Ghelardini C, Pugliese A, Cerbai E, Menichetti S . Local anaesthetic, antibacterial and antifungal properties of sesquiterpenes from myrrh. Planta Med. 2000; 66(4):356-8. DOI: 10.1055/s-2000-8532. View

15.

Shalaby M, Moghazy F, Shalaby H, Nasr S . Effect of methanolic extract of Balanites aegyptiaca fruits on enteral and parenteral stages of Trichinella spiralis in rats. Parasitol Res. 2010; 107(1):17-25. DOI: 10.1007/s00436-010-1827-9. View

16.

Wojtkowiak-Giera A, Wandurska-Nowak E, Michalak M, Derda M, Lopaciuch J . Trichinellosis in mice: effect of albendazole on the glutathione transferase in the intestines. Folia Parasitol (Praha). 2013; 59(4):311-4. DOI: 10.14411/fp.2012.044. View

17.

Kang S, Cho M, Park M, Kim D, Hong Y, Lee Y . Alteration of helper T-cell related cytokine production in splenocytes during Trichinella spiralis infection. Vet Parasitol. 2012; 186(3-4):319-27. DOI: 10.1016/j.vetpar.2011.12.002. View

18.

Bliss S, Bliss S, Beiting D, Alcaraz A, Appleton J . IL-10 regulates movement of intestinally derived CD4+ T cells to the liver. J Immunol. 2007; 178(12):7974-83. DOI: 10.4049/jimmunol.178.12.7974. View

19.

Ahmad A, Raish M, Ganaie M, Ahmad S, Mohsin K, Al-Jenoobi F . Hepatoprotective effect of Commiphora myrrha against d-GalN/LPS-induced hepatic injury in a rat model through attenuation of pro inflammatory cytokines and related genes. Pharm Biol. 2015; 53(12):1759-67. DOI: 10.3109/13880209.2015.1005754. View

20.

M A Basyoni M, El-Sabaa A . Therapeutic potential of myrrh and ivermectin against experimental Trichinella spiralis infection in mice. Korean J Parasitol. 2013; 51(3):297-304. PMC: 3712103. DOI: 10.3347/kjp.2013.51.3.297. View