Mobile Phone Radiofrequency Exposure Has No Effect on DNA Double Strand Breaks (DSB) in Human Lymphocytes

Overview

Journal Ann Transl Med

Publisher AME Publishing Company

Date 2017 Aug 1

PMID 28758098

Citations 6

Authors

Elisa Danese

Giuseppe Lippi

Ruggero Buonocore

Marco Benati

Chiara Bovo

Chiara Bonaguri

Gian Luca Salvagno

Giorgio Brocco

Dirk Roggenbuck

Martina Montagnana

Affiliations

Soon will be listed here.

Abstract

Background: The use of mobile phones has been associated with an increased risk of developing certain type of cancer, especially in long term users. Therefore, this study was aimed to investigate the potential genotoxic effect of mobile phone radiofrequency exposure on human peripheral blood mononuclear cells

Methods: The study population consisted in 14 healthy volunteers. After collection of two whole blood samples, the former was placed in a plastic rack, 1 cm from the chassis of a commercial mobile phone (900 MHz carrier frequency), which was activated by a 30-min call. The second blood sample was instead maintained far from mobile phones or other RF sources. The influence of mobile phone RF on DNA integrity was assessed by analyzing γ-H2AX foci in lymphocytes using immunofluorescence staining kit on AKLIDES.

Results: No measure of γ-H2AX foci was significantly influenced by mobile phone RF exposure, nor mobile phone exposure was associated with significant risk of genetic damages (odds ratio comprised between 0.27 and 1.00).

Conclusions: The results of this experimental study demonstrate that exposure of human lymphocytes to a conventional 900 MHz RF emitted by a commercial mobile phone for 30 min does not significantly impact DNA integrity.

Citing Articles

Study of genotoxic and cytotoxic effects induced in human fibroblasts by exposure to pulsed and continuous 1.6 GHz radiofrequency.

Massaro L, De Sanctis S, Franchini V, Regalbuto E, Alfano G, Focaccetti C Front Public Health. 2024; 12:1419525.

PMID: 39145180 PMC: 11323689. DOI: 10.3389/fpubh.2024.1419525.

Human Fibroblasts In Vitro Exposed to 2.45 GHz Continuous and Pulsed Wave Signals: Evaluation of Biological Effects with a Multimethodological Approach.

Regalbuto E, Anselmo A, De Sanctis S, Franchini V, Lista F, Benvenuto M Int J Mol Sci. 2020; 21(19).

PMID: 32992895 PMC: 7584027. DOI: 10.3390/ijms21197069.

Microwaves from mobile phone induce reactive oxygen species but not DNA damage, preleukemic fusion genes and apoptosis in hematopoietic stem/progenitor cells.

Durdik M, Kosik P, Markova E, Somsedikova A, Gajdosechova B, Nikitina E Sci Rep. 2019; 9(1):16182.

PMID: 31700008 PMC: 6838175. DOI: 10.1038/s41598-019-52389-x.

Electromagnetic Fields of Mobile Phone Jammer Exposure on Blood Factors in Rats.

Shojaeifard M, Jarideh S, Owjfard M, Nematollahii S, Talaei-Khozani T, Malekzadeh M J Biomed Phys Eng. 2018; 8(4):403-408.

PMID: 30568930 PMC: 6280113.

Comment on "Mobile phone radiofrequency exposure has no effect on DNA double strand breaks (DSB) in human lymphocytes".

Mortazavi S Ann Transl Med. 2017; 5(21):441.

PMID: 29201892 PMC: 5690963. DOI: 10.21037/atm.2017.08.39.

References

Belyaev I, Eriksson S, Nygren J, Torudd J, Harms-Ringdahl M . Effects of ethidium bromide on DNA loop organisation in human lymphocytes measured by anomalous viscosity time dependence and single cell gel electrophoresis. Biochim Biophys Acta. 1999; 1428(2-3):348-56. DOI: 10.1016/s0304-4165(99)00076-8. View

Verschaeve L, Heikkinen P, Verheyen G, Gorp U, Boonen F, Vander Plaetse F . Investigation of co-genotoxic effects of radiofrequency electromagnetic fields in vivo. Radiat Res. 2006; 165(5):598-607. DOI: 10.1667/RR3559.1. View

Tepe Cam S, Seyhan N . Single-strand DNA breaks in human hair root cells exposed to mobile phone radiation. Int J Radiat Biol. 2012; 88(5):420-4. DOI: 10.3109/09553002.2012.666005. View

Gong X, Wu J, Mao Y, Zhou L . [Long-term use of mobile phone and its association with glioma: a systematic review and meta-analysis]. Zhonghua Yi Xue Za Zhi. 2015; 94(39):3102-6. View

Ivashkevich A, Martin O, Smith A, Redon C, Bonner W, Martin R . γH2AX foci as a measure of DNA damage: a computational approach to automatic analysis. Mutat Res. 2011; 711(1-2):49-60. PMC: 3101310. DOI: 10.1016/j.mrfmmm.2010.12.015. View

Rogakou E, Boon C, Redon C, Bonner W . Megabase chromatin domains involved in DNA double-strand breaks in vivo. J Cell Biol. 1999; 146(5):905-16. PMC: 2169482. DOI: 10.1083/jcb.146.5.905. View

Runge R, Hiemann R, Wendisch M, Kasten-Pisula U, Storch K, Zophel K . Fully automated interpretation of ionizing radiation-induced γH2AX foci by the novel pattern recognition system AKLIDES®. Int J Radiat Biol. 2012; 88(5):439-47. DOI: 10.3109/09553002.2012.658468. View

Hou Q, Wang M, Wu S, Ma X, An G, Liu H . Oxidative changes and apoptosis induced by 1800-MHz electromagnetic radiation in NIH/3T3 cells. Electromagn Biol Med. 2014; 34(1):85-92. DOI: 10.3109/15368378.2014.900507. View

Lai H, Singh N . Acute low-intensity microwave exposure increases DNA single-strand breaks in rat brain cells. Bioelectromagnetics. 1995; 16(3):207-10. DOI: 10.1002/bem.2250160309. View

10.

Verschaeve L, Juutilainen J, Lagroye I, Miyakoshi J, Saunders R, de Seze R . In vitro and in vivo genotoxicity of radiofrequency fields. Mutat Res. 2010; 705(3):252-68. DOI: 10.1016/j.mrrev.2010.10.001. View

11.

Lagorio S, Roosli M . Mobile phone use and risk of intracranial tumors: a consistency analysis. Bioelectromagnetics. 2013; 35(2):79-90. DOI: 10.1002/bem.21829. View

12.

Lai H, Singh N . Single- and double-strand DNA breaks in rat brain cells after acute exposure to radiofrequency electromagnetic radiation. Int J Radiat Biol. 1996; 69(4):513-21. DOI: 10.1080/095530096145814. View

13.

Schwab S, Brand M, Schlude I, Wuest W, Meier-Meitinger M, Distel L . X-ray induced formation of γ-H2AX foci after full-field digital mammography and digital breast-tomosynthesis. PLoS One. 2013; 8(7):e70660. PMC: 3723730. DOI: 10.1371/journal.pone.0070660. View

14.

Belyaev I, Markova E, Hillert L, Malmgren L, Persson B . Microwaves from UMTS/GSM mobile phones induce long-lasting inhibition of 53BP1/gamma-H2AX DNA repair foci in human lymphocytes. Bioelectromagnetics. 2008; 30(2):129-41. DOI: 10.1002/bem.20445. View

15.

Agarwal A, Deepinder F, Sharma R, Ranga G, Li J . Effect of cell phone usage on semen analysis in men attending infertility clinic: an observational study. Fertil Steril. 2007; 89(1):124-8. DOI: 10.1016/j.fertnstert.2007.01.166. View

16.

Stronati L, Testa A, Moquet J, Edwards A, Cordelli E, Villani P . 935 MHz cellular phone radiation. An in vitro study of genotoxicity in human lymphocytes. Int J Radiat Biol. 2006; 82(5):339-46. DOI: 10.1080/09553000600739173. View

17.

Siddiqui M, Francois M, Fenech M, Leifert W . Persistent γH2AX: A promising molecular marker of DNA damage and aging. Mutat Res Rev Mutat Res. 2015; 766:1-19. DOI: 10.1016/j.mrrev.2015.07.001. View

18.

Rothkamm K, Balroop S, Shekhdar J, Fernie P, Goh V . Leukocyte DNA damage after multi-detector row CT: a quantitative biomarker of low-level radiation exposure. Radiology. 2006; 242(1):244-51. DOI: 10.1148/radiol.2421060171. View

19.

Marinelli F, La Sala D, Cicciotti G, Cattini L, Trimarchi C, Putti S . Exposure to 900 MHz electromagnetic field induces an unbalance between pro-apoptotic and pro-survival signals in T-lymphoblastoid leukemia CCRF-CEM cells. J Cell Physiol. 2003; 198(2):324-32. DOI: 10.1002/jcp.10425. View

20.

Willitzki A, Hiemann R, Peters V, Sack U, Schierack P, Rodiger S . New platform technology for comprehensive serological diagnostics of autoimmune diseases. Clin Dev Immunol. 2013; 2012:284740. PMC: 3536031. DOI: 10.1155/2012/284740. View