Aurora-B Kinase Pathway Controls the Lateral to End-on Conversion of Kinetochore-microtubule Attachments in Human Cells

Overview

Journal Nat Commun

Specialty Biology

Date 2017 Jul 29

PMID 28751710

Citations 43

Authors

Roshan L Shrestha

Duccio Conti

Naoka Tamura

Dominique Braun

Revathy A Ramalingam

Konstanty Cieslinski

Jonas Ries

Viji M Draviam

Affiliations

Soon will be listed here.

Abstract

Human chromosomes are captured along microtubule walls (lateral attachment) and then tethered to microtubule-ends (end-on attachment) through a multi-step end-on conversion process. Upstream regulators that orchestrate this remarkable change in the plane of kinetochore-microtubule attachment in human cells are not known. By tracking kinetochore movements and using kinetochore markers specific to attachment status, we reveal a spatially defined role for Aurora-B kinase in retarding the end-on conversion process. To understand how Aurora-B activity is counteracted, we compare the roles of two outer-kinetochore bound phosphatases and find that BubR1-associated PP2A, unlike KNL1-associated PP1, plays a significant role in end-on conversion. Finally, we uncover a novel role for Aurora-B regulated Astrin-SKAP complex in ensuring the correct plane of kinetochore-microtubule attachment. Thus, we identify Aurora-B as a key upstream regulator of end-on conversion in human cells and establish a late role for Astrin-SKAP complex in the end-on conversion process.Human chromosomes are captured along microtubule walls and then tethered to microtubule-ends through a multi-step end-on conversion process. Here the authors show that Aurora-B regulates end-on conversion in human cells and establish a late role for Astrin-SKAP complex in the end-on conversion process.

Citing Articles

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