The Effect of Intragastric Administration of Botulinum Toxin Type A on Reducing Adiposity in a Rat Model of Obesity Using Micro-CT and Histological Examinations

Overview

Journal Gut Liver

Specialty Gastroenterology

Date 2017 Jul 28

PMID 28750487

Citations 4

Authors

Jin-Seok Park

Hong-Mei Zheng

Jae-Min Kim

Chung Sei Kim

Seok Jeong

Don Haeng Lee

Affiliations

Soon will be listed here.

Abstract

Background/aims: Botulinum toxin type A (BTX), a long-acting inhibitor of muscular contraction in both striated and smooth muscles, is responsible for gastric motility. The aim of this study was to investigate the effects of an endoscopic intragastric BTX injection on weight loss, body fat accumulation, and gastric emptying time.

Methods: The BTX group consisted of 15 obese rats in which 20 U of BTX were injected into the gastric antrum. The saline group consisted of 15 obese rats injected with 20 U of saline, and the control group included 10 obese rats that did not receive a surgical intervention. The gastric emptying time, biochemical parameters, and body fat volume were evaluated using micro-computed tomography (micro-CT) and histologic evaluations.

Results: The postoperative body weight of the BTX group was significantly lower than those of the other groups (p<0.001) at 6 weeks after the operation. The gastric emptying time (156±54 minutes) was significantly delayed in the BTX group. The BTX group showed significantly lower lipid levels than the other groups. A reduction in body fat volume was observed in the BTX group using micro-CT and histological evaluations.

Conclusions: BTX application to the gastric antrum represents a potentially effective treatment for obesity and may help improve the lipid profile by increasing the gastric emptying time.

Citing Articles

Drugs in focus: Botulinum toxin in the therapy of gastrointestinal disorders in children.

Homan M, Thomson M, Bontems P, Saccomani M, Amil Dias J, Faraci S J Pediatr Gastroenterol Nutr. 2024; 79(6):1096-1105.

PMID: 39315663 PMC: 11615126. DOI: 10.1002/jpn3.12376.

Effect of Intragastric Botulinum Type A Injection Combined with a Low-Calorie High-Protein Diet in Adults with Overweight or Obesity.

Hsu P, Wu C, Yang Y, Cheng-Chung Wei J J Clin Med. 2022; 11(12).

PMID: 35743396 PMC: 9225463. DOI: 10.3390/jcm11123325.

The Effect of Botulinum Toxin in Experimental Hypertrophic Pyloric Stenosis.

Sarikaya M, Ciftci I, Unlukal N, Sekmenli T, Gunduz M J Laparoendosc Adv Surg Tech A. 2021; 31(12):1460-1465.

PMID: 34788135 PMC: 8742257. DOI: 10.1089/lap.2021.0308.

How Taking into Account the Pyloric Tonus Contributes to Treatment Success While Administering Gastric "Botulinum Toxin A" for Weight Loss.

Kanlioz M, Ekici U Obes Surg. 2020; 30(9):3365-3369.

PMID: 32405906 DOI: 10.1007/s11695-020-04556-7.

References

Mun E, Blackburn G, Matthews J . Current status of medical and surgical therapy for obesity. Gastroenterology. 2001; 120(3):669-81. DOI: 10.1053/gast.2001.22430. View

Canny J . A computational approach to edge detection. IEEE Trans Pattern Anal Mach Intell. 2011; 8(6):679-98. View

Chevallier J, Zinzindohoue F, Douard R, Blanche J, Berta J, Altman J . Complications after laparoscopic adjustable gastric banding for morbid obesity: experience with 1,000 patients over 7 years. Obes Surg. 2004; 14(3):407-14. DOI: 10.1381/096089204322917954. View

Donnelly K, Smith C, Schwarzenberg S, Jessurun J, Boldt M, Parks E . Sources of fatty acids stored in liver and secreted via lipoproteins in patients with nonalcoholic fatty liver disease. J Clin Invest. 2005; 115(5):1343-51. PMC: 1087172. DOI: 10.1172/JCI23621. View

Xing J, Chen J . Alterations of gastrointestinal motility in obesity. Obes Res. 2004; 12(11):1723-32. DOI: 10.1038/oby.2004.213. View

Kim C, Jang W, Son I, Nam S, Kim Y, Park K . Electrophysiological study for comparing the effect of biological activity between type A botulinum toxins in rat gastrocnemius muscle. Hum Exp Toxicol. 2013; 32(9):914-20. DOI: 10.1177/0960327113479044. View

Cardoso-Junior A, Coelho L, Savassi-Rocha P, Vignolo M, Abrantes M, de Almeida A . Gastric emptying of solids and semi-solids in morbidly obese and non-obese subjects: an assessment using the 13C-octanoic acid and 13C-acetic acid breath tests. Obes Surg. 2007; 17(2):236-41. DOI: 10.1007/s11695-007-9031-4. View

Adams L, Sanderson S, Lindor K, Angulo P . The histological course of nonalcoholic fatty liver disease: a longitudinal study of 103 patients with sequential liver biopsies. J Hepatol. 2005; 42(1):132-8. DOI: 10.1016/j.jhep.2004.09.012. View

Abraham S, Kellow J . Do the digestive tract symptoms in eating disorder patients represent functional gastrointestinal disorders?. BMC Gastroenterol. 2013; 13:38. PMC: 3606125. DOI: 10.1186/1471-230X-13-38. View

10.

Nam H, Park Y, Paik N, Oh B, Chun M, Yang H . Efficacy and safety of NABOTA in post-stroke upper limb spasticity: a phase 3 multicenter, double-blinded, randomized controlled trial. J Neurol Sci. 2015; 357(1-2):192-7. DOI: 10.1016/j.jns.2015.07.028. View

11.

Swinburn B, Sacks G, Hall K, McPherson K, Finegood D, Moodie M . The global obesity pandemic: shaped by global drivers and local environments. Lancet. 2011; 378(9793):804-14. DOI: 10.1016/S0140-6736(11)60813-1. View

12.

Junior A, Savassi-Rocha P, Coelho L, de Mello Sposito M, Albuquerque W, Diniz M . Botulinum A toxin injected into the gastric wall for the treatment of class III obesity: a pilot study. Obes Surg. 2006; 16(3):335-43. DOI: 10.1381/096089206776116408. View

13.

Foschi D, Corsi F, Pisoni L, Vago T, Bevilacqua M, Asti E . Plasma cholecystokinin levels after vertical banded gastroplasty: effects of an acidified meal. Obes Surg. 2004; 14(5):644-7. DOI: 10.1381/096089204323093426. View

14.

James A, Ryan J, Parkman H . Inhibitory effects of botulinum toxin on pyloric and antral smooth muscle. Am J Physiol Gastrointest Liver Physiol. 2003; 285(2):G291-7. DOI: 10.1152/ajpgi.00296.2002. View

15.

Browning J, Baker J, Rogers T, Davis J, Satapati S, Burgess S . Short-term weight loss and hepatic triglyceride reduction: evidence of a metabolic advantage with dietary carbohydrate restriction. Am J Clin Nutr. 2011; 93(5):1048-52. PMC: 3076656. DOI: 10.3945/ajcn.110.007674. View

16.

Cummings D, Weigle D, Frayo R, Breen P, Ma M, Dellinger E . Plasma ghrelin levels after diet-induced weight loss or gastric bypass surgery. N Engl J Med. 2002; 346(21):1623-30. DOI: 10.1056/NEJMoa012908. View

17.

Espinet-Coll E, Nebreda-Duran J, Gomez-Valero J, Munoz-Navas M, Pujol-Gebelli J, Vila-Lolo C . Current endoscopic techniques in the treatment of obesity. Rev Esp Enferm Dig. 2012; 104(2):72-87. DOI: 10.4321/s1130-01082012000200006. View

18.

Coskun H, Duran Y, Dilege E, Mihmanli M, Seymen H, Demirkol M . Effect on gastric emptying and weight reduction of botulinum toxin-A injection into the gastric antral layer: an experimental study in the obese rat model. Obes Surg. 2005; 15(8):1137-43. DOI: 10.1381/0960892055002275. View

19.

Won C, Kim H, Kim B, Kang H, Hong J, Lee S . Comparative trial of a novel botulinum neurotoxin type A versus onabotulinumtoxinA in the treatment of glabellar lines: a multicenter, randomized, double-blind, active-controlled study. Int J Dermatol. 2014; 54(2):227-34. DOI: 10.1111/ijd.12627. View

20.

Cigaina V . Long-term follow-up of gastric stimulation for obesity: the Mestre 8-year experience. Obes Surg. 2004; 14 Suppl 1:S14-22. DOI: 10.1007/BF03342133. View