Potentiating Effects of Karel. on Pentobarbital-induced Sleep

Overview

Journal Avicenna J Phytomed

Publisher Mashhad University of Medical Sciences

Date 2017 Jul 28

PMID 28748168

Citations 1

Authors

Fatemeh Forouzanfar

Azar Hosseini

Mohammad Sadegh Amiri

Hassan Rakhshandeh

Affiliations

Soon will be listed here.

Abstract

Objective: Sleeplessness is the most common sleep disorder. In this study the hypnotic effect of the hydro-alcoholic extract (HAE) of Karel. and its water fraction (WF), ethyl acetate fraction (EAF) and n-butanol fraction (NBF) were studied in mice.

Materials And Methods: The test compounds were administered intraperitoneally to mice 30 min before the administration of sodium pentobarbital (30 mg/kg.). Moreover, the influence of flumazenil on the hypnotic effect of the extracts was evaluated. Besides, 30 min after administration of HAE, motor coordination (rota-rod test) was assessed. Additionally, LD50 for HAE was determined and the possible neurotoxicity of the extract was tested in neural PC12 cells.

Results: The HAE and NBF decreased the latency of sleep (p<0.05), and significantly increased the duration of sleep (p<0.05) induced by pentobarbital. These effects of were reversed by flumazenil. HAE did not affect the animals' performance on the rota-rod test. The LD50 value for HAE was found to be 4.8 g/kg. HAE and its fractions did not show neurotoxic effects in cultured PC12 cell line.

Conclusion: The results showed that significantly potentiated pentobarbital hypnosis without toxic effect. Probably, its effects are related to its non-polar constituents.

Citing Articles

Edible Herbal Medicines as an Alternative to Common Medication for Sleep Disorders: A Review Article.

Hosseini A, Mobasheri L, Rakhshandeh H, Baradaran Rahimi V, Najafi Z, Askari V Curr Neuropharmacol. 2023; 22(7):1205-1232.

PMID: 37345244 PMC: 10964091. DOI: 10.2174/1570159X21666230621143944.

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