Renoprotective Effect of the Shen-Yan-Fang-Shuai Formula by Inhibiting TNF-/NF-B Signaling Pathway in Diabetic Rats

Overview

Journal J Diabetes Res

Publisher Wiley

Specialty Endocrinology

Date 2017 Jul 18

PMID 28713834

Citations 15

Authors

Jie Lv

Zhen Wang

Ying Wang

Weiwei Sun

Jingwei Zhou

Mengdi Wang

Wei Jing Liu

Yaoxian Wang

Affiliations

Soon will be listed here.

Abstract

Diabetic kidney disease (DKD) is the leading cause of end-stage kidney disease, and satisfactory therapeutic strategies have not yet been established. The Shen-Yan-Fang-Shuai Formula (SYFSF) is a traditional Chinese formula composed of Astragali radix, , Baill, and four other herbs. It has been widely used as an effective treatment for DKD patients in China. However, little is known about the molecular mechanisms underlying SYFSF's renoprotection. In this study, we compared the protective effect of SYFSF to irbesartan on the histology and renal cells in type 2 DKD rat model and high-glucose (HG) cultured mesangial cells, respectively. We found that SYFSF could significantly decrease urinary albumin, cholesterol, and triglyceride. And a decrease in serum creatinine was also found in SYFSF-treated group compared with irbesartan-treated rats. In addition, SYFSF inhibited the interstitial expansion and glomerulosclerosis in diabetic rats. Notably, SYFSF markedly downregulated the expression of MCP-1, TGF-1, collagen IV, and fibronectin in diabetic rat models and HG-induced mesangial cell models. The renoprotection was closely associated with a reduced expression of TNF- and phosphorylated NF-Bp65. Our study suggests that SYFSF may ameliorate diabetic kidney injury. The observed renoprotection is probably attributable to an inhibition of inflammatory response and extracellular matrix (ECM) accumulation mediated by TNF-/NF-Bp65 signaling pathway.

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