The Profile of Leucocytes, CD3+, CD4+, and CD8+ T Cells, and Cytokine Concentrations in Peripheral Blood of Children with Acute Asthma Exacerbation

Overview

Journal J Int Med Res

Publisher Sage Publications

Specialty General Medicine

Date 2017 Jul 14

PMID 28703634

Citations 4

Authors

Thuy Nguyen-Thi-Dieu

Huong Le-Thi-Thu

Sy Duong-Quy

Affiliations

Soon will be listed here.

Abstract

Objective To determine the leucocyte profile and cytokine concentrations in the peripheral blood of children with an acute asthma exacerbation (AAE). Methods This descriptive, cross-sectional study enrolled paediatric patients admitted to hospital for AAE. The severity of AAE was assessed using the paediatric asthma score (PAS). Peripheral blood samples were collected for automatic quantification of white blood cell counts, CD3+, CD4+, and CD8+ T cells populations by flow cytometry and cytokine concentrations by flow cytometry-assisted immunoassay. Results A total of 127 children with AAE and 30 healthy control subjects were included in the study. The proportion of paediatric patients with decreased CD3+, CD4+ and CD8+ T cells was significantly higher in those with severe AAE compared with those with mild-to-moderate AAE. The concentrations of interleukin (IL)-2, IL-8, IL-12, and IL-4 in paediatric patients with rhinovirus infection were significantly higher than in those without rhinovirus infection. IL-2, IL-4, IL-6, TNF-α and GM-CSF concentrations during AAE were significantly lower than control. IL-5 and IL-13 concentrations during AAE were significantly higher than control. Conclusions The decrease of CD3+, CD4+, CD8+ T cells and IL-2, IL-4, IL-6, TNF-α, and GM-CSF combined with the increase of IL-5 and IL-13, were associated with AAE in children with asthma.

Citing Articles

Innate immune responses are increased in children with acute asthma exacerbation.

Sheikh S, Eisner M, Walum J, Heyob K, Khan A, Lewis B Pediatr Allergy Immunol. 2024; 35(6):e14173.

PMID: 38873916 PMC: 11182652. DOI: 10.1111/pai.14173.

Clinical Predictors of Wheezing Among Children Infected With .

Kong K, Ding Y, Wu B, Lu M, Gu H Front Pediatr. 2021; 9:693658.

PMID: 34631611 PMC: 8492963. DOI: 10.3389/fped.2021.693658.

Impact of Polyvalent Mechanical Bacterial Lysate on lymphocyte number and activity in asthmatic children: a randomized controlled trial.

Bartkowiak-Emeryk M, Emeryk A, Rolinski J, Wawryk-Gawda E, Markut-Miotla E Allergy Asthma Clin Immunol. 2021; 17(1):10.

PMID: 33472687 PMC: 7816436. DOI: 10.1186/s13223-020-00503-4.

Study of Clinical Characteristics and Cytokine Profiles of Asthmatic Children with Rhinovirus Infection during Acute Asthma Exacerbation at National Hospital of Pediatrics.

Nguyen-Thi-Dieu T, Le-Thi-Thu H, Le-Thi-Minh H, Pham-Nhat A, Duong-Quy S Can Respir J. 2018; 2018:9375967.

PMID: 30210646 PMC: 6126093. DOI: 10.1155/2018/9375967.

References

Woodruff P, Modrek B, Choy D, Jia G, Abbas A, Ellwanger A . T-helper type 2-driven inflammation defines major subphenotypes of asthma. Am J Respir Crit Care Med. 2009; 180(5):388-95. PMC: 2742757. DOI: 10.1164/rccm.200903-0392OC. View

Shi H, Deng J, Xu H, Nong Z, Xiao C, Liu Z . Effect of inhaled interleukin-4 on airway hyperreactivity in asthmatics. Am J Respir Crit Care Med. 1998; 157(6 Pt 1):1818-21. DOI: 10.1164/ajrccm.157.6.9710023. View

Renauld J . New insights into the role of cytokines in asthma. J Clin Pathol. 2001; 54(8):577-89. PMC: 1731485. DOI: 10.1136/jcp.54.8.577. View

Calderon C, Rivera L, Hutchinson P, Dagher H, Villanueva E, Ghildyal R . T-cell cytokine profiles are altered in childhood asthma exacerbation. Respirology. 2009; 14(2):264-9. DOI: 10.1111/j.1440-1843.2008.01464.x. View

Holgate S, Noonan M, Chanez P, Busse W, Dupont L, Pavord I . Efficacy and safety of etanercept in moderate-to-severe asthma: a randomised, controlled trial. Eur Respir J. 2010; 37(6):1352-9. DOI: 10.1183/09031936.00063510. View

Berry M, Hargadon B, Shelley M, Parker D, Shaw D, Green R . Evidence of a role of tumor necrosis factor alpha in refractory asthma. N Engl J Med. 2006; 354(7):697-708. DOI: 10.1056/NEJMoa050580. View

Boudier A, Curjuric I, Basagana X, Hazgui H, Anto J, Bousquet J . Ten-year follow-up of cluster-based asthma phenotypes in adults. A pooled analysis of three cohorts. Am J Respir Crit Care Med. 2013; 188(5):550-60. DOI: 10.1164/rccm.201301-0156OC. View

Berry M, Parker D, Neale N, Woodman L, Morgan A, Monk P . Sputum and bronchial submucosal IL-13 expression in asthma and eosinophilic bronchitis. J Allergy Clin Immunol. 2004; 114(5):1106-9. DOI: 10.1016/j.jaci.2004.08.032. View

Brooks G, Buchta K, Swenson C, Gern J, Busse W . Rhinovirus-induced interferon-gamma and airway responsiveness in asthma. Am J Respir Crit Care Med. 2003; 168(9):1091-4. DOI: 10.1164/rccm.200306-737OC. View

10.

Chiba Y, Nakazawa S, Todoroki M, Shinozaki K, Sakai H, Misawa M . Interleukin-13 augments bronchial smooth muscle contractility with an up-regulation of RhoA protein. Am J Respir Cell Mol Biol. 2008; 40(2):159-67. DOI: 10.1165/rcmb.2008-0162OC. View

11.

Akcakaya N, Sozer V, Cokugras H, Soylemez Y, Yilmaz G . A preliminary study on IL4 levels in extrinsic atopic asthmatic children. Turk J Pediatr. 1994; 36(2):105-10. View

12.

Jamieson K, Warner S, Leigh R, Proud D . Rhinovirus in the Pathogenesis and Clinical Course of Asthma. Chest. 2015; 148(6):1508-1516. DOI: 10.1378/chest.15-1335. View

13.

Schatz M, Hsu J, Zeiger R, Chen W, Dorenbaum A, Chipps B . Phenotypes determined by cluster analysis in severe or difficult-to-treat asthma. J Allergy Clin Immunol. 2013; 133(6):1549-56. DOI: 10.1016/j.jaci.2013.10.006. View

14.

Larche M, Robinson D, Kay A . The role of T lymphocytes in the pathogenesis of asthma. J Allergy Clin Immunol. 2003; 111(3):450-63; quiz 464. DOI: 10.1067/mai.2003.169. View

15.

Ducharme F, Chalut D, Plotnick L, Savdie C, Kudirka D, Zhang X . The Pediatric Respiratory Assessment Measure: a valid clinical score for assessing acute asthma severity from toddlers to teenagers. J Pediatr. 2008; 152(4):476-80, 480.e1. DOI: 10.1016/j.jpeds.2007.08.034. View

16.

Hoekstra M, Hoekstra Y, De Reus D, Rutgers B, Gerritsen J, Kauffman H . Interleukin-4, interferon-gamma and interleukin-5 in peripheral blood of children with moderate atopic asthma. Clin Exp Allergy. 1998; 27(11):1254-60. View

17.

Brown V, Warke T, Shields M, Ennis M . T cell cytokine profiles in childhood asthma. Thorax. 2003; 58(4):311-6. PMC: 1746655. DOI: 10.1136/thorax.58.4.311. View

18.

Murdoch J, Lloyd C . Chronic inflammation and asthma. Mutat Res. 2009; 690(1-2):24-39. PMC: 2923754. DOI: 10.1016/j.mrfmmm.2009.09.005. View

19.

Seder R, Paul W, Davis M, Fazekas de St Groth B . The presence of interleukin 4 during in vitro priming determines the lymphokine-producing potential of CD4+ T cells from T cell receptor transgenic mice. J Exp Med. 1992; 176(4):1091-8. PMC: 2119379. DOI: 10.1084/jem.176.4.1091. View

20.

Brown S, Brown L, Stephenson S, Dodds J, Douglas S, Qu H . Characterization of a high TNF-α phenotype in children with moderate-to-severe asthma. J Allergy Clin Immunol. 2015; 135(6):1651-4. PMC: 4461527. DOI: 10.1016/j.jaci.2014.08.054. View