Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI

Overview

Journal Sci Rep

Specialty Science

Date 2017 Jul 14

PMID 28701710

Citations 16

Authors

Nicolas Santander

Carlos Lizama

Maria Jose Parga

Alonso Quiroz

Druso Perez

Guadalupe Echeverria

Lorena Ulloa

Veronica Palma

Attilio Rigotti

Dolores Busso

Affiliations

Soon will be listed here.

Abstract

SR-BI is the main receptor for high density lipoproteins (HDL) and mediates the bidirectional transport of lipids, such as cholesterol and vitamin E, between these particles and cells. During early development, SR-BI is expressed in extraembryonic tissue, specifically in trophoblast giant cells in the parietal yolk sac. We previously showed that approximately 50% of SR-BI embryos fail to close the anterior neural tube and develop exencephaly, a perinatal lethal condition. Here, we evaluated the role of SR-BI in embryonic vitamin E uptake during murine neural tube closure. Our results showed that SR-BI embryos had a very low vitamin E content in comparison to SR-BI embryos. Whereas SR-BI embryos with closed neural tubes (nSR-BI) had high levels of reactive oxygen species (ROS), intermediate ROS levels between SR-BI and nSR-BI embryos were detected in SR-BI with NTD (NTD SR-BI). Reduced expression of Pax3, Alx1 and Alx3 genes was found in NTD SR-BI embryos. Maternal α-tocopherol dietary supplementation prevented NTD almost completely (from 54% to 2%, p < 0.001) in SR-BI embryos and normalized ROS and gene expression levels. In sum, our results suggest the involvement of SR-BI in the maternal provision of embryonic vitamin E to the mouse embryo during neural tube closure.

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