Mononuclear-macrophages but Not Neutrophils Act As Major Infiltrating Anti-leptospiral Phagocytes During Leptospirosis

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2017 Jul 13

PMID 28700741

Citations 24

Authors

Xu Chen

Shi-Jun Li

David M Ojcius

Ai-Hua Sun

Wei-Lin Hu

Xuai Lin

Jie Yan

Affiliations

Soon will be listed here.

Abstract

Objective: To identify the major infiltrating phagocytes during leptospirosis and examine the killing mechanism used by the host to eliminate Leptospira interrogans.

Methods: Major infiltrating phagocytes in Leptospira-infected C3H/HeJ mice were detected by immunohistochemistry. Chemokines and vascular endothelial cell adhesion molecules (VECAMs) of Leptospira-infected mice and leptospirosis patients were detected by microarray and immunohistochemistry. Leptospira-phagocytosing and -killing abilities of human or mouse macrophages and neutrophils, and the roles of intracellular ROS, NO and [Ca2+]i in Leptospira-killing process were evaluated by confocal microscopy and spectrofluorimetry.

Results: Peripheral blood mononuclear-macrophages rather than neutrophils were the main infiltrating phagocytes in the lungs, liver and kidneys of infected mice. Levels of macrophage- but not neutrophil-specific chemokines and VECAMs were significantly increased in the samples from infected mice and patients. All macrophages tested had a higher ability than neutrophils to phagocytose and kill leptospires. Higher ROS and NO levels and [Ca2+]i in the macrophages were involved in killing leptospires. Human macrophages displayed more phagolysosome formation and a stronger leptospire-killing ability to than mouse macrophages.

Conclusions: Mononuclear-macrophages but not neutrophils represent the main infiltrating and anti-leptospiral phagocytes during leptospirosis. A lower level of phagosome-lysosome fusion may be responsible for the lower Leptospira-killing ability of human macrophages.

Citing Articles

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Correction: Mononuclear-macrophages but not neutrophils act as major infiltrating anti-leptospiral phagocytes during leptospirosis.

Chen X, Li S, Ojcius D, Sun A, Hu W, Lin X PLoS One. 2023; 18(9):e0291717.

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Internalization of Leptospira interrogans via diverse endocytosis mechanisms in human macrophages and vascular endothelial cells.

Zhao X, Guo J, Jia X, Yang Y, Liu L, Nie W PLoS Negl Trop Dis. 2022; 16(9):e0010778.

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Chou L, Chen T, Yang H, Tian Y, Chang M, Hung C Microbiol Spectr. 2022; 10(3):e0259521.

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Campos C, Gomes L, Covarrubias A, Kato E, Souza G, Vasconcellos S Curr Microbiol. 2022; 79(2):57.

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