PiRNA-3878 Targets P450 (CpCYP307B1) to Regulate Pyrethroid Resistance in Culex Pipiens Pallens

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2017 Jul 13

PMID 28698948

Citations 7

Authors

Wenyun Ye

Xianmiao Liu

Juxin Guo

Xueli Sun

Yan Sun

Bo Shen

Dan Zhou

Changliang Zhu

Affiliations

Soon will be listed here.

Abstract

Piwi-interacting RNAs (piRNAs) are a novel class of noncoding single-strand RNAs. They play an important role in the germ cell maintenance, brain development, epigenetic regulation of cancer, and antiviral function. However, little is known about the relationship between the piRNAs and insecticide resistance in mosquitoes. In this study, we reported that piRNA-3878 was related with pyrethroid resistance in Culex pipiens pallens. The expression level of piRNA-3878 was lower in both laboratory and field-collected deltamethrin-resistant (DR) strains. Overexpression of piRNA-3878 increased the susceptibility of the DR strain, while inhibiting the expression of piRNA-3878 in DS strain made the mosquitoes more resistant to deltamethrin. Furthermore, we identified that CpCYP307B1 was the target of piRNA-3878. The mosquito mortality rate was increased after downregulating the expression of CpCYP307B1. These findings revealed that piRNA-3878 could target CpCYP307B1 to regulate pyrethroid resistance.

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