Nucleotide Sequence of the Human N-myc Gene

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Mar 1

PMID 2869488

Citations 67

Authors

L W Stanton

M Schwab

J M Bishop

Affiliations

Soon will be listed here.

Abstract

Human neuroblastomas frequently display amplification and augmented expression of a gene known as N-myc because of its similarity to the protooncogene c-myc. It has therefore been proposed that N-myc is itself a protooncogene, and subsequent tests have shown that N-myc and c-myc have similar biological activities in cell culture. We have now detailed the kinship between N-myc and c-myc by determining the nucleotide sequence of human N-myc and deducing the amino acid sequence of the protein encoded by the gene. The topography of N-myc is strikingly similar to that of c-myc: both genes contain three exons of similar lengths; the coding elements of both genes are located in the second and third exons; and both genes have unusually long 5' untranslated regions in their mRNAs, with features that raise the possibility that expression of the genes may be subject to similar controls of translation. The resemblance between the proteins encoded by N-myc and c-myc sustains previous suspicions that the genes encode related functions.

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References

Brodeur G, Seeger R, Schwab M, Varmus H, Bishop J . Amplification of N-myc in untreated human neuroblastomas correlates with advanced disease stage. Science. 1984; 224(4653):1121-4. DOI: 10.1126/science.6719137. View

Saito H, Hayday A, Wiman K, Hayward W, Tonegawa S . Activation of the c-myc gene by translocation: a model for translational control. Proc Natl Acad Sci U S A. 1983; 80(24):7476-80. PMC: 389974. DOI: 10.1073/pnas.80.24.7476. View

Schwab M, Ellison J, Busch M, Rosenau W, Varmus H, Bishop J . Enhanced expression of the human gene N-myc consequent to amplification of DNA may contribute to malignant progression of neuroblastoma. Proc Natl Acad Sci U S A. 1984; 81(15):4940-4. PMC: 391608. DOI: 10.1073/pnas.81.15.4940. View

Goustin A, Rydnert J, Wahlstrom T, BJERSING L, Stehelin D, Ohlsson R . Spatial and temporal pattern of cellular myc oncogene expression in developing human placenta: implications for embryonic cell proliferation. Cell. 1984; 38(2):585-96. DOI: 10.1016/0092-8674(84)90513-0. View

Greenberg M, Ziff E . Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene. Nature. 1984; 311(5985):433-8. DOI: 10.1038/311433a0. View

Kohl N, Gee C, Alt F . Activated expression of the N-myc gene in human neuroblastomas and related tumors. Science. 1984; 226(4680):1335-7. DOI: 10.1126/science.6505694. View

Thiele C, Reynolds C, Israel M . Decreased expression of N-myc precedes retinoic acid-induced morphological differentiation of human neuroblastoma. Nature. 1985; 313(6001):404-6. DOI: 10.1038/313404a0. View

Darveau A, Pelletier J, Sonenberg N . Differential efficiencies of in vitro translation of mouse c-myc transcripts differing in the 5' untranslated region. Proc Natl Acad Sci U S A. 1985; 82(8):2315-9. PMC: 397548. DOI: 10.1073/pnas.82.8.2315. View

Michitsch R, Melera P . Nucleotide sequence of the 3' exon of the human N-myc gene. Nucleic Acids Res. 1985; 13(7):2545-58. PMC: 341174. DOI: 10.1093/nar/13.7.2545. View

10.

Schwab M, Varmus H, Bishop J . Human N-myc gene contributes to neoplastic transformation of mammalian cells in culture. Nature. 1985; 316(6024):160-2. DOI: 10.1038/316160a0. View

11.

Yancopoulos G, Nisen P, Tesfaye A, Kohl N, Goldfarb M, Alt F . N-myc can cooperate with ras to transform normal cells in culture. Proc Natl Acad Sci U S A. 1985; 82(16):5455-9. PMC: 390588. DOI: 10.1073/pnas.82.16.5455. View

12.

Nau M, Brooks B, Battey J, Sausville E, Gazdar A, Kirsch I . L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer. Nature. 1985; 318(6041):69-73. DOI: 10.1038/318069a0. View

13.

Nau M, Carney D, Battey J, Johnson B, Little C, Gazdar A . Amplification, expression and rearrangement of c-myc and N-myc oncogenes in human lung cancer. Curr Top Microbiol Immunol. 1984; 113:172-7. DOI: 10.1007/978-3-642-69860-6_29. View

14.

Gonda T, Sheiness D, Bishop J . Transcripts from the cellular homologs of retroviral oncogenes: distribution among chicken tissues. Mol Cell Biol. 1982; 2(6):617-24. PMC: 369837. DOI: 10.1128/mcb.2.6.617-624.1982. View

15.

Tinoco Jr I, Borer P, Dengler B, Levin M, Uhlenbeck O, Crothers D . Improved estimation of secondary structure in ribonucleic acids. Nat New Biol. 1973; 246(150):40-1. DOI: 10.1038/newbio246040a0. View

16.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

17.

Messing J, Crea R, Seeburg P . A system for shotgun DNA sequencing. Nucleic Acids Res. 1981; 9(2):309-21. PMC: 326694. DOI: 10.1093/nar/9.2.309. View

18.

Donner P, Greiser-Wilke I, Moelling K . Nuclear localization and DNA binding of the transforming gene product of avian myelocytomatosis virus. Nature. 1982; 296(5854):262-9. DOI: 10.1038/296262a0. View

19.

Mount S . A catalogue of splice junction sequences. Nucleic Acids Res. 1982; 10(2):459-72. PMC: 326150. DOI: 10.1093/nar/10.2.459. View

20.

Collins S, Groudine M . Amplification of endogenous myc-related DNA sequences in a human myeloid leukaemia cell line. Nature. 1982; 298(5875):679-81. DOI: 10.1038/298679a0. View