Infusion of Valproic Acid Into the Renal Medulla Activates Stem Cell Population and Attenuates Salt-Sensitive Hypertension in Dahl S Rats

Overview

Journal Cell Physiol Biochem

Specialties Biochemistry
Cell Biology
Pharmacology

Date 2017 Jul 11

PMID 28693025

Citations 2

Authors

Zhengchao Wang

Qing Zhu

Weili Wang

Fan Yi

Pin-Lan Li

Krishna M Boini

Ningjun Li

Affiliations

Soon will be listed here.

Abstract

Background: Our previous study has detected a stem cell deficiency in the renal medulla in Dahl salt-sensitive (S) rats. This study determined whether infusion of valproic acid (VA), an agent known to stimulate the stem cell function, attenuated salt-sensitive hypertension in Dahl S rats.

Methods: Uninephrectomized Dahl S rats were infused with vehicle or VA (50mg/kg/d) into the renal medulla and fed with a low (LS) or high salt diet (HS). Stem cell marker and number were analyzed by immunohistochemistry, Real-time RT-PCR and Western blot. Sodium excretion and blood pressure were measured.

Results: VA significantly increased the mRNA and protein levels of FGF2, a stem cell niche factor, and CD133, a stem cell marker. The number of CD133+ cells was significantly increased in the renal medulla in VA-treated rats. Meanwhile, high salt-induced increases in the mRNA level of proinflammatory factors interleukin-1β and interleukin-6 were blocked in VA-treated rats. Functionally, sodium excretion in response to the blood pressure increase and acute sodium loading was significantly enhanced, sodium retention attenuated, high salt-induced increase of blood pressure reduced in VA-treated rats.

Conclusion: Activation of stem cell function by VA inhibits the activation of proinflammatory factors and attenuates salt-sensitive hypertension in Dahl S rats.

Citing Articles

Pathophysiology and genetics of salt-sensitive hypertension.

Maaliki D, Itani M, Itani H Front Physiol. 2022; 13:1001434.

PMID: 36176775 PMC: 9513236. DOI: 10.3389/fphys.2022.1001434.

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Tang Z, Huang Y, Zhang Z, Tang Y, Chen J, Sun F Int J Clin Exp Pathol. 2020; 10(12):11384-11392.

PMID: 31966494 PMC: 6966024.

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