Protective Effect of Ganoderma Lucidum Polysaccharide Against Carbon Tetrachloride-induced Hepatic Damage in Precision-cut Carp Liver Slices

Overview

Journal Fish Physiol Biochem

Specialty Biochemistry

Date 2017 Jul 7

PMID 28681206

Citations 2

Authors

Yingjuan Liu

Chunyun Zhang

Jinliang Du

Rui Jia

Liping Cao

Galina Jeney

Hiroki Teraoka

Pao Xu

Guojun Yin

Affiliations

Soon will be listed here.

Abstract

The aim of the present study was to investigate the protective effects of Ganoderma lucidum polysaccharide (GLPS) against carbon tetrachloride (CCl)-induced hepatotoxicity in vitro in common carp. Precision-cut liver slices (PCLSs), which closely resemble the organ from which they are derived, were employed as an in vitro model system. GLPS (0.1, 0.3, and 0.6 mg/ml) was added to PCLS culture system before the exposure to 12 mM CCl. The supernatants and slices were collected to detect molecular and biochemical responses to CCl and PCLS treatments. The levels of CYP1A, CYP3A, and CYP2E1 were measured by ELISA; the mRNA expressions of TNF-α, IL-1β, IL-6, and iNOS were determined by RT-PCR; and the relative protein expressions of c-Rel and p65 were analyzed by western blotting. Results showed that GLPS inhibited the elevations of the marker enzymes (GOT, GPT, LDH) and MDA induced by CCl; it also enhanced the suppressed activity of antioxidant enzymes (SOD, CAT, GSH-Px, T-AOC). The treatment with GLPS resulted in significant downregulation of NF-κB and inflammatory cytokine mRNA levels and significant decreases in the hepatic protein levels of CYP1A, CYP3A, and CYP2E1. These results suggest that GLPS can protect CCl-induced PCLS injury through inhibiting lipid peroxidation, elevating antioxidant enzyme activity, and suppressing immune inflammatory response.

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