Transcriptomic Analysis on Responses of Murine Lungs to Infection

infection in cattle causes serious epidemic diseases and leads to great economic losses in livestock industry; however, little is known about the interaction between host and in the lungs. To explore a fully insight into the host responses in the lungs during infection, a mouse model of pneumonia was established by intraperitoneal infection, and then transcriptomic analysis of infected lungs was performed. localized and grew in murine lungs, and induced inflammation in the lungs, as well as mice death. With transcriptomic analysis, approximately 10 clean reads were acquired. 4236 differently expressed genes (DEGs) were detected during infection, of which 1924 DEGs were up-regulated. By gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichments, 5,303 GO enrichments and 116 KEGG pathways were significantly enriched in the context of infection. Interestingly, genes related to immune responses, such as pattern recognition receptors (PRRs), chemokines and inflammatory cytokines, were significantly up-regulated, suggesting the key roles of these genes in infection. Transcriptomic data showed that IFN-γ/IL-17-related genes were increased, which were validated by qRT-PCR, ELISA, and immunoblotting. Our study characterized the transcriptomic profile of the lungs in mice upon infection, and our findings could provide valuable information with respect to better understanding the responses in mice during infection.