Emodin Protects Against Oxidative Stress and Apoptosis in HK-2 Renal Tubular Epithelial Cells After Hypoxia/reoxygenation

Overview

Journal Exp Ther Med

Specialty Pathology

Date 2017 Jul 5

PMID 28672952

Citations 20

Authors

Hui Chen

Ri-Sheng Huang

Xian-Xian Yu

Qiong Ye

Lu-Lu Pan

Guo-Jian Shao

Jing Pan

Affiliations

Soon will be listed here.

Abstract

The aim of the present study was to determine the effects of emodin, a natural compound with antioxidant properties, on oxidative stress and apoptosis induced by hypoxia/reoxygenation (H/R) in HK-2 human renal tubular cells. In HK-2 cells subjected to H/R, it was observed that pre-treatment with emodin lead to an increase in cellular viability and a reduction in the rate of apoptosis and the B-cell lymphoma 2 (Bcl-2)-associated X protein/Bcl-2 ratio. H/R alone caused a significant increase in the levels of reactive oxygen species and malondialdehyde (P<0.05) and a significant decrease in the activities of superoxide dismutase, catalase and glutathione peroxidase (P<0.05), relative to normoxic cells. In turn, parameters of oxidative stress were improved by emodin pre-treatment. In addition, emodin pre-treatment significantly inhibited the phosphorylation of extracellular signal-regulated protein kinase and c-Jun N-terminal kinase mitogen-activated protein kinases (MAPKs) induced by H/R (P<0.05). These data suggest that emodin may prevent H/R-induced apoptosis in human renal tubular cells through the regulation of cellular oxidative stress, MAPK activation and restoration of the Bax/Bcl-2 ratio.

Citing Articles

Emodin improves the cardiac function in the rats with chronic heart failure through regulation of the miR-26b-5p/PTEN pathway.

Gu Z, Zhang S, Zhao S, Cui Y, Sun L Arch Med Sci. 2024; 20(2):655-663.

PMID: 38757011 PMC: 11094829. DOI: 10.5114/aoms.2020.96345.

Emodin prevents renal ischemia-reperfusion injury via suppression of p53-mediated cell apoptosis based on network pharmacology.

Lu H, Xie D, Qu B, Li M, He Y, Liu W Heliyon. 2023; 9(5):e15682.

PMID: 37215853 PMC: 10195913. DOI: 10.1016/j.heliyon.2023.e15682.

The In Vitro Anti-Parasitic Activities of Emodin toward .

Adeyemi O, Ishii K, Kato K Pharmaceuticals (Basel). 2023; 16(3).

PMID: 36986545 PMC: 10053859. DOI: 10.3390/ph16030447.

Cerium oxide-based nanozyme suppresses kidney calcium oxalate crystal depositions via reversing hyperoxaluria-induced oxidative stress damage.

Deng J, Yu B, Chang Z, Wu S, Li G, Chen W J Nanobiotechnology. 2022; 20(1):516.

PMID: 36482378 PMC: 9733203. DOI: 10.1186/s12951-022-01726-w.

The progress and prospect of natural components in rhubarb () in the treatment of renal fibrosis.

Wang Y, Yu F, Li A, He Z, Qu C, He C Front Pharmacol. 2022; 13:919967.

PMID: 36105187 PMC: 9465315. DOI: 10.3389/fphar.2022.919967.

References

Bae E, Cho S, Joo S, Ma S, Kim S, Lee J . 4-Hydroxy-2-hexenal-induced apoptosis in human renal proximal tubular epithelial cells. Nephrol Dial Transplant. 2011; 26(12):3866-73. DOI: 10.1093/ndt/gfr386. View

Lee D, Park S, Bae S, Jeong D, Park M, Kang C . Hydrogen peroxide-activatable antioxidant prodrug as a targeted therapeutic agent for ischemia-reperfusion injury. Sci Rep. 2015; 5:16592. PMC: 4643254. DOI: 10.1038/srep16592. View

Cai Q, Ma T, Li C, Tian Y, Li H . Catalpol Protects Pre-Myelinating Oligodendrocytes against Ischemia-induced Oxidative Injury through ERK1/2 Signaling Pathway. Int J Biol Sci. 2016; 12(12):1415-1426. PMC: 5166484. DOI: 10.7150/ijbs.16823. View

Du Y, Ko K . Effects of emodin treatment on mitochondrial ATP generation capacity and antioxidant components as well as susceptibility to ischemia-reperfusion injury in rat hearts: single versus multiple doses and gender difference. Life Sci. 2005; 77(22):2770-82. DOI: 10.1016/j.lfs.2005.03.027. View

Guan X, Qian Y, Shen Y, Zhang L, Du Y, Dai H . Autophagy protects renal tubular cells against ischemia / reperfusion injury in a time-dependent manner. Cell Physiol Biochem. 2015; 36(1):285-98. DOI: 10.1159/000374071. View

Aichner D, Ganzera M . Analysis of anthraquinones in rhubarb (Rheum palmatum and Rheum officinale) by supercritical fluid chromatography. Talanta. 2015; 144:1239-44. DOI: 10.1016/j.talanta.2015.08.011. View

Kvansakul M, Hinds M . The Bcl-2 family: structures, interactions and targets for drug discovery. Apoptosis. 2014; 20(2):136-50. DOI: 10.1007/s10495-014-1051-7. View

Park S, Jin M, Ko M, Park G, Choi Y . Anti-neuroinflammatory Effect of Emodin in LPS-Stimulated Microglia: Involvement of AMPK/Nrf2 Activation. Neurochem Res. 2016; 41(11):2981-2992. DOI: 10.1007/s11064-016-2018-6. View

Chen R, Zhang J, Hu Y, Wang S, Chen M, Wang Y . Potential antineoplastic effects of Aloe-emodin: a comprehensive review. Am J Chin Med. 2014; 42(2):275-88. DOI: 10.1142/S0192415X14500189. View

10.

Nemmar A, Al-Salam S, Yuvaraju P, Beegam S, Ali B . Emodin mitigates diesel exhaust particles-induced increase in airway resistance, inflammation and oxidative stress in mice. Respir Physiol Neurobiol. 2015; 215:51-7. DOI: 10.1016/j.resp.2015.05.006. View

11.

Suzuki C, Isaka Y, Shimizu S, Tsujimoto Y, Takabatake Y, Ito T . Bcl-2 protects tubular epithelial cells from ischemia reperfusion injury by inhibiting apoptosis. Cell Transplant. 2008; 17(1-2):223-9. DOI: 10.3727/000000008783907053. View

12.

Son Y, Cheong Y, Kim N, Chung H, Kang D, Pae H . Mitogen-Activated Protein Kinases and Reactive Oxygen Species: How Can ROS Activate MAPK Pathways?. J Signal Transduct. 2011; 2011:792639. PMC: 3100083. DOI: 10.1155/2011/792639. View

13.

Wu Y, Tu X, Lin G, Xia H, Huang H, Wan J . Emodin-mediated protection from acute myocardial infarction via inhibition of inflammation and apoptosis in local ischemic myocardium. Life Sci. 2007; 81(17-18):1332-8. DOI: 10.1016/j.lfs.2007.08.040. View

14.

Kunduzova O, Bianchi P, Pizzinat N, Escourrou G, Seguelas M, Parini A . Regulation of JNK/ERK activation, cell apoptosis, and tissue regeneration by monoamine oxidases after renal ischemia-reperfusion. FASEB J. 2002; 16(9):1129-31. DOI: 10.1096/fj.01-1008fje. View

15.

Zhang Y, Liao H, Zhong S, Gao F, Chen Y, Huang Z . Effect of N-n-butyl haloperidol iodide on ROS/JNK/Egr-1 signaling in H9c2 cells after hypoxia/reoxygenation. Sci Rep. 2015; 5:11809. PMC: 4488875. DOI: 10.1038/srep11809. View

16.

Zhou T, Chuang C, Zuo L . Molecular Characterization of Reactive Oxygen Species in Myocardial Ischemia-Reperfusion Injury. Biomed Res Int. 2015; 2015:864946. PMC: 4609796. DOI: 10.1155/2015/864946. View

17.

Xue W, Shi X, Liang S, Zhou L, Liu K, Zhao J . Emodin Attenuates Cigarette Smoke Induced Lung Injury in a Mouse Model via Suppression of Reactive Oxygen Species Production. J Biochem Mol Toxicol. 2015; 29(11):526-32. DOI: 10.1002/jbt.21723. View

18.

Duan J, Guan Y, Mu F, Guo C, Zhang E, Yin Y . Protective effect of butin against ischemia/reperfusion-induced myocardial injury in diabetic mice: involvement of the AMPK/GSK-3β/Nrf2 signaling pathway. Sci Rep. 2017; 7:41491. PMC: 5269748. DOI: 10.1038/srep41491. View

19.

Zu G, Guo J, Che N, Zhou T, Zhang X, Wang G . Protective effects of ginsenoside Rg1 on intestinal ischemia/reperfusion injury-induced oxidative stress and apoptosis via activation of the Wnt/β-catenin pathway. Sci Rep. 2016; 6:38480. PMC: 5133596. DOI: 10.1038/srep38480. View

20.

Yin J, Wan B, Liu D, Wan S, Fu H, Wan Y . Emodin alleviates lung injury in rats with sepsis. J Surg Res. 2016; 202(2):308-14. DOI: 10.1016/j.jss.2015.12.049. View