Fluorescent, Plasmonic, and Radiotherapeutic Properties of the Lu-Dendrimer-AuNP-Folate-Bombesin Nanoprobe Located Inside Cancer Cells

Overview

Journal Mol Imaging

Publisher Sage Publications

Specialty Radiology

Date 2017 Jun 28

PMID 28654384

Citations 18

Authors

Hector Mendoza-Nava

Guillermina Ferro-Flores

Flor de Maria Ramirez

Blanca Ocampo-Garcia

Clara Santos-Cuevas

Erika Azorin-Vega

Nallely Jimenez-Mancilla

Myrna Luna-Gutierrez

Keila Isaac-Olive

Affiliations

Soon will be listed here.

Abstract

The integration of fluorescence and plasmonic properties into one molecule is of importance in developing multifunctional imaging and therapy nanoprobes. The aim of this research was to evaluate the fluorescent properties and the plasmonic-photothermal, therapeutic, and radiotherapeutic potential of Lu-dendrimer conjugated to folate and bombesin with gold nanoparticles in the dendritic cavity (Lu-DenAuNP-folate-bombesin) when it is internalized in T47D breast cancer cells. The intense near-Infrared (NIR) fluorescence emitted at 825 nm from the conjugate inside cells corroborated the usefulness of DenAuNP-folate-bombesin for optical imaging. After laser irradiation, the presence of the nanosystem in cells caused a significant increase in the temperature of the medium (46.8°C, compared to 39.1°C without DenAuNP-folate-bombesin, P < 0.05), resulting in a significant decrease in cell viability (down to 16.51% ± 1.52%) due to the Lu-DenAuNP-folate-bombesin plasmonic properties. After treatment with Lu-DenAuNP-folate-bombesin, the T47D cell viability decreased 90% because of the radiation-absorbed dose (63.16 ± 4.20 Gy) delivered inside the cells. The Lu-DenAuNP-folate-bombesin nanoprobe internalized in cancer cells exhibited properties suitable for optical imaging, plasmonic-photothermal therapy, and targeted radiotherapy.

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References

Jimenez-Mancilla N, Ferro-Flores G, Santos-Cuevas C, Ocampo-Garcia B, Luna-Gutierrez M, Azorin-Vega E . Multifunctional targeted therapy system based on (99m) Tc/(177) Lu-labeled gold nanoparticles-Tat(49-57)-Lys(3) -bombesin internalized in nuclei of prostate cancer cells. J Labelled Comp Radiopharm. 2014; 56(13):663-71. DOI: 10.1002/jlcr.3087. View

Sanchez-Hernandez L, Ferro-Flores G, Jimenez-Mancilla N, Luna-Gutierrez M, Santos-Cuevas C, Ocampo-Garcia B . Comparative Effect Between Laser and Radiofrequency Heating of RGD-Gold Nanospheres on MCF7 Cell Viability. J Nanosci Nanotechnol. 2015; 15(12):9840-8. DOI: 10.1166/jnn.2015.10328. View

Mendoza-Nava H, Ferro-Flores G, Ocampo-Garcia B, Serment-Guerrero J, Santos-Cuevas C, Jimenez-Mancilla N . Laser heating of gold nanospheres functionalized with octreotide: in vitro effect on HeLa cell viability. Photomed Laser Surg. 2012; 31(1):17-22. DOI: 10.1089/pho.2012.3320. View

Goswami N, Yao Q, Luo Z, Li J, Chen T, Xie J . Luminescent Metal Nanoclusters with Aggregation-Induced Emission. J Phys Chem Lett. 2016; 7(6):962-75. DOI: 10.1021/acs.jpclett.5b02765. View

Surujpaul P, Gutierrez-Wing C, Ocampo-Garcia B, Ramirez F, Arteaga de Murphy C, Pedraza-Lopez M . Gold nanoparticles conjugated to [Tyr3]octreotide peptide. Biophys Chem. 2008; 138(3):83-90. DOI: 10.1016/j.bpc.2008.09.005. View

Fay B, Melamed J, Day E . Nanoshell-mediated photothermal therapy can enhance chemotherapy in inflammatory breast cancer cells. Int J Nanomedicine. 2015; 10:6931-41. PMC: 4644159. DOI: 10.2147/IJN.S93031. View

Zheng J, Zhou C, Yu M, Liu J . Different sized luminescent gold nanoparticles. Nanoscale. 2012; 4(14):4073-83. PMC: 3404491. DOI: 10.1039/c2nr31192e. View

Ferro-Flores G, Ocampo-Garcia B, Santos-Cuevas C, Morales-Avila E, Azorin-Vega E . Multifunctional radiolabeled nanoparticles for targeted therapy. Curr Med Chem. 2013; 21(1):124-38. DOI: 10.2174/09298673113209990218. View

Letfullin R, Iversen C, George T . Modeling nanophotothermal therapy: kinetics of thermal ablation of healthy and cancerous cell organelles and gold nanoparticles. Nanomedicine. 2010; 7(2):137-45. DOI: 10.1016/j.nano.2010.06.011. View

10.

Orocio-Rodriguez E, Ferro-Flores G, Santos-Cuevas C, Ramirez F, Ocampo-Garcia B, Azorin-Vega E . Two Novel Nanosized Radiolabeled Analogues of Somatostatin for Neuroendocrine Tumor Imaging. J Nanosci Nanotechnol. 2015; 15(6):4159-69. DOI: 10.1166/jnn.2015.9620. View

11.

Sancho V, Di Florio A, Moody T, Jensen R . Bombesin receptor-mediated imaging and cytotoxicity: review and current status. Curr Drug Deliv. 2010; 8(1):79-134. PMC: 3058932. DOI: 10.2174/156720111793663624. View

12.

Banerjee S, Pillai M, Knapp F . Lutetium-177 therapeutic radiopharmaceuticals: linking chemistry, radiochemistry, and practical applications. Chem Rev. 2015; 115(8):2934-74. DOI: 10.1021/cr500171e. View

13.

Astruc D, Boisselier E, Ornelas C . Dendrimers designed for functions: from physical, photophysical, and supramolecular properties to applications in sensing, catalysis, molecular electronics, photonics, and nanomedicine. Chem Rev. 2010; 110(4):1857-959. DOI: 10.1021/cr900327d. View

14.

Necela B, Crozier J, Andorfer C, Lewis-Tuffin L, Kachergus J, Geiger X . Folate receptor-α (FOLR1) expression and function in triple negative tumors. PLoS One. 2015; 10(3):e0122209. PMC: 4376802. DOI: 10.1371/journal.pone.0122209. View

15.

Aranda-Lara L, Ferro-Flores G, Azorin-Vega E, Ramirez F, Jimenez-Mancilla N, Ocampo-Garcia B . Synthesis and evaluation of Lys¹(α,γ-Folate)Lys³(¹⁷⁷Lu-DOTA)-Bombesin(1-14) as a potential theranostic radiopharmaceutical for breast cancer. Appl Radiat Isot. 2015; 107:214-219. DOI: 10.1016/j.apradiso.2015.10.030. View

16.

Dalm S, Martens J, Sieuwerts A, van Deurzen C, Koelewijn S, de Blois E . In vitro and in vivo application of radiolabeled gastrin-releasing peptide receptor ligands in breast cancer. J Nucl Med. 2015; 56(5):752-7. DOI: 10.2967/jnumed.114.153023. View

17.

OShannessy D, Somers E, Maltzman J, Smale R, Fu Y . Folate receptor alpha (FRA) expression in breast cancer: identification of a new molecular subtype and association with triple negative disease. Springerplus. 2013; 1:22. PMC: 3725886. DOI: 10.1186/2193-1801-1-22. View

18.

Zhou C, Yang S, Liu J, Yu M, Zheng J . Luminescent gold nanoparticles: a new class of nanoprobes for biomedical imaging. Exp Biol Med (Maywood). 2013; 238(11):1199-209. DOI: 10.1177/1535370213505825. View

19.

Zhu J, Zheng L, Wen S, Tang Y, Shen M, Zhang G . Targeted cancer theranostics using alpha-tocopheryl succinate-conjugated multifunctional dendrimer-entrapped gold nanoparticles. Biomaterials. 2014; 35(26):7635-46. DOI: 10.1016/j.biomaterials.2014.05.046. View

20.

Oh E, Hong M, Lee D, Nam S, Yoon H, Kim H . Inhibition assay of biomolecules based on fluorescence resonance energy transfer (FRET) between quantum dots and gold nanoparticles. J Am Chem Soc. 2005; 127(10):3270-1. DOI: 10.1021/ja0433323. View