Identification of a Novel Fungus, Trichoderma Asperellum GDFS1009, and Comprehensive Evaluation of Its Biocontrol Efficacy

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2017 Jun 24

PMID 28644879

Citations 32

Authors

Qiong Wu

Ruiyan Sun

Mi Ni

Jia Yu

Yaqian Li

Chuanjin Yu

Kai Dou

Jianhong Ren

Jie Chen

Affiliations

Soon will be listed here.

Abstract

Due to its efficient broad-spectrum antimicrobial activity, Trichoderma has been established as an internationally recognized biocontrol fungus. In this study, we found and identified a novel strain of Trichoderma asperellum, named GDFS1009. The mycelium of T. asperellum GDFS1009 exhibits a high growth rate, high sporulation capacity, and strong inhibitory effects against pathogens that cause cucumber fusarium wilt and corn stalk rot. T. asperellum GDFS1009 secretes chitinase, glucanase, and protease, which can degrade the cell walls of fungi and contribute to mycoparasitism. The secreted xylanases are good candidates for inducing plant resistance and enhancing plant immunity against pathogens. RNA sequencing (RNA-seq) and gas chromatography-mass spectrometry (GC-MS) showed that T. asperellum GDFS1009 produces primary metabolites that are precursors of antimicrobial compounds; it also produces a variety of antimicrobial secondary metabolites, including polyketides and alkanes. In addition, this study speculated the presence of six antimicrobial peptides via ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF-MS/MS). Future studies should focus on these antimicrobial metabolites for facilitating widespread application in the field of agricultural bio-control.

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