The Investigation of Electrochemistry Behaviors of Tyrosinase Based on Directly-Electrodeposited Grapheneon Choline-Gold Nanoparticles

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2017 Jun 24

PMID 28644401

Citations 2

Authors

Yaping He

Xiaohui Yang

Quan Han

Jianbin Zheng

Affiliations

Soon will be listed here.

Abstract

A novel catechol (CA) biosensor was developed by embedding tyrosinase (Tyr) onto in situ electrochemical reduction graphene (EGR) on choline-functionalized gold nanoparticle (AuNPs-Ch) film. The results of UV-Vis spectra indicated that Tyr retained its original structure in the film, and an electrochemical investigation of the biosensor showed a pair of well-defined, quasi-reversible redox peaks with = -0.0744 V and = -0.114 V (vs. SCE) in 0.1 M, pH 7.0 sodium phosphate-buffered saline at a scan rate of 100 mV/s. The transfer rate constant is 0.66 s. The Tyr-EGR/AuNPs-Ch showed a good electrochemical catalytic response for the reduction of CA, with the linear range from 0.2 to 270 μM and a detection limit of 0.1 μM (S/N = 3). The apparent Michaelis-Menten constant was estimated to be 109 μM.

Citing Articles

Development of a Novel Biosensor Based on Tyrosinase/Platinum Nanoparticles/Chitosan/Graphene Nanostructured Layer with Applicability in Bioanalysis.

Apetrei I, Apetrei C Materials (Basel). 2019; 12(7).

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Electrochemical Screening and Evaluation of Lamiaceae Plant Species from South Africa with Potential Tyrosinase Activity.

Etsassala N, Waryo T, Popoola O, Adeloye A, Iwuoha E, Hussein A Sensors (Basel). 2019; 19(5).

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References

Tembe S, Inamdar S, Haram S, Karve M, DSouza S . Electrochemical biosensor for catechol using agarose-guar gum entrapped tyrosinase. J Biotechnol. 2006; 128(1):80-5. DOI: 10.1016/j.jbiotec.2006.09.020. View

Yang L, Xiong H, Zhang X, Wang S . A novel tyrosinase biosensor based on chitosan-carbon-coated nickel nanocomposite film. Bioelectrochemistry. 2011; 84:44-8. DOI: 10.1016/j.bioelechem.2011.11.001. View

Yin H, Zhou Y, Xu J, Ai S, Cui L, Zhu L . Amperometric biosensor based on tyrosinase immobilized onto multiwalled carbon nanotubes-cobalt phthalocyanine-silk fibroin film and its application to determine bisphenol A. Anal Chim Acta. 2010; 659(1-2):144-50. DOI: 10.1016/j.aca.2009.11.051. View

Lu L, Zhang L, Zhang X, Huan S, Shen G, Yu R . A novel tyrosinase biosensor based on hydroxyapatite-chitosan nanocomposite for the detection of phenolic compounds. Anal Chim Acta. 2010; 665(2):146-51. DOI: 10.1016/j.aca.2010.03.033. View

Chen D, Tang L, Li J . Graphene-based materials in electrochemistry. Chem Soc Rev. 2010; 39(8):3157-80. DOI: 10.1039/b923596e. View

Wang P, Li Y, Huang X, Wang L . Fabrication of layer-by-layer modified multilayer films containing choline and gold nanoparticles and its sensing application for electrochemical determination of dopamine and uric acid. Talanta. 2008; 73(3):431-7. DOI: 10.1016/j.talanta.2007.04.022. View

Guix M, Perez-Lopez B, Sahin M, Roldan M, Ambrosi A, Merkoci A . Structural characterization by confocal laser scanning microscopy and electrochemical study of multi-walled carbon nanotube tyrosinase matrix for phenol detection. Analyst. 2010; 135(8):1918-25. DOI: 10.1039/c000929f. View

Jang E, Son K, Kim B, Koh W . Phenol biosensor based on hydrogel microarrays entrapping tyrosinase and quantum dots. Analyst. 2010; 135(11):2871-8. DOI: 10.1039/c0an00353k. View

Adams K, Jena B, Percival S, Zhang B . Highly sensitive detection of exocytotic dopamine release using a gold-nanoparticle-network microelectrode. Anal Chem. 2010; 83(3):920-7. DOI: 10.1021/ac102599s. View

10.

Burestedt E, Narvaez A, Ruzgas T, Gorton L, Emneus J, Dominguez E . Rate-limiting steps of tyrosinase-modified electrodes for the detection of catechol. Anal Chem. 2011; 68(9):1605-11. DOI: 10.1021/ac950742o. View

11.

Shu F, Wilson G . Rotating ring-disk enzyme electrode for surface catalysis studies. Anal Chem. 1976; 48(12):1679-86. DOI: 10.1021/ac50006a014. View

12.

Unnikrishnan B, Palanisamy S, Chen S . A simple electrochemical approach to fabricate a glucose biosensor based on graphene-glucose oxidase biocomposite. Biosens Bioelectron. 2012; 39(1):70-5. DOI: 10.1016/j.bios.2012.06.045. View

13.

Zhou M, Zhai Y, Dong S . Electrochemical sensing and biosensing platform based on chemically reduced graphene oxide. Anal Chem. 2009; 81(14):5603-13. DOI: 10.1021/ac900136z. View

14.

Pandey P, Singh S, Arya S, Gupta V, Datta M, Singh S . Application of thiolated gold nanoparticles for the enhancement of glucose oxidase activity. Langmuir. 2007; 23(6):3333-7. DOI: 10.1021/la062901c. View

15.

Guo H, Wang X, Qian Q, Wang F, Xia X . A green approach to the synthesis of graphene nanosheets. ACS Nano. 2009; 3(9):2653-9. DOI: 10.1021/nn900227d. View

16.

Kannan P, John S . Determination of nanomolar uric and ascorbic acids using enlarged gold nanoparticles modified electrode. Anal Biochem. 2008; 386(1):65-72. DOI: 10.1016/j.ab.2008.11.043. View

17.

Ye B, Zhou X . Direct electrochemical redox of tyrosinase at silver electrodes. Talanta. 1997; 44(5):831-6. DOI: 10.1016/s0039-9140(96)02121-2. View

18.

Luo J, Jiang S, Zhang H, Jiang J, Liu X . A novel non-enzymatic glucose sensor based on Cu nanoparticle modified graphene sheets electrode. Anal Chim Acta. 2011; 709:47-53. DOI: 10.1016/j.aca.2011.10.025. View

19.

Du W, Zhao F, Zeng B . Novel multiwalled carbon nanotubes-polyaniline composite film coated platinum wire for headspace solid-phase microextraction and gas chromatographic determination of phenolic compounds. J Chromatogr A. 2009; 1216(18):3751-7. DOI: 10.1016/j.chroma.2009.03.013. View

20.

Du D, Zou Z, Shin Y, Wang J, Wu H, Engelhard M . Sensitive immunosensor for cancer biomarker based on dual signal amplification strategy of graphene sheets and multienzyme functionalized carbon nanospheres. Anal Chem. 2010; 82(7):2989-95. PMC: 2909472. DOI: 10.1021/ac100036p. View