Adenosine Triphosphate-Encapsulated Liposomes with Plasmonic Nanoparticles for Surface Enhanced Raman Scattering-Based Immunoassays

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2017 Jun 24

PMID 28644380

Citations 2

Authors

Xuan-Hung Pham

Eunil Hahm

Tae Han Kim

Hyung-Mo Kim

Sang Hun Lee

Yoon-Sik Lee

Dae Hong Jeong

Bong-Hyun Jun

Affiliations

Soon will be listed here.

Abstract

In this study, we prepared adenosine triphosphate (ATP) encapsulated liposomes, and assessed their applicability for the surface enhanced Raman scattering (SERS)-based assays with gold-silver alloy (Au@Ag)-assembled silica nanoparticles (NPs; SiO₂@Au@Ag). The liposomes were prepared by the thin film hydration method from a mixture of l-α-phosphatidylcholine, cholesterol, and PE-PEG2000 in chloroform; evaporating the solvent, followed by hydration of the resulting thin film with ATP in phosphate-buffered saline (PBS). Upon lysis of the liposome, the SERS intensity of the SiO₂@Au@Ag NPs increased with the logarithm of number of ATP-encapsulated liposomes after lysis in the range of 8 × 10⁶ to 8 × 10. The detection limit of liposome was calculated to be 1.3 × 10 mol. The successful application of ATP-encapsulated liposomes to SiO₂@Au@Ag NPs based SERS analysis has opened a new avenue for Raman label chemical (RCL)-encapsulated liposome-enhanced SERS-based immunoassays.

Citing Articles

Recent Studies on Metal-Embedded Silica Nanoparticles for Biological Applications.

Cho H, Noh M, Kim Y, Namgung J, Yoo K, Shin M Nanomaterials (Basel). 2024; 14(3).

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Microfluidic SERS devices: brightening the future of bioanalysis.

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