Levels of Neutrophil Gelatinase-assosciated Lipocalin in Patients with Head and Neck Squamous Cell Carcinoma in Indian Population from Haryana State

Aim: To study the levels of neutrophil gelatinase associated lipocalin (NGAL) in head and neck squamous cell carcinoma (HNSCC).

Methods: This was a non randomized case control study conducted at Department of Biochemistry, in collaboration with Regional Cancer Center over a period of one year. The study population included 50 adult newly diagnosed HNSCC patients reporting in outpatient department at Regional Cancer Center and compared with 50 healthy controls. NGAL was estimated by ELISA technique. Student test and test were applied for comparison of means of study groups. Correlations between groups were analyzed using Pearson correlation coefficient () formula.

Results: Patients with HNSCC exhibited significantly increased levels of NGAL ( < 0.05) as compared to healthy controls (978.88 ± 261.39 ng/mL 34.83 ± 7.59 ng/mL). Out of 50, 26 patients (52%) were in stage IV, 21 (42%) in stage III, 1 (2%) patient in stage II and 2 (4%) patients were in stage I. Metastasis was absent in 98% patients and mean NGAL levels were highest in these patients but value was not significant. Mean NGAL levels were highest in stage IV [1041.54 ± 222.15 ng/mL (stage IV) 1040 ± 0.00 ng/mL (stage I); 900 ± 0.00 ng/mL (stage II) and 1031.90 ± 202.55 ng/mL (stage III)] and test was highly significant ( < 0.001). Thirty-six patients (72%) were having moderately differentiated HNSCC and mean NGAL levels were maximum in patients with well differentiated HNSCC (1164 ± 315.64 ng/mL 1013.33 ± 161.19 ng/mL in moderately differentiated and 890 ± 11.55 ng/mL in poorly differentiated) and the results were also highly significant ( < 0.001, test).

Conclusion: The present work demonstrates a potential role of NGAL as cancer biomarker and its use in monitoring the HNSCC progression.