Gene Expression Profiling Reveals Potential Players of Left-Right Asymmetry in Female Chicken Gonads

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2017 Jun 21

PMID 28632173

Citations 9

Authors

Zhiyi Wan

Yanan Lu

Lei Rui

Xiaoxue Yu

Fang Yang

Chengfang Tu

Zandong Li

Affiliations

Soon will be listed here.

Abstract

Most female birds develop only a left ovary, whereas males develop bilateral testes. The mechanism underlying this process is still not completely understood. Here, we provide a comprehensive transcriptional analysis of female chicken gonads and identify novel candidate side-biased genes. RNA-Seq analysis was carried out on total RNA harvested from the left and right gonads on embryonic day 6 (E6), E12, and post-hatching day 1 (D1). By comparing the gene expression profiles between the left and right gonads, 347 differentially expressed genes (DEGs) were obtained on E6, 3730 were obtained on E12, and 2787 were obtained on D1. Side-specific genes were primarily derived from the autosome rather than the sex chromosome. Gene ontology and pathway analysis showed that the DEGs were most enriched in the Piwi-interactiing RNA (piRNA) metabolic process, germ plasm, chromatoid body, P granule, neuroactive ligand-receptor interaction, microbial metabolism in diverse environments, and methane metabolism. A total of 111 DEGs, five gene ontology (GO) terms, and three pathways were significantly different between the left and right gonads among all the development stages. We also present the gene number and the percentage within eight development-dependent expression patterns of DEGs in the left and right gonads of female chicken.

Citing Articles

Gene Expression Profiling Reveals Potential Players of Sex Determination and Asymmetrical Development in Chicken Embryo Gonads.

Luo H, Zhou H, Jiang S, He C, Xu K, Ding J Int J Mol Sci. 2023; 24(19).

PMID: 37834055 PMC: 10572726. DOI: 10.3390/ijms241914597.

Exploring right ovary degeneration in duck and goose embryos by histology and transcriptome dynamics analysis.

Ran M, Ouyang Q, Li X, Hu S, Hu B, Hu J BMC Genomics. 2023; 24(1):389.

PMID: 37430218 PMC: 10332064. DOI: 10.1186/s12864-023-09493-0.

A PITX2-HTR1B pathway regulates the asymmetric development of female gonads in chickens.

Peng Z, Man Q, Meng L, Wang S, Cai H, Zhang C PNAS Nexus. 2023; 2(6):pgad202.

PMID: 37388922 PMC: 10304771. DOI: 10.1093/pnasnexus/pgad202.

Time-Course Transcriptional and Chromatin Accessibility Profiling Reveals Genes Associated With Asymmetrical Gonadal Development in Chicken Embryos.

Li J, Sun C, Zheng J, Li J, Yi G, Yang N Front Cell Dev Biol. 2022; 10:832132.

PMID: 35345851 PMC: 8957256. DOI: 10.3389/fcell.2022.832132.

H3K27ac chromatin acetylation and gene expression analysis reveal sex- and situs-related differences in developing chicken gonads.

Jiang Y, Peng Z, Man Q, Wang S, Huang X, Meng L Biol Sex Differ. 2022; 13(1):6.

PMID: 35135592 PMC: 8822763. DOI: 10.1186/s13293-022-00415-5.

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