Appearance of Cell-adhesion Factor in Osteoblast Proliferation and Differentiation of Apatite Coating Titanium by Blast Coating Method

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2017 Jun 17

PMID 28620829

Citations 2

Authors

Hirotsugu Umeda

Takamitsu Mano

Koji Harada

Ferdous Tarannum

Yoshiya Ueyama

Affiliations

Soon will be listed here.

Abstract

We have already reported that the apatite coating of titanium by the blast coating (BC) method could show a higher rate of bone contact from the early stages in vivo, when compared to the pure titanium (Ti) and the apatite coating of titanium by the flame spraying (FS) method. However, the detailed mechanism by which BC resulted in satisfactory bone contact is still unknown. In the present study, we investigated the importance of various factors including cell adhesion factor in osteoblast proliferation and differentiation that could affect the osteoconductivity of the BC disks. Cell proliferation assay revealed that Saos-2 could grow fastest on BC disks, and that a spectrophotometric method using a LabAssay ALP kit showed that ALP activity was increased in cells on BC disks compared to Ti disks and FS disks. In addition, higher expression of E-cadherin and Fibronectin was observed in cells on BC disks than Ti disks and FS disks by relative qPCR as well as Western blotting. These results suggested that the expression of cell-adhesion factors, proliferation and differentiation of osteoblast might be enhanced on BC disks, which might result higher osteoconductivity.

Citing Articles

Surface modifications and coatings to improve osseointegration and antimicrobial activity on titanium surfaces: A statistical review over the last decade.

Raju K, Biswas A J Orthop. 2025; 67:68-87.

PMID: 39902142 PMC: 11787716. DOI: 10.1016/j.jor.2025.01.002.

Implant-bone-interface: Reviewing the impact of titanium surface modifications on osteogenic processes in vitro and in vivo.

Stich T, Alagboso F, Krenek T, Kovarik T, Alt V, Docheva D Bioeng Transl Med. 2022; 7(1):e10239.

PMID: 35079626 PMC: 8780039. DOI: 10.1002/btm2.10239.

MicroRNA-590-5p antagonizes the inhibitory effect of high glucose on osteoblast differentiation by suppressing Smad7 in MC3T3-E1 cells.

Chen Y, Sun C, Lu J, Zou L, Hu M, Yang Z J Int Med Res. 2019; 47(4):1740-1748.

PMID: 30803283 PMC: 6460606. DOI: 10.1177/0300060519830212.

References

Torii Y, Hitomi K, Yamagishi Y, Tsukagoshi N . Demonstration of alkaline phosphatase participation in the mineralization of osteoblasts by antisense RNA approach. Cell Biol Int. 1996; 20(7):459-64. DOI: 10.1006/cbir.1996.0060. View

Quirynen M, Abarca M, Van Assche N, Nevins M, van Steenberghe D . Impact of supportive periodontal therapy and implant surface roughness on implant outcome in patients with a history of periodontitis. J Clin Periodontol. 2007; 34(9):805-15. DOI: 10.1111/j.1600-051X.2007.01106.x. View

BARBER H, Seckinger R, Hayden R, Weinstein G . Evaluation of osseointegration of endosseous implants in radiated, vascularized fibula flaps to the mandible: a pilot study. J Oral Maxillofac Surg. 1995; 53(6):640-4; discussion 644-5. DOI: 10.1016/0278-2391(95)90158-2. View

Jeong H, Jin Y, Choi Y, Yum J, Choi J, Yeo C . PKC signaling inhibits osteogenic differentiation through the regulation of Msx2 function. Biochim Biophys Acta. 2012; 1823(8):1225-32. DOI: 10.1016/j.bbamcr.2012.05.018. View

Fiorino C, Harrison R . E-cadherin is important for cell differentiation during osteoclastogenesis. Bone. 2016; 86:106-18. DOI: 10.1016/j.bone.2016.03.004. View

Park J, Koak J, Jang J, Han C, Kim S, Heo S . Osseointegration of anodized titanium implants coated with fibroblast growth factor-fibronectin (FGF-FN) fusion protein. Int J Oral Maxillofac Implants. 2006; 21(6):859-66. View

Albrektsson T, Wennerberg A . The impact of oral implants - past and future, 1966-2042. J Can Dent Assoc. 2005; 71(5):327. View

Karsenty G, Kronenberg H, Settembre C . Genetic control of bone formation. Annu Rev Cell Dev Biol. 2009; 25:629-48. DOI: 10.1146/annurev.cellbio.042308.113308. View

Hansson H, Albrektsson T, Branemark P . Structural aspects of the interface between tissue and titanium implants. J Prosthet Dent. 1983; 50(1):108-13. DOI: 10.1016/0022-3913(83)90175-0. View

10.

Adell R, Lekholm U, Rockler B, Branemark P . A 15-year study of osseointegrated implants in the treatment of the edentulous jaw. Int J Oral Surg. 1981; 10(6):387-416. DOI: 10.1016/s0300-9785(81)80077-4. View

11.

Sergo V, Sbaizero O, Clarke D . Mechanical and chemical consequences of the residual stresses in plasma sprayed hydroxyapatite coatings. Biomaterials. 1997; 18(6):477-82. DOI: 10.1016/s0142-9612(96)00147-0. View

12.

Lee M, Lowe G, Strong D, Wergedal J, Glackin C . TWIST, a basic helix-loop-helix transcription factor, can regulate the human osteogenic lineage. J Cell Biochem. 1999; 75(4):566-77. DOI: 10.1002/(sici)1097-4644(19991215)75:4<566::aid-jcb3>3.0.co;2-0. View

13.

Bordji K, Jouzeau J, Mainard D, Payan E, Netter P, Rie K . Cytocompatibility of Ti-6Al-4V and Ti-5Al-2.5Fe alloys according to three surface treatments, using human fibroblasts and osteoblasts. Biomaterials. 1996; 17(9):929-40. DOI: 10.1016/0142-9612(96)83289-3. View

14.

Ong J, Chan D . Hydroxyapatite and their use as coatings in dental implants: a review. Crit Rev Biomed Eng. 2001; 28(5-6):667-707. DOI: 10.1615/critrevbiomedeng.v28.i56.10. View

15.

Yang Y, Kim K, Ong J . A review on calcium phosphate coatings produced using a sputtering process--an alternative to plasma spraying. Biomaterials. 2004; 26(3):327-37. DOI: 10.1016/j.biomaterials.2004.02.029. View

16.

Gorbahn M, Klein M, Lehnert M, Ziebart T, Brullmann D, Koper I . Promotion of osteogenic cell response using quasicovalent immobilized fibronectin on titanium surfaces: introduction of a novel biomimetic layer system. J Oral Maxillofac Surg. 2012; 70(8):1827-34. DOI: 10.1016/j.joms.2012.04.004. View

17.

Elias C, Oshida Y, Lima J, Muller C . Relationship between surface properties (roughness, wettability and morphology) of titanium and dental implant removal torque. J Mech Behav Biomed Mater. 2009; 1(3):234-42. DOI: 10.1016/j.jmbbm.2007.12.002. View

18.

Schwartz M, Assoian R . Integrins and cell proliferation: regulation of cyclin-dependent kinases via cytoplasmic signaling pathways. J Cell Sci. 2001; 114(Pt 14):2553-60. DOI: 10.1242/jcs.114.14.2553. View

19.

Mano T, Ishikawa K, Harada K, Umeda H, Ueyama Y . Comparison of apatite-coated titanium prepared by blast coating and flame spray methods--evaluation using simulated body fluid and initial histological study. Dent Mater J. 2011; 30(4):431-7. DOI: 10.4012/dmj.2010-162. View

20.

Mano T, Ueyama Y, Ishikawa K, Matsumura T, Suzuki K . Initial tissue response to a titanium implant coated with apatite at room temperature using a blast coating method. Biomaterials. 2002; 23(9):1931-6. DOI: 10.1016/s0142-9612(01)00319-2. View