Cell Sheets of Co-cultured Endothelial Progenitor Cells and Mesenchymal Stromal Cells Promote Osseointegration in Irradiated Rat Bone

Overview

Journal Sci Rep

Specialty Science

Date 2017 Jun 10

PMID 28596582

Citations 12

Authors

Huan Liu

Wei Zhou

Nan Ren

Zhihong Feng

Yan Dong

Shizhu Bai

Yang Jiao

Zhongshan Wang

Yimin Zhao

Affiliations

Soon will be listed here.

Abstract

Irradiated bone has a greater risk of implant failure than nonirradiated bone. The purpose of this study was to investigate the influence of cell sheets composed of co-cultured bone marrow mesenchymal stromal cells (BMSCs) and endothelial progenitor cells (EPCs) on implant osseointegration in irradiated bone. Cell sheets (EPCs, BMSCs or co-cultured EPCs and BMSCs) were wrapped around titanium implants to make cell sheet-implant complexes. The co-cultured group showed the highest osteogenic differentiation potential in vitro, as indicated by the extracellular matrix mineralization and the expression of osteogenesis related genes at both mRNA and protein levels. The co-cultured cells promoted ectopic bone formation as indicated by micro-computed tomography (Micro-CT) and histological analysis. In the irradiated tibias of rats, implants of the co-cultured group showed enhanced osseointegration by Micro-CT evaluation and histological observation. Co-cultured EPCs and BMSCs also up-regulated the expression of osteogenesis related genes in bone fragments in close contact with implants. In conclusion, cell sheets of co-cultured EPCs and BMSCs could promote osseous healing around implants and are potentially useful to improve osseointegration process for patients after radiotherapy.

Citing Articles

Bone Regeneration in Small and Large Segmental Bone Defect Models after Radiotherapy Using Injectable Polymer-Based Biodegradable Materials Containing Strontium-Doped Hydroxyapatite Particles.

Ehret C, Aid R, Paiva Dos Santos B, Rey S, Letourneur D, Amedee Vilamitjana J Int J Mol Sci. 2023; 24(6).

PMID: 36982504 PMC: 10049363. DOI: 10.3390/ijms24065429.

Implant-bone-interface: Reviewing the impact of titanium surface modifications on osteogenic processes in vitro and in vivo.

Stich T, Alagboso F, Krenek T, Kovarik T, Alt V, Docheva D Bioeng Transl Med. 2022; 7(1):e10239.

PMID: 35079626 PMC: 8780039. DOI: 10.1002/btm2.10239.

Survival of dental implants and occurrence of osteoradionecrosis in irradiated head and neck cancer patients: a systematic review and meta-analysis.

Toneatti D, Graf R, Burkhard J, Schaller B Clin Oral Investig. 2021; 25(10):5579-5593.

PMID: 34401944 PMC: 8443505. DOI: 10.1007/s00784-021-04065-6.

Co-culture with Endothelial Progenitor Cells promotes the Osteogenesis of Bone Mesenchymal Stem Cells via the VEGF-YAP axis in high-glucose environments.

Wu P, Zhang X, Hu Y, Liu D, Song J, Xu W Int J Med Sci. 2021; 18(7):1628-1638.

PMID: 33746579 PMC: 7976568. DOI: 10.7150/ijms.52316.

Endothelial progenitor cells with stem cells enhance osteogenic efficacy.

Li Q, Yu T, Wang F, Liu X, Wang Z Am J Transl Res. 2020; 12(6):2409-2424.

PMID: 32655780 PMC: 7344071.

References

Zigdon-Giladi H, Bick T, Lewinson D, Machtei E . Mesenchymal stem cells and endothelial progenitor cells stimulate bone regeneration and mineral density. J Periodontol. 2013; 85(7):984-90. DOI: 10.1902/jop.2013.130475. View

Li Q, Wang Z . Influence of mesenchymal stem cells with endothelial progenitor cells in co-culture on osteogenesis and angiogenesis: an in vitro study. Arch Med Res. 2013; 44(7):504-13. DOI: 10.1016/j.arcmed.2013.09.009. View

Villars F, Bordenave L, Bareille R, Amedee J . Effect of human endothelial cells on human bone marrow stromal cell phenotype: role of VEGF?. J Cell Biochem. 2000; 79(4):672-85. DOI: 10.1002/1097-4644(20001215)79:4<672::aid-jcb150>3.0.co;2-2. View

Fu W, Xiang Z, Huang F, Gu Z, Yu X, Cen S . Coculture of peripheral blood-derived mesenchymal stem cells and endothelial progenitor cells on strontium-doped calcium polyphosphate scaffolds to generate vascularized engineered bone. Tissue Eng Part A. 2014; 21(5-6):948-59. DOI: 10.1089/ten.TEA.2014.0267. View

Chen G, Qi Y, Niu L, Di T, Zhong J, Fang T . Application of the cell sheet technique in tissue engineering. Biomed Rep. 2015; 3(6):749-757. PMC: 4660585. DOI: 10.3892/br.2015.522. View

Matsumoto T, Kuroda R, Mifune Y, Kawamoto A, Shoji T, Miwa M . Circulating endothelial/skeletal progenitor cells for bone regeneration and healing. Bone. 2008; 43(3):434-9. DOI: 10.1016/j.bone.2008.05.001. View

Smadja D, Bieche I, Silvestre J, Germain S, Cornet A, Laurendeau I . Bone morphogenetic proteins 2 and 4 are selectively expressed by late outgrowth endothelial progenitor cells and promote neoangiogenesis. Arterioscler Thromb Vasc Biol. 2008; 28(12):2137-43. DOI: 10.1161/ATVBAHA.108.168815. View

Zhang L, Wu K, Song W, Xu H, An R, Zhao L . Chitosan/siCkip-1 biofunctionalized titanium implant for improved osseointegration in the osteoporotic condition. Sci Rep. 2015; 5:10860. PMC: 4455222. DOI: 10.1038/srep10860. View

Agarwal R, Garcia A . Biomaterial strategies for engineering implants for enhanced osseointegration and bone repair. Adv Drug Deliv Rev. 2015; 94:53-62. PMC: 4598264. DOI: 10.1016/j.addr.2015.03.013. View

10.

Zhou W, Han C, Song Y, Yan X, Li D, Chai Z . The performance of bone marrow mesenchymal stem cell--implant complexes prepared by cell sheet engineering techniques. Biomaterials. 2010; 31(12):3212-21. DOI: 10.1016/j.biomaterials.2010.01.036. View

11.

Raida M, Heymann A, Gunther C, Niederwieser D . Role of bone morphogenetic protein 2 in the crosstalk between endothelial progenitor cells and mesenchymal stem cells. Int J Mol Med. 2006; 18(4):735-9. DOI: 10.3892/ijmm.18.4.735. View

12.

Ziebart T, Schnell A, Walter C, Kammerer P, Pabst A, Lehmann K . Interactions between endothelial progenitor cells (EPC) and titanium implant surfaces. Clin Oral Investig. 2012; 17(1):301-9. DOI: 10.1007/s00784-012-0691-7. View

13.

Esposito M, Worthington H . Interventions for replacing missing teeth: hyperbaric oxygen therapy for irradiated patients who require dental implants. Cochrane Database Syst Rev. 2013; (9):CD003603. PMC: 6787936. DOI: 10.1002/14651858.CD003603.pub3. View

14.

Yu M, Zhou W, Song Y, Yu F, Li D, Na S . Development of mesenchymal stem cell-implant complexes by cultured cells sheet enhances osseointegration in type 2 diabetic rat model. Bone. 2011; 49(3):387-94. DOI: 10.1016/j.bone.2011.05.025. View

15.

Chambrone L, Mandia Jr J, Shibli J, Romito G, Abrahao M . Dental implants installed in irradiated jaws: a systematic review. J Dent Res. 2013; 92(12 Suppl):119S-30S. PMC: 3860067. DOI: 10.1177/0022034513504947. View

16.

Piras de Oliveira J, Abrahao M, Lauria Dib L . Extraoral implants in irradiated patients. Braz J Otorhinolaryngol. 2013; 79(2):185-9. PMC: 9443833. DOI: 10.5935/1808-8694.20130033. View

17.

Asahara T, Murohara T, Sullivan A, Silver M, van der Zee R, Li T . Isolation of putative progenitor endothelial cells for angiogenesis. Science. 1997; 275(5302):964-7. DOI: 10.1126/science.275.5302.964. View

18.

Yan J, Zhang C, Zhao Y, Cao C, Wu K, Zhao L . Non-viral oligonucleotide antimiR-138 delivery to mesenchymal stem cell sheets and the effect on osteogenesis. Biomaterials. 2014; 35(27):7734-49. DOI: 10.1016/j.biomaterials.2014.05.089. View

19.

Henrich D, Seebach C, Kaehling C, Scherzed A, Wilhelm K, Tewksbury R . Simultaneous cultivation of human endothelial-like differentiated precursor cells and human marrow stromal cells on beta-tricalcium phosphate. Tissue Eng Part C Methods. 2009; 15(4):551-60. DOI: 10.1089/ten.TEC.2008.0385. View

20.

Harrison J, Stratemann S, Redding S . Dental implants for patients who have had radiation treatment for head and neck cancer. Spec Care Dentist. 2004; 23(6):223-9. DOI: 10.1111/j.1754-4505.2003.tb00316.x. View