Characterization of the Gene from Sp. Strain PPE7 and Its Influence on Extracellular Polysaccharide Production and Virulence on

Overview

Journal Plant Pathol J

Date 2017 Jun 9

PMID 28592946

Authors

Min Keun Kim

Sun Mi Lee

Su Won Seuk

Jae San Ryu

Hee Dae Kim

Jin Hyeuk Kwon

Yong Jo Choi

Han Dae Yun

Affiliations

Soon will be listed here.

Abstract

RcsA is a positive activator of extracellular polysaccharide (EPS) synthesis in the . The gene of the soft rot pathogen sp. strain PPE7 in was cloned by PCR amplification, and its role in EPS synthesis and virulence was investigated. The RcsA protein contains 3 highly conserved domains, and the C-terminal end of the open reading frame shared significant amino acid homology to the helix-turn-helix DNA binding motif of bacterial activator proteins. The inactivation of by insertional mutagenesis created mutants that had decreased production of EPS compared to the wild-type strain and abolished the virulence of sp. strain PPE7 in . The sp. strain PPE7 gene was shown to strongly affect the formation of the disease symptoms of a mushroom pathogen and to act as the virulence factor to cause soft rot disease in .

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