Raman Spectroscopy As a Tool for Ecology and Evolution
Overview
Biomedical Engineering
Biophysics
Authors
Affiliations
Scientists are always on the lookout for new modalities of information which could reveal new biological features that are useful for deciphering the complexity of biological systems. Here, we introduce Raman spectroscopy as a prime candidate for ecology and evolution. To encourage the integration of this microscopy technique in the field of ecology and evolution, it is crucial to discuss first how Raman spectroscopy fits within the conceptual, technical and pragmatic considerations of ecology and evolution. In this paper, we show that the spectral information holds reliable indicators of intra- and interspecies variations, which can be related to the environment, selective pressures and fitness. Moreover, we show how the technical and pragmatic aspects of this modality (non-destructive, non-labelling, speed, relative low cost, etc.) enable it to be combined with more conventional methodologies. With this paper, we hope to open new avenues of research and extend the scope of available methodologies used in ecology and evolution.
Vibrational spectroscopic detection of radiation-induced structural changes in hemoglobin.
Gaikwad P, Hole A, Saxena V, Choudhury S, Nath B, Murali Krishna C Biochem Biophys Rep. 2024; 38:101721.
PMID: 38766383 PMC: 11101703. DOI: 10.1016/j.bbrep.2024.101721.
Chen H, Wang X, Li C, Xu X, Wang G Arch Microbiol. 2024; 206(5):227.
PMID: 38642141 DOI: 10.1007/s00203-024-03941-5.
Berneschi S, DAndrea C, Baldini F, Banchelli M, de Angelis M, Pelli S Anal Bioanal Chem. 2021; 413(24):6171-6182.
PMID: 34278523 DOI: 10.1007/s00216-021-03418-0.
Korinth F, Mondol A, Stiebing C, Schie I, Krafft C, Popp J Sci Rep. 2020; 10(1):11215.
PMID: 32641779 PMC: 7343813. DOI: 10.1038/s41598-020-67897-4.
Effect of Laser Irradiation on Cell Function and Its Implications in Raman Spectroscopy.
Yuan X, Song Y, Song Y, Xu J, Wu Y, Glidle A Appl Environ Microbiol. 2018; 84(8).
PMID: 29427427 PMC: 5881070. DOI: 10.1128/AEM.02508-17.