Selection of Human Blastocysts with a High Implantation Potential Based on Timely Compaction

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2017 Jun 3

PMID 28573523

Citations 7

Authors

Yamato Mizobe

Yuji Ezono

Makoto Tokunaga

Naoto Oya

Reiko Iwakiri

Naomi Yoshida

Yumi Sato

Nanase Onoue

Kazuchika Miyoshi

Affiliations

Soon will be listed here.

Abstract

Purpose: Recently, we established a noninvasive system for selecting human blastocysts with a high pre-transfer implantation potential based on first and second division patterns. The present study was carried out to improve the selection system.

Methods: Embryos that completed first and second divisions within 25.90 and 37.88 h after culture, respectively, were selected using a time-lapse incubator. We examined the effects of compaction and blastocyst formation times on pregnancy rates after transferring these embryos at the blastocyst stage.

Results: The completion of compaction and blastocyst formation times (79.93 and 97.47 h after culture, respectively) of embryos resulting in pregnancies after transfer were significantly (P < 0.01) shorter than those (86.46 and 100.34 h after culture, respectively) of embryos that failed to induce pregnancies. Embryo selection based on completion of compaction time improved pregnancy rates (40.9 vs. 74.6%, P < 0.01).

Conclusions: Of the embryos that formed two cells during the first division within 25.90 h after culture and four cells during the second division within 37.88 h after culture, those that completed compaction within 79.93 h after culture before reaching the blastocyst stage had a high implantation potential.

Citing Articles

Effect of day 3 embryo cell number on the pregnancy and neonatal outcomes of day 4 single embryo transfer from fresh cycles.

Tao L, Zheng B, Li G, Geng Y, Guo Y, Dai H BMC Pregnancy Childbirth. 2024; 24(1):775.

PMID: 39580384 PMC: 11585943. DOI: 10.1186/s12884-024-06976-0.

The morphokinetic signature of human blastocysts with mosaicism and the clinical outcomes following transfer of embryos with low-level mosaicism.

Zou Y, Sui Y, Fu J, Ge N, Sun X, Sun Y J Ovarian Res. 2024; 17(1):10.

PMID: 38195558 PMC: 10775590. DOI: 10.1186/s13048-023-01324-w.

Early Compaction Might Be a Parameter to Determine Good Quality Embryos and Day of Embryo Transfer in Patients Undergoing Intracytoplasmic Sperm Injection.

Ozturk S, Cincik M, Donmez Cakil Y, Sayan S, Selam B Cureus. 2022; 14(3):e23593.

PMID: 35494986 PMC: 9046904. DOI: 10.7759/cureus.23593.

Effects of oxygen tension and humidity on the preimplantation development of mouse embryos produced by in vitro fertilization: analysis using a non-humidifying incubator with time-lapse cinematography.

Watanabe H, Ito H, Shintome A, Suzuki H Exp Anim. 2022; 71(3):338-346.

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Mechanisms of human embryo development: from cell fate to tissue shape and back.

Shahbazi M Development. 2020; 147(14).

PMID: 32680920 PMC: 7375473. DOI: 10.1242/dev.190629.

References

Tao J, Tamis R, Fink K, Williams B, Craig R . The neglected morula/compact stage embryo transfer. Hum Reprod. 2002; 17(6):1513-8. DOI: 10.1093/humrep/17.6.1513. View

Fleming T, Sheth B, Fesenko I . Cell adhesion in the preimplantation mammalian embryo and its role in trophectoderm differentiation and blastocyst morphogenesis. Front Biosci. 2001; 6:D1000-7. DOI: 10.2741/fleming. View

Nikas G, Ao A, Winston R, Handyside A . Compaction and surface polarity in the human embryo in vitro. Biol Reprod. 1996; 55(1):32-7. DOI: 10.1095/biolreprod55.1.32. View

Kirkegaard K, Kesmodel U, Hindkjaer J, Ingerslev H . Time-lapse parameters as predictors of blastocyst development and pregnancy outcome in embryos from good prognosis patients: a prospective cohort study. Hum Reprod. 2013; 28(10):2643-51. DOI: 10.1093/humrep/det300. View

Goldman R, Kaser D, Missmer S, Srouji S, Farland L, Racowsky C . Building a model to increase live birth rate through patient-specific optimization of embryo transfer day. J Assist Reprod Genet. 2016; 33(11):1525-1532. PMC: 5125155. DOI: 10.1007/s10815-016-0803-4. View

Cruz M, Garrido N, Herrero J, Perez-Cano I, Munoz M, Meseguer M . Timing of cell division in human cleavage-stage embryos is linked with blastocyst formation and quality. Reprod Biomed Online. 2012; 25(4):371-81. DOI: 10.1016/j.rbmo.2012.06.017. View

Fenwick J, Platteau P, Murdoch A, Herbert M . Time from insemination to first cleavage predicts developmental competence of human preimplantation embryos in vitro. Hum Reprod. 2002; 17(2):407-12. DOI: 10.1093/humrep/17.2.407. View

Giorgetti C, Hans E, Terriou P, Salzmann J, Barry B, Chabert-Orsini V . Early cleavage: an additional predictor of high implantation rate following elective single embryo transfer. Reprod Biomed Online. 2007; 14(1):85-91. DOI: 10.1016/s1472-6483(10)60768-7. View

Lee M, Lee R, Lin M, Hwu Y . Cleavage speed and implantation potential of early-cleavage embryos in IVF or ICSI cycles. J Assist Reprod Genet. 2012; 29(8):745-50. PMC: 3430780. DOI: 10.1007/s10815-012-9777-z. View

10.

Kuwayama M, Vajta G, Kato O, Leibo S . Highly efficient vitrification method for cryopreservation of human oocytes. Reprod Biomed Online. 2005; 11(3):300-8. DOI: 10.1016/s1472-6483(10)60837-1. View

11.

Lemmen J, Agerholm I, Ziebe S . Kinetic markers of human embryo quality using time-lapse recordings of IVF/ICSI-fertilized oocytes. Reprod Biomed Online. 2008; 17(3):385-91. DOI: 10.1016/s1472-6483(10)60222-2. View

12.

Kirkegaard K, Sundvall L, Erlandsen M, Hindkjaer J, Knudsen U, Ingerslev H . Timing of human preimplantation embryonic development is confounded by embryo origin. Hum Reprod. 2015; 31(2):324-31. PMC: 4716807. DOI: 10.1093/humrep/dev296. View

13.

Dennis S, Thomas M, Williams D, Robins J . Embryo morphology score on day 3 is predictive of implantation and live birth rates. J Assist Reprod Genet. 2006; 23(4):171-5. PMC: 3454961. DOI: 10.1007/s10815-006-9027-3. View

14.

Cruz M, Gadea B, Garrido N, Pedersen K, Martinez M, Perez-Cano I . Embryo quality, blastocyst and ongoing pregnancy rates in oocyte donation patients whose embryos were monitored by time-lapse imaging. J Assist Reprod Genet. 2011; 28(7):569-73. PMC: 3162049. DOI: 10.1007/s10815-011-9549-1. View

15.

Rubio I, Galan A, Larreategui Z, Ayerdi F, Bellver J, Herrero J . Clinical validation of embryo culture and selection by morphokinetic analysis: a randomized, controlled trial of the EmbryoScope. Fertil Steril. 2014; 102(5):1287-1294.e5. DOI: 10.1016/j.fertnstert.2014.07.738. View

16.

Emiliani S, Fasano G, Vandamme B, Vannin A, Verdoodt M, Biramane J . Impact of the assessment of early cleavage in a single embryo transfer policy. Reprod Biomed Online. 2006; 13(2):255-60. DOI: 10.1016/s1472-6483(10)60623-2. View

17.

Kuwayama M . Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology. 2006; 67(1):73-80. DOI: 10.1016/j.theriogenology.2006.09.014. View

18.

Skiadas C, Jackson K, Racowsky C . Early compaction on day 3 may be associated with increased implantation potential. Fertil Steril. 2006; 86(5):1386-91. DOI: 10.1016/j.fertnstert.2006.03.051. View

19.

Conaghan J, Chen A, Willman S, Ivani K, Chenette P, Boostanfar R . Improving embryo selection using a computer-automated time-lapse image analysis test plus day 3 morphology: results from a prospective multicenter trial. Fertil Steril. 2013; 100(2):412-9.e5. DOI: 10.1016/j.fertnstert.2013.04.021. View

20.

Pinborg A, Loft A, Rasmussen S, Schmidt L, Langhoff-Roos J, Greisen G . Neonatal outcome in a Danish national cohort of 3438 IVF/ICSI and 10,362 non-IVF/ICSI twins born between 1995 and 2000. Hum Reprod. 2004; 19(2):435-41. DOI: 10.1093/humrep/deh063. View