An Integrated Analysis Revealed Different MicroRNA-mRNA Profiles During Skeletal Muscle Development Between Landrace and Lantang Pigs

Overview

Journal Sci Rep

Specialty Science

Date 2017 Jun 2

PMID 28566753

Citations 5

Authors

Shuihua Xie

Luxi Chen

Xumeng Zhang

Xiaohong Liu

Yaosheng Chen

Delin Mo

Affiliations

Soon will be listed here.

Abstract

Pigs supply vital dietary proteins for human consumption, and their economic value depends largely on muscle production. MicroRNAs are known to play important roles in skeletal muscle development. However, their relationship to distinct muscle production between pig breeds remains unknown. Here, we performed an integrated analysis of microRNA-mRNA expression profiles for Landrace (LR, lean) pigs and the Chinese indigenous Lantang pig (LT, lard-type) during 8 stages of skeletal muscle developmental, including at 35, 49, 63, 77 dpc (days post coitum) and 2, 28, 90, 180 dpn (days postnatal). As differentially expressed-miRNA expression profiles can be well classified into two clusters by PCA analysis, we grouped the embryonic stages as G1 and the postnatal stages as G2. A total of 203 genes were predicted miRNA targets, and a STEM analysis showed distinct expression patterns between G1 and G2 in both breeds based on their transcriptomic data. Furthermore, a STRING analysis predicted interactions between 22 genes and 35 miRNAs, including some crucial myogenic factors and myofibrillar genes. Thus, it can be reasonably speculated that myogenic miRNAs may regulate myofibrillar genes in myofiber formation during embryonic stages and muscle hypertrophy during postnatal stages, leading to distinct differences in muscle production between breeds.

Citing Articles

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References

Zhao X, Mo D, Li A, Gong W, Xiao S, Zhang Y . Comparative analyses by sequencing of transcriptomes during skeletal muscle development between pig breeds differing in muscle growth rate and fatness. PLoS One. 2011; 6(5):e19774. PMC: 3102668. DOI: 10.1371/journal.pone.0019774. View

Donati C, Marseglia G, Magi A, Serrati S, Cencetti F, Bernacchioni C . Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for GATA6. PLoS One. 2011; 6(5):e20389. PMC: 3101247. DOI: 10.1371/journal.pone.0020389. View

Charge S, Rudnicki M . Cellular and molecular regulation of muscle regeneration. Physiol Rev. 2004; 84(1):209-38. DOI: 10.1152/physrev.00019.2003. View

Siengdee P, Trakooljul N, Murani E, Brand B, Schwerin M, Wimmers K . Pre- and post-natal muscle microRNA expression profiles of two pig breeds differing in muscularity. Gene. 2015; 561(2):190-8. DOI: 10.1016/j.gene.2015.02.035. View

Kim H, Lee Y, Sivaprasad U, Malhotra A, Dutta A . Muscle-specific microRNA miR-206 promotes muscle differentiation. J Cell Biol. 2006; 174(5):677-87. PMC: 2064311. DOI: 10.1083/jcb.200603008. View

Glass D . Skeletal muscle hypertrophy and atrophy signaling pathways. Int J Biochem Cell Biol. 2005; 37(10):1974-84. DOI: 10.1016/j.biocel.2005.04.018. View

Tang Z, Li Y, Wan P, Li X, Zhao S, Liu B . LongSAGE analysis of skeletal muscle at three prenatal stages in Tongcheng and Landrace pigs. Genome Biol. 2007; 8(6):R115. PMC: 2394763. DOI: 10.1186/gb-2007-8-6-r115. View

Qin L, Chen Y, Liu X, Ye S, Yu K, Huang Z . Integrative analysis of porcine microRNAome during skeletal muscle development. PLoS One. 2013; 8(9):e72418. PMC: 3770649. DOI: 10.1371/journal.pone.0072418. View

Rau F, Laine J, Ramanoudjame L, Ferry A, Arandel L, Delalande O . Abnormal splicing switch of DMD's penultimate exon compromises muscle fibre maintenance in myotonic dystrophy. Nat Commun. 2015; 6:7205. PMC: 4458869. DOI: 10.1038/ncomms8205. View

10.

Liu N, Bassel-Duby R . Regulation of skeletal muscle development and disease by microRNAs. Results Probl Cell Differ. 2014; 56:165-90. DOI: 10.1007/978-3-662-44608-9_8. View

11.

Kemp T, Sadusky T, Simon M, Brown R, Eastwood M, Sassoon D . Identification of a novel stretch-responsive skeletal muscle gene (Smpx). Genomics. 2001; 72(3):260-71. DOI: 10.1006/geno.2000.6461. View

12.

Genovesi L, Carter K, Gottardo N, Giles K, Dallas P . Integrated analysis of miRNA and mRNA expression in childhood medulloblastoma compared with neural stem cells. PLoS One. 2011; 6(9):e23935. PMC: 3170291. DOI: 10.1371/journal.pone.0023935. View

13.

Zhou L, Wang L, Lu L, Jiang P, Sun H, Wang H . Inhibition of miR-29 by TGF-beta-Smad3 signaling through dual mechanisms promotes transdifferentiation of mouse myoblasts into myofibroblasts. PLoS One. 2012; 7(3):e33766. PMC: 3306299. DOI: 10.1371/journal.pone.0033766. View

14.

Suzuki A, Kojima N, Ikeuchi Y, Ikarashi S, Moriyama N, Ishizuka T . Carcass composition and meat quality of Chinese purebred and European × Chinese crossbred pigs. Meat Sci. 2011; 29(1):31-41. DOI: 10.1016/0309-1740(91)90021-H. View

15.

Nelson P, Kiriakidou M, Sharma A, Maniataki E, Mourelatos Z . The microRNA world: small is mighty. Trends Biochem Sci. 2003; 28(10):534-40. DOI: 10.1016/j.tibs.2003.08.005. View

16.

Zhang X, Chen Y, Pan J, Liu X, Chen H, Zhou X . iTRAQ-based quantitative proteomic analysis reveals the distinct early embryo myofiber type characteristics involved in landrace and miniature pig. BMC Genomics. 2016; 17:137. PMC: 4766617. DOI: 10.1186/s12864-016-2464-1. View

17.

Wan L, Ma J, Xu G, Wang D, Wang N . Molecular cloning, structural analysis and tissue expression of protein phosphatase 3 catalytic subunit alpha isoform (PPP3CA) gene in Tianfu goat muscle. Int J Mol Sci. 2014; 15(2):2346-58. PMC: 3958854. DOI: 10.3390/ijms15022346. View

18.

Kloosterman W, Plasterk R . The diverse functions of microRNAs in animal development and disease. Dev Cell. 2006; 11(4):441-50. DOI: 10.1016/j.devcel.2006.09.009. View

19.

Schubert W, Sotgia F, Cohen A, Capozza F, Bonuccelli G, Bruno C . Caveolin-1(-/-)- and caveolin-2(-/-)-deficient mice both display numerous skeletal muscle abnormalities, with tubular aggregate formation. Am J Pathol. 2007; 170(1):316-33. PMC: 1762679. DOI: 10.2353/ajpath.2007.060687. View

20.

Koutsoulidou A, Mastroyiannopoulos N, Furling D, Uney J, Phylactou L . Expression of miR-1, miR-133a, miR-133b and miR-206 increases during development of human skeletal muscle. BMC Dev Biol. 2011; 11:34. PMC: 3132729. DOI: 10.1186/1471-213X-11-34. View