T2 Magnetic Resonance Assay-Based Direct Detection of Three Lyme Disease-Related Borrelia Species in Whole-Blood Samples

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2017 Jun 2

PMID 28566314

Citations 5

Authors

Jessica L Snyder

Heidi Giese

Cheryl Bandoski-Gralinski

Jessica Townsend

Beck E Jacobson

Robert Shivers

Anna M Schotthoefer

Thomas R Fritsche

Clayton Green

Steven M Callister

John A Branda

Thomas J Lowery

Affiliations

Soon will be listed here.

Abstract

In early Lyme disease (LD), serologic testing is insensitive and seroreactivity may reflect active or past infection. In this study, we evaluated a novel assay for the direct detection of three species of spirochetes in whole blood. The T2 magnetic resonance (T2MR) assay platform was used to amplify DNA released from intact spirochetes and to detect amplicon. Analytical sensitivity was determined from blood spiked with known concentrations of spirochetes, and the assay's limit of detection was found to be in the single-cell-per-milliliter range: 5 cells/ml for and 8 cells/ml for and Clinical samples ( = 66) from confirmed or suspected early LD patients were also analyzed. was detected using T2MR in 2/2 (100%) of blood samples from patients with confirmed early LD, based on the presence of erythema migrans and documentation of seroconversion or a positive real-time blood PCR. T2MR detected in blood samples from 17/54 (31%) of patients with probable LD, based on the presence of erythema migrans without documented seroconversion or of documented seroconversion in patients with a compatible clinical syndrome but without erythema migrans. Out of 21 clinical samples tested by real-time PCR, only 1 was positive and 13 were negative with agreement with T2MR. An additional 7 samples that were negative by real-time PCR were positive with T2MR. Therefore, T2MR enables a low limit of detection (LoD) for spp. in whole blood samples and is able to detect in clinical samples.

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References

Collares-Pereira M, Couceiro S, Franca I, Kurtenbach K, Schafer S, Vitorino L . First isolation of Borrelia lusitaniae from a human patient. J Clin Microbiol. 2004; 42(3):1316-8. PMC: 356816. DOI: 10.1128/JCM.42.3.1316-1318.2004. View

Pritt B, Mead P, Hoang Johnson D, Neitzel D, Respicio-Kingry L, Davis J . Identification of a novel pathogenic Borrelia species causing Lyme borreliosis with unusually high spirochaetaemia: a descriptive study. Lancet Infect Dis. 2016; 16(5):556-564. PMC: 4975683. DOI: 10.1016/S1473-3099(15)00464-8. View

Priem S, Rittig M, Kamradt T, Burmester G, Krause A . An optimized PCR leads to rapid and highly sensitive detection of Borrelia burgdorferi in patients with Lyme borreliosis. J Clin Microbiol. 1997; 35(3):685-90. PMC: 229651. DOI: 10.1128/jcm.35.3.685-690.1997. View

Bratton R, Whiteside J, Hovan M, Engle R, Edwards F . Diagnosis and treatment of Lyme disease. Mayo Clin Proc. 2008; 83(5):566-71. DOI: 10.4065/83.5.566. View

. Recommendations for test performance and interpretation from the Second National Conference on Serologic Diagnosis of Lyme Disease. MMWR Morb Mortal Wkly Rep. 1995; 44(31):590-1. View

Goodman J, BRADLEY J, Ross A, Goellner P, Lagus A, Vitale B . Bloodstream invasion in early Lyme disease: results from a prospective, controlled, blinded study using the polymerase chain reaction. Am J Med. 1995; 99(1):6-12. DOI: 10.1016/s0002-9343(99)80097-7. View

Hilton E, DeVoti J, Benach J, Halluska M, White D, Paxton H . Seroprevalence and seroconversion for tick-borne diseases in a high-risk population in the northeast United States. Am J Med. 1999; 106(4):404-9. DOI: 10.1016/s0002-9343(99)00046-7. View

Hinckley A, Connally N, Meek J, Johnson B, Kemperman M, Feldman K . Lyme disease testing by large commercial laboratories in the United States. Clin Infect Dis. 2014; 59(5):676-81. PMC: 4646413. DOI: 10.1093/cid/ciu397. View

Pfaller M, Wolk D, Lowery T . T2MR and T2Candida: novel technology for the rapid diagnosis of candidemia and invasive candidiasis. Future Microbiol. 2015; 11(1):103-17. DOI: 10.2217/fmb.15.111. View

10.

Nowakowski J, McKenna D, Nadelman R, Bittker S, Cooper D, Pavia C . Blood cultures for patients with extracutaneous manifestations of Lyme disease in the United States. Clin Infect Dis. 2009; 49(11):1733-5. DOI: 10.1086/648076. View

11.

Bakken L, Callister S, Wand P, Schell R . Interlaboratory comparison of test results for detection of Lyme disease by 516 participants in the Wisconsin State Laboratory of Hygiene/College of American Pathologists Proficiency Testing Program. J Clin Microbiol. 1997; 35(3):537-43. PMC: 229622. DOI: 10.1128/jcm.35.3.537-543.1997. View

12.

Branda J, Strle F, Strle K, Sikand N, Ferraro M, Steere A . Performance of United States serologic assays in the diagnosis of Lyme borreliosis acquired in Europe. Clin Infect Dis. 2013; 57(3):333-40. PMC: 8210822. DOI: 10.1093/cid/cit235. View

13.

Wormser G, Dattwyler R, Shapiro E, Halperin J, Steere A, Klempner M . The clinical assessment, treatment, and prevention of lyme disease, human granulocytic anaplasmosis, and babesiosis: clinical practice guidelines by the Infectious Diseases Society of America. Clin Infect Dis. 2006; 43(9):1089-134. DOI: 10.1086/508667. View

14.

Kuehn B . CDC estimates 300,000 US cases of Lyme disease annually. JAMA. 2013; 310(11):1110. DOI: 10.1001/jama.2013.278331. View

15.

Borchers A, Keen C, Huntley A, Gershwin M . Lyme disease: a rigorous review of diagnostic criteria and treatment. J Autoimmun. 2014; 57:82-115. DOI: 10.1016/j.jaut.2014.09.004. View

16.

Markowicz M, Ladstatter S, Schotta A, Reiter M, Pomberger G, Stanek G . Oligoarthritis caused by Borrelia bavariensis, Austria, 2014. Emerg Infect Dis. 2015; 21(6):1052-4. PMC: 4451909. DOI: 10.3201/eid2106.141516. View

17.

Courtney J, Kostelnik L, Zeidner N, Massung R . Multiplex real-time PCR for detection of anaplasma phagocytophilum and Borrelia burgdorferi. J Clin Microbiol. 2004; 42(7):3164-8. PMC: 446246. DOI: 10.1128/JCM.42.7.3164-3168.2004. View

18.

Robertson J, Guy E, Andrews N, Wilske B, Anda P, Granstrom M . A European multicenter study of immunoblotting in serodiagnosis of lyme borreliosis. J Clin Microbiol. 2000; 38(6):2097-102. PMC: 86736. DOI: 10.1128/JCM.38.6.2097-2102.2000. View

19.

Lee S, Vigliotti V, Vigliotti J, Jones W, Pappu S . Increased sensitivity and specificity of Borrelia burgdorferi 16S ribosomal DNA detection. Am J Clin Pathol. 2010; 133(4):569-76. DOI: 10.1309/AJCPI72YAXRHYHEE. View

20.

Eshoo M, Crowder C, Rebman A, Rounds M, Matthews H, Picuri J . Direct molecular detection and genotyping of Borrelia burgdorferi from whole blood of patients with early Lyme disease. PLoS One. 2012; 7(5):e36825. PMC: 3348129. DOI: 10.1371/journal.pone.0036825. View