Chemical Composition and Antimicrobial Activities of Iranian Propolis

Overview

Journal Iran Biomed J

Specialties Biology
General Medicine

Date 2017 Jun 1

PMID 28558440

Citations 20

Authors

Houshang Afrouzan

Azar Tahghighi

Sedigheh Zakeri

Ali Es-Haghi

Affiliations

Soon will be listed here.

Abstract

Background: With considering the importance of natural products for their remedial and therapeutic value, this research was aimed to analyze the chemical compositions and antimicrobial activity of four propolis samples from different areas of Iran (Chenaran, Taleghan, Morad Beyg, and Kalaleh) with various climates and flora.

Methods: Ethanolic (70% EtOH) and dichlromethane (DCM) extracts of Iranian propolis were analyzed by gas chromatography-mass spectrometry (GC-MS) methods, and antimicrobial activity was evaluated against Candida albicans, Escherichia coli, and Staphylococcus aureus using disk diffusion antimicrobial method.

Results: The results of GC-MS analysis showed the presence of fatty acids, flavonoids, terpenes, aromatic-aliphatic acids, and their related esters. The total flavonoids in DCM extract of Chenaran, Taleghan, Morad Beyg, and Kalaleh propolis were pinocembrin and pinostrobin chalcone. The common phenolic and terpene compounds detected in all four tested EtOH extracts were P-cumaric acid and dimethyl -1,3,5,6-tetramethyl-[1,3-(13C2)] bicycloce [5.5.0] dodeca-1,3,5,6,8,10-hexaene-9,10-dicarboxylate, respectively. The highest inhibitory diameter zone of the Iranian propolis against C. albicans, E. coli, and S. aureus was for DCM extract of Kalaleh propolis (13.33 mm), Morad Beyg propolis (12 mm), and Kalaleh (11.67 mm), respectively.

Conclusion: Iranian propolis showed antimicrobial activities against C. albicans, E. coli, and S. aurous, perhaps due to the presence of flavonoids, phenolic acids, and terpenes as active components that can be used alone or in combination with the selected antibiotics to synergize antibiotic effect, as well as to prevent microbial resistance to available antimicrobial drugs.

Citing Articles

Hydrogel Containing Propolis: Physical Characterization and Evaluation of Biological Activities for Potential Use in the Treatment of Skin Lesions.

de Meneses Costa Ferreira L, Cruz N, Lynch D, Costa P, Salgado C, Silva-Junior J Pharmaceuticals (Basel). 2024; 17(10).

PMID: 39459039 PMC: 11510207. DOI: 10.3390/ph17101400.

Correlation between total phenolic and flavonoid contents and biological activities of 12 ethanolic extracts of Iranian propolis.

Fathi Hafshejani S, Lotfi S, Rezvannejad E, Mortazavi M, Riahi-Madvar A Food Sci Nutr. 2023; 11(7):4308-4325.

PMID: 37457164 PMC: 10345684. DOI: 10.1002/fsn3.3356.

Effects of Propolis Impregnation on Polylactic Acid (PLA) Scaffolds Loaded with Wollastonite Particles against , , and Their Coculture for Potential Medical Devices.

Moreno A, Orozco Y, Ocampo S, Malagon S, Ossa A, Pelaez-Vargas A Polymers (Basel). 2023; 15(12).

PMID: 37376275 PMC: 10301083. DOI: 10.3390/polym15122629.

Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis.

Queiroga M, Laranjo M, Andrade N, Marques M, Costa A, Antunes C Antibiotics (Basel). 2023; 12(2).

PMID: 36830258 PMC: 9952062. DOI: 10.3390/antibiotics12020347.

A Comprehensive Review of Natural Compounds for Wound Healing: Targeting Bioactivity Perspective.

Trinh X, Long N, Van Anh L, Nga P, Giang N, Chien P Int J Mol Sci. 2022; 23(17).

PMID: 36076971 PMC: 9455684. DOI: 10.3390/ijms23179573.

References

Sawaya A, Palma A, Caetano F, Marcucci M, da Silva Cunha I, Araujo C . Comparative study of in vitro methods used to analyse the activity of propolis extracts with different compositions against species of Candida. Lett Appl Microbiol. 2002; 35(3):203-7. DOI: 10.1046/j.1472-765x.2002.01169.x. View

Da Silva Filho A, Resende D, Fukui M, Santos F, Pauletti P, Cunha W . In vitro antileishmanial, antiplasmodial and cytotoxic activities of phenolics and triterpenoids from Baccharis dracunculifolia D. C. (Asteraceae). Fitoterapia. 2009; 80(8):478-82. DOI: 10.1016/j.fitote.2009.06.007. View

Mirzoeva O, Grishanin R, Calder P . Antimicrobial action of propolis and some of its components: the effects on growth, membrane potential and motility of bacteria. Microbiol Res. 1997; 152(3):239-46. DOI: 10.1016/S0944-5013(97)80034-1. View

Kartal M, Yildiz S, Kaya S, Kurucu S, Topcu G . Antimicrobial activity of propolis samples from two different regions of Anatolia. J Ethnopharmacol. 2003; 86(1):69-73. DOI: 10.1016/s0378-8741(03)00042-4. View

Machado G, Leon L, de Castro S . Activity of Brazilian and Bulgarian propolis against different species of Leishmania. Mem Inst Oswaldo Cruz. 2007; 102(1):73-7. DOI: 10.1590/s0074-02762007000100012. View

Chirumbolo S . Anti-inflammatory property of propolis. J Clin Biochem Nutr. 2015; 56(2):163-4. PMC: 4345176. DOI: 10.3164/jcbn.14-110. View

Silici S, Koc N, Ayangil D, Cankaya S . Antifungal activities of propolis collected by different races of honeybees against yeasts isolated from patients with superficial mycoses. J Pharmacol Sci. 2005; 99(1):39-44. DOI: 10.1254/jphs.fpe05002x. View

Banskota A, Tezuka Y, Kadota S . Recent progress in pharmacological research of propolis. Phytother Res. 2001; 15(7):561-71. DOI: 10.1002/ptr.1029. View

Ota C, Unterkircher C, Fantinato V, Shimizu M . Antifungal activity of propolis on different species of Candida. Mycoses. 2002; 44(9-10):375-8. DOI: 10.1046/j.1439-0507.2001.00671.x. View

10.

Velikova M, Bankova V, Sorkun K, Houcine S, Tsvetkova I, Kujumgiev A . Propolis from the Mediterranean region: chemical composition and antimicrobial activity. Z Naturforsch C J Biosci. 2000; 55(9-10):790-3. DOI: 10.1515/znc-2000-9-1019. View

11.

Kartal M, Kaya S, Kurucu S . GC-MS analysis of propolis samples from two different regions of Turkey. Z Naturforsch C J Biosci. 2002; 57(9-10):905-9. DOI: 10.1515/znc-2002-9-1025. View

12.

Duran N, Muz M, Culha G, Duran G, Ozer B . GC-MS analysis and antileishmanial activities of two Turkish propolis types. Parasitol Res. 2010; 108(1):95-105. DOI: 10.1007/s00436-010-2039-z. View

13.

Miguel M, Nunes S, Dandlen S, Cavaco A, Antunes M . Phenols and antioxidant activity of hydro-alcoholic extracts of propolis from Algarve, South of Portugal. Food Chem Toxicol. 2010; 48(12):3418-23. DOI: 10.1016/j.fct.2010.09.014. View

14.

BAUER A, KIRBY W, SHERRIS J, Turck M . Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45(4):493-6. View

15.

Uzel A, Sorkun K, Oncag O, Cogulu D, Gencay O, Salih B . Chemical compositions and antimicrobial activities of four different Anatolian propolis samples. Microbiol Res. 2005; 160(2):189-95. DOI: 10.1016/j.micres.2005.01.002. View

16.

Krol W, Scheller S, Shani J, Pietsz G, Czuba Z . Synergistic effect of ethanolic extract of propolis and antibiotics on the growth of staphylococcus aureus. Arzneimittelforschung. 1993; 43(5):607-9. View

17.

Isla M, Paredes-Guzman J, Nieva-Moreno M, Koo H, Park Y . Some chemical composition and biological activity of northern Argentine propolis. J Agric Food Chem. 2005; 53(4):1166-72. DOI: 10.1021/jf040130h. View

18.

Falcao S, Vale N, Cos P, Gomes P, Freire C, Maes L . In vitro evaluation of Portuguese propolis and floral sources for antiprotozoal, antibacterial and antifungal activity. Phytother Res. 2013; 28(3):437-43. DOI: 10.1002/ptr.5013. View

19.

Cushnie T, Lamb A . Antimicrobial activity of flavonoids. Int J Antimicrob Agents. 2005; 26(5):343-56. PMC: 7127073. DOI: 10.1016/j.ijantimicag.2005.09.002. View

20.

Raghukumar R, Vali L, Watson D, Fearnley J, Seidel V . Antimethicillin-resistant Staphylococcus aureus (MRSA) activity of 'pacific propolis' and isolated prenylflavanones. Phytother Res. 2010; 24(8):1181-7. DOI: 10.1002/ptr.3096. View