Co-infecting Microorganisms Dramatically Alter Pathogen Gene Essentiality During Polymicrobial Infection

Overview

Journal Nat Microbiol

Specialties Microbiology
Parasitology

Date 2017 May 31

PMID 28555625

Citations 59

Authors

Carolyn B Ibberson

Apollo Stacy

Derek Fleming

Justine L Dees

Kendra Rumbaugh

Michael S Gilmore

Marvin Whiteley

Affiliations

Soon will be listed here.

Abstract

Identifying genes required by pathogens during infection is critical for antimicrobial development. Here, we use a Monte Carlo simulation-based method to analyse high-throughput transposon sequencing data to determine the role of infection site and co-infecting microorganisms on the in vivo 'essential' genome of Staphylococcus aureus. We discovered that co-infection of murine surgical wounds with Pseudomonas aeruginosa results in conversion of ∼25% of the in vivo S. aureus mono-culture essential genes to non-essential. Furthermore, 182 S. aureus genes are uniquely essential during co-infection. These 'community dependent essential' (CoDE) genes illustrate the importance of studying pathogen gene essentiality in polymicrobial communities.

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