Early Transcriptome Responses of the Bovine Midcycle Corpus Luteum to Prostaglandin F2α Includes Cytokine Signaling

Overview

Journal Mol Cell Endocrinol

Specialties Cell Biology
Endocrinology
Molecular Biology

Date 2017 May 28

PMID 28549990

Citations 14

Authors

Heather Talbott

Xiaoying Hou

Fang Qiu

Pan Zhang

Chittibabu Guda

Fang Yu

Robert A Cushman

Jennifer R Wood

Cheng Wang

Andrea S Cupp

John S Davis

Affiliations

Soon will be listed here.

Abstract

In ruminants, prostaglandin F2alpha (PGF2α)-mediated luteolysis is essential prior to estrous cycle resumption, and is a target for improving fertility. To deduce early PGF2α-provoked changes in the corpus luteum a short time-course (0.5-4 h) was performed on cows at midcycle. A microarray-determined transcriptome was established and examined by bioinformatic pathway analysis. Classic PGF2α effects were evident by changes in early response genes (FOS, JUN, ATF3) and prediction of active pathways (PKC, MAPK). Several cytokine transcripts were elevated and NF-κB and STAT activation were predicted by pathway analysis. Self-organizing map analysis grouped differentially expressed transcripts into ten mRNA expression patterns indicative of temporal signaling cascades. Comparison with two analogous datasets revealed a conserved group of 124 transcripts similarly altered by PGF2α treatment, which both, directly and indirectly, indicated cytokine activation. Elevated levels of cytokine transcripts after PGF2α and predicted activation of cytokine pathways implicate inflammatory reactions early in PGF2α-mediated luteolysis.

Citing Articles

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