Seasonal Dynamics of Extraradical Mycelium and Mycorrhizas in a Black Truffle (Tuber Melanosporum) Plantation

Overview

Journal Mycorrhiza

Date 2017 May 27

PMID 28547294

Citations 5

Authors

Mikel Queralt

Javier Parlade

Joan Pera

Ana Maria DE Miguel

Affiliations

Soon will be listed here.

Abstract

Seasonal dynamics of black truffle (Tuber melanosporum) extraradical mycelium as well as the associated mycorrhizal community have been evaluated in a 16-year-old plantation with productive and non-productive trees. Mycelium biomass was seasonally quantified by real-time PCR over two consecutive years and the correlation with environmental variables explored. Extraradical mycelium biomass varied seasonally and between the two consecutive years, being correlated with the precipitation that occurred 1 month before sampling. In addition, productive trees had more mycelium in the brûlé area than non-productive trees did. The ectomycorrhizal community composition inside the burnt areas was seasonally evaluated during a year. Ten mycorrhizal morphotypes were detected; T. melanosporum was the most abundant in productive and non-productive trees. Black truffle mycorrhizas were more abundant (mycorrhizal tips per unit of soil volume) in productive trees, and no seasonal variation was observed. The occurrence of black truffle mycorrhizas was significantly and positively correlated with the biomass of extraradical mycelium. The mycorrhizal community within the brûlé areas was significantly different between productive and non-productive trees, and no variation was detected between seasons. The assessment of the fungal vegetative structures in a mature plantation is of paramount importance to develop trufficulture methods based on the knowledge of the biological cycle of the fungus and its relationships with the associated ectomycorrhizal communities.

Citing Articles

Ectomycorrhizal Fungal Community and Ascoma Production in a Declining Plantation.

Ori F, Leonardi M, Puliga F, Lancellotti E, Pacioni G, Iotti M J Fungi (Basel). 2023; 9(6).

PMID: 37367614 PMC: 10301055. DOI: 10.3390/jof9060678.

Successes and challenges in the sustainable cultivation of edible mycorrhizal fungi - furthering the dream.

Guerin-Laguette A Mycoscience. 2023; 62(1):10-28.

PMID: 37090021 PMC: 9157773. DOI: 10.47371/mycosci.2020.11.007.

Design and Validation of qPCR-Specific Primers for Quantification of the Marketed and in Soil.

Arenas F, Morte A, Navarro-Rodenas A J Fungi (Basel). 2022; 8(10).

PMID: 36294660 PMC: 9605127. DOI: 10.3390/jof8101095.

White mulch and irrigation increase black truffle soil mycelium when competing with summer truffle in young truffle orchards.

Pinuela Y, Alday J, Oliach D, Castano C, Bolano F, Colinas C Mycorrhiza. 2021; 31(3):371-382.

PMID: 33515357 DOI: 10.1007/s00572-020-01018-x.

Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests.

Marjanovic Z, Nawaz A, Stevanovic K, Saljnikov E, Macek I, Oehl F Microorganisms. 2020; 8(9).

PMID: 32878332 PMC: 7563819. DOI: 10.3390/microorganisms8091331.

References

Taschen E, Sauve M, Taudiere A, Parlade J, Selosse M, Richard F . Whose truffle is this? Distribution patterns of ectomycorrhizal fungal diversity in Tuber melanosporum brûlés developed in multi-host Mediterranean plant communities. Environ Microbiol. 2014; 17(8):2747-61. DOI: 10.1111/1462-2920.12741. View

Hortal S, Pera J, Parlade J . Tracking mycorrhizas and extraradical mycelium of the edible fungus Lactarius deliciosus under field competition with Rhizopogon spp. Mycorrhiza. 2008; 18(2):69-77. DOI: 10.1007/s00572-007-0160-3. View

Rubini A, Belfiori B, Riccioni C, Tisserant E, Arcioni S, Martin F . Isolation and characterization of MAT genes in the symbiotic ascomycete Tuber melanosporum. New Phytol. 2010; 189(3):710-722. DOI: 10.1111/j.1469-8137.2010.03492.x. View

Napoli C, Mello A, Borra A, Vizzini A, Sourzat P, Bonfante P . Tuber melanosporum, when dominant, affects fungal dynamics in truffle grounds. New Phytol. 2009; 185(1):237-47. DOI: 10.1111/j.1469-8137.2009.03053.x. View

Sanchez S, Agreda T, Agueda B, Martin M, DE Miguel A, Barriuso J . Persistence and detection of black truffle ectomycorrhizas in plantations: comparison between two field detection methods. Mycorrhiza. 2014; 24 Suppl 1:S39-46. DOI: 10.1007/s00572-014-0560-0. View

De la Varga H, Agueda B, Martinez-Pena F, Parlade J, Pera J . Quantification of extraradical soil mycelium and ectomycorrhizas of Boletus edulis in a Scots pine forest with variable sporocarp productivity. Mycorrhiza. 2011; 22(1):59-68. DOI: 10.1007/s00572-011-0382-2. View

Murat C . Forty years of inoculating seedlings with truffle fungi: past and future perspectives. Mycorrhiza. 2014; 25(1):77-81. DOI: 10.1007/s00572-014-0593-4. View

Liu B, Fischer C, Bonet J, Olivera A, Inchusta A, Colinas C . Pattern of Tuber melanosporum extramatrical mycelium expansion over a 20-year chronosequence in Quercus ilex-truffle orchards. Mycorrhiza. 2014; 24 Suppl 1:S47-54. DOI: 10.1007/s00572-014-0559-6. View

Gryndler M, Trilcova J, Hrselova H, Streiblova E, Gryndlerova H, Jansa J . Tuber aestivum Vittad. mycelium quantified: advantages and limitations of a qPCR approach. Mycorrhiza. 2012; 23(5):341-8. DOI: 10.1007/s00572-012-0475-6. View

10.

Iotti M, Leonardi M, Lancellotti E, Salerni E, Oddis M, Leonardi P . Spatio-temporal dynamic of Tuber magnatum mycelium in natural truffle grounds. PLoS One. 2014; 9(12):e115921. PMC: 4275250. DOI: 10.1371/journal.pone.0115921. View

11.

Bonito G . Fast DNA-based identification of the black truffle Tuber melanosporum with direct PCR and species-specific primers. FEMS Microbiol Lett. 2009; 301(2):171-5. DOI: 10.1111/j.1574-6968.2009.01812.x. View

12.

Bustin S, Benes V, Garson J, Hellemans J, Huggett J, Kubista M . The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009; 55(4):611-22. DOI: 10.1373/clinchem.2008.112797. View

13.

De la Varga H, Agueda B, Agreda T, Martinez-Pena F, Parlade J, Pera J . Seasonal dynamics of Boletus edulis and Lactarius deliciosus extraradical mycelium in pine forests of central Spain. Mycorrhiza. 2013; 23(5):391-402. DOI: 10.1007/s00572-013-0481-3. View

14.

Parlade J, De la Varga H, De Miguel A, Saez R, Pera J . Quantification of extraradical mycelium of Tuber melanosporum in soils from truffle orchards in northern Spain. Mycorrhiza. 2012; 23(2):99-106. DOI: 10.1007/s00572-012-0454-y. View

15.

DE Miguel A, Agueda B, Saez R, Sanchez S, Parlade J . Diversity of ectomycorrhizal Thelephoraceae in Tuber melanosporum-cultivated orchards of Northern Spain. Mycorrhiza. 2015; 26(3):227-36. DOI: 10.1007/s00572-015-0665-0. View

16.

Landeweert R, Leeflang P, Kuyper T, Hoffland E, Rosling A, Wernars K . Molecular identification of ectomycorrhizal mycelium in soil horizons. Appl Environ Microbiol. 2003; 69(1):327-33. PMC: 152382. DOI: 10.1128/AEM.69.1.327-333.2003. View

17.

Raidl S, Bonfigli R, Agerer R . Calibration of quantitative real-time TaqMan PCR by correlation with hyphal biomass and ITS copies in mycelia of Piloderma croceum. Plant Biol (Stuttg). 2006; 7(6):713-7. DOI: 10.1055/s-2005-873003. View

18.

Kjoller R . Disproportionate abundance between ectomycorrhizal root tips and their associated mycelia. FEMS Microbiol Ecol. 2006; 58(2):214-24. DOI: 10.1111/j.1574-6941.2006.00166.x. View

19.

Sims S, Hendricks J, Mitchell R, Kuehn K, Pecot S . Nitrogen decreases and precipitation increases ectomycorrhizal extramatrical mycelia production in a longleaf pine forest. Mycorrhiza. 2007; 17(4):299-309. DOI: 10.1007/s00572-007-0105-x. View

20.

Zampieri E, Murat C, Cagnasso M, Bonfante P, Mello A . Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatum truffle-ground. FEMS Microbiol Ecol. 2009; 71(1):43-9. DOI: 10.1111/j.1574-6941.2009.00783.x. View