Antibiofilm Activity and Mode of Action of DMSO Alone and Its Combination with Afatinib Against Gram-negative Pathogens

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 2017 May 26

PMID 28540585

Citations 7

Authors

Mohd Fakharul Zaman Raja Yahya

Zazali Alias

Saiful Anuar Karsani

Affiliations

Soon will be listed here.

Abstract

Biofilms are complex microbial communities that tend to attach to either biotic or abiotic surface. Enclosed in a self-produced extracellular polymeric substance (EPS) matrix, the biofilms often cause persistent infections. The objective of this study was to investigate the antibiofilm activity of dimethyl sulfoxide (DMSO) and afatinib against Gram-negative pathogens. Test microorganisms used in this study were Escherichia coli ATCC 1299, Pseudomonas aeruginosa ATCC 10145, and Salmonella typhimurium ATCC 14028. Biofilms were developed in 96-well microplate at 37°C for 24 h. Following removal of non-adherent cells, analysis of biofilm viability, biofilm biomass, and extracellular polymeric substances (EPS) matrix were performed using resazurin assay, crystal violet assay, and attenuated total reflectance fourier transform infrared (ATR-FTIR) spectroscopy, respectively. Bradford protein assay was conducted to determine the total amount of EPS proteins. The results demonstrated that both 32% DMSO alone and its combination with 3.2 μg/mL afatinib were effective in killing biofilm cells and reducing biofilm biomass. IR spectral variations of EPS matrix of biofilms in the range between 1700 and 900 cm were also observed. Reduction in EPS proteins verified the chemical modifications of EPS matrix. In conclusion, 32% DMSO alone and its combination with 3.2 μg/mL afatinib showed remarkable antibiofilm activities against Gram-negative pathogens. It was suggested that the biofilm inhibition was mediated by the chemical modification of EPS matrix.

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References

Ulanowska K, Tkaczyk A, Konopa G, Wegrzyn G . Differential antibacterial activity of genistein arising from global inhibition of DNA, RNA and protein synthesis in some bacterial strains. Arch Microbiol. 2005; 184(5):271-8. DOI: 10.1007/s00203-005-0063-7. View

Skogman M, Vuorela P, Fallarero A . Combining biofilm matrix measurements with biomass and viability assays in susceptibility assessments of antimicrobials against Staphylococcus aureus biofilms. J Antibiot (Tokyo). 2012; 65(9):453-9. DOI: 10.1038/ja.2012.49. View

Costerton J, Stewart P, Greenberg E . Bacterial biofilms: a common cause of persistent infections. Science. 1999; 284(5418):1318-22. DOI: 10.1126/science.284.5418.1318. View

Harrison J, Turner R, Joo D, Stan M, Chan C, Allan N . Copper and quaternary ammonium cations exert synergistic bactericidal and antibiofilm activity against Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2008; 52(8):2870-81. PMC: 2493123. DOI: 10.1128/AAC.00203-08. View

Entcheva-Dimitrov P, Spormann A . Dynamics and control of biofilms of the oligotrophic bacterium Caulobacter crescentus. J Bacteriol. 2004; 186(24):8254-66. PMC: 532430. DOI: 10.1128/JB.186.24.8254-8266.2004. View

Sauer K . The genomics and proteomics of biofilm formation. Genome Biol. 2003; 4(6):219. PMC: 193612. DOI: 10.1186/gb-2003-4-6-219. View

Basch H, GADEBUSCH H . In vitro antimicrobial activity of dimethylsulfoxide. Appl Microbiol. 1968; 16(12):1953-4. PMC: 547808. DOI: 10.1128/am.16.12.1953-1954.1968. View

Suci P, Mittelman M, Yu F, Geesey G . Investigation of ciprofloxacin penetration into Pseudomonas aeruginosa biofilms. Antimicrob Agents Chemother. 1994; 38(9):2125-33. PMC: 284696. DOI: 10.1128/AAC.38.9.2125. View

Branda S, Vik S, Friedman L, Kolter R . Biofilms: the matrix revisited. Trends Microbiol. 2005; 13(1):20-6. DOI: 10.1016/j.tim.2004.11.006. View

10.

Bartsch W, Sponer G, DIETMANN K, Fuchs G . Acute toxicity of various solvents in the mouse and rat. LD50 of ethanol, diethylacetamide, dimethylformamide, dimethylsulfoxide, glycerine, N-methylpyrrolidone, polyethylene glycol 400, 1,2-propanediol and Tween 20. Arzneimittelforschung. 1976; 26(8):1581-3. View

11.

Halstead F, Rauf M, Moiemen N, Bamford A, Wearn C, Fraise A . The Antibacterial Activity of Acetic Acid against Biofilm-Producing Pathogens of Relevance to Burns Patients. PLoS One. 2015; 10(9):e0136190. PMC: 4566994. DOI: 10.1371/journal.pone.0136190. View

12.

Stewart P, Franklin M . Physiological heterogeneity in biofilms. Nat Rev Microbiol. 2008; 6(3):199-210. DOI: 10.1038/nrmicro1838. View

13.

Korstgens V, Flemming H, Wingender J, Borchard W . Uniaxial compression measurement device for investigation of the mechanical stability of biofilms. J Microbiol Methods. 2001; 46(1):9-17. DOI: 10.1016/s0167-7012(01)00248-2. View

14.

Spoering A, Lewis K . Biofilms and planktonic cells of Pseudomonas aeruginosa have similar resistance to killing by antimicrobials. J Bacteriol. 2001; 183(23):6746-51. PMC: 95513. DOI: 10.1128/JB.183.23.6746-6751.2001. View

15.

Karatan E, Watnick P . Signals, regulatory networks, and materials that build and break bacterial biofilms. Microbiol Mol Biol Rev. 2009; 73(2):310-47. PMC: 2698413. DOI: 10.1128/MMBR.00041-08. View

16.

Sun W, Tanaka T, Magle C, Huang W, Southall N, Huang R . Chemical signatures and new drug targets for gametocytocidal drug development. Sci Rep. 2014; 4:3743. PMC: 3894558. DOI: 10.1038/srep03743. View

17.

Zuurmond W, Langendijk P, Bezemer P, Brink H, de Lange J, van Loenen A . Treatment of acute reflex sympathetic dystrophy with DMSO 50% in a fatty cream. Acta Anaesthesiol Scand. 1996; 40(3):364-7. DOI: 10.1111/j.1399-6576.1996.tb04446.x. View

18.

Zhang X, Munster P . New protein kinase inhibitors in breast cancer: afatinib and neratinib. Expert Opin Pharmacother. 2014; 15(9):1277-88. DOI: 10.1517/14656566.2014.913570. View

19.

Elisia I, Nakamura H, Lam V, Hofs E, Cederberg R, Cait J . DMSO Represses Inflammatory Cytokine Production from Human Blood Cells and Reduces Autoimmune Arthritis. PLoS One. 2016; 11(3):e0152538. PMC: 4816398. DOI: 10.1371/journal.pone.0152538. View

20.

Wang H, Ding S, Wang G, Xu X, Zhou G . In situ characterization and analysis of Salmonella biofilm formation under meat processing environments using a combined microscopic and spectroscopic approach. Int J Food Microbiol. 2013; 167(3):293-302. DOI: 10.1016/j.ijfoodmicro.2013.10.005. View