[Expression of FABP7 in Mouse Placenta Tissue and Human Trophoblast HTR-8/Svneo Cells]

Overview

Journal Nan Fang Yi Ke Da Xue Xue Bao

Specialty General Medicine

Date 2017 May 26

PMID 28539280

Authors

Liu Tian

Hui-Qi Liao

Hui Yang

Ni Ma

Chang-Jun Zhang

Hong-Lu Diao

Affiliations

Soon will be listed here.

Abstract

Objective: To detect the expression of FABP7 in the placenta of pregnant mice and in HTR-8/Svneo cells.

Methods: Real-time PCR and immunofluorescence were used to detect FABP7 mRNA and protein expressions in the uterine and placental tissue of pregnant mice at different days of gestation. FABP7 expression was also detected in cultured HTR-8/Svneo cells using immunofluorescence assay. The mice were treated with E, P or their combination for 6 and 24 h and Fabp7 mRNA level in the uterus was detected with real-time PCR.

Results: At 7.5-10.5 days of gestation, the pregnant mice showed positive expressions of Fabp7 mRNA in the uterus and placenta, and FABP7 protein was detected in the decidualized cells and trophoblast giant cells. The expressions of FABP7 were detected at both the mRNA and protein levels in cultured HTR-8/Svneo cells. In mice treated with P4 alone or with E+P for 6 and 24 h, the expression level of Fabp7 mRNA was upregulated in the uterus. Fabp7 upregulation was observed in mice at 24 h following E treatment but not at 6 h.

Conclusion: FABP7 is expressed in trophoblast giant cells and decidual cells in the placental tissue of mice and in cultured HTR-8/Svneo cells, suggesting the involvement of FABP7 in placental development and in maintenance of pregnancy. E and P can regulate the expression of FABP7 in mouse uterus.

References

Varvarigou A . Intrauterine growth restriction as a potential risk factor for disease onset in adulthood. J Pediatr Endocrinol Metab. 2010; 23(3):215-24. DOI: 10.1515/jpem.2010.23.3.215. View

Gauster M, Hiden U, Blaschitz A, Frank S, Lang U, Alvino G . Dysregulation of placental endothelial lipase and lipoprotein lipase in intrauterine growth-restricted pregnancies. J Clin Endocrinol Metab. 2007; 92(6):2256-63. DOI: 10.1210/jc.2006-2403. View

Elsherbiny M, Goruk S, Monckton E, Richard C, Brun M, Emara M . Long-Term Effect of Docosahexaenoic Acid Feeding on Lipid Composition and Brain Fatty Acid-Binding Protein Expression in Rats. Nutrients. 2015; 7(10):8802-17. PMC: 4632453. DOI: 10.3390/nu7105433. View

Wang Y, Li X, Liu J, Fu W . Antenatal taurine supplementation in fetal rats with growth restriction improves neural stem cell proliferation by inhibiting the activities of Rho family factors. J Matern Fetal Neonatal Med. 2017; 31(11):1454-1461. DOI: 10.1080/14767058.2017.1319353. View

Maximin E, Langelier B, Aioun J, Al-Gubory K, Bordat C, Lavialle M . Fatty acid binding protein 7 and n-3 poly unsaturated fatty acid supply in early rat brain development. Dev Neurobiol. 2015; 76(3):287-97. DOI: 10.1002/dneu.22314. View

Tantbirojn P, Crum C, Parast M . Pathophysiology of placenta creta: the role of decidua and extravillous trophoblast. Placenta. 2008; 29(7):639-45. DOI: 10.1016/j.placenta.2008.04.008. View

Haggarty P . Meeting the fetal requirement for polyunsaturated fatty acids in pregnancy. Curr Opin Clin Nutr Metab Care. 2014; 17(2):151-5. DOI: 10.1097/MCO.0000000000000036. View

Liu R, Graham K, Glubrecht D, Lai R, Mackey J, Godbout R . A fatty acid-binding protein 7/RXRβ pathway enhances survival and proliferation in triple-negative breast cancer. J Pathol. 2012; 228(3):310-21. DOI: 10.1002/path.4001. View

Mishima T, Miner J, Morizane M, Stahl A, Sadovsky Y . The expression and function of fatty acid transport protein-2 and -4 in the murine placenta. PLoS One. 2011; 6(10):e25865. PMC: 3197585. DOI: 10.1371/journal.pone.0025865. View

10.

Jansson T, Myatt L, Powell T . The role of trophoblast nutrient and ion transporters in the development of pregnancy complications and adult disease. Curr Vasc Pharmacol. 2009; 7(4):521-33. DOI: 10.2174/157016109789043982. View

11.

Burrows T, King A, Loke Y . Trophoblast migration during human placental implantation. Hum Reprod Update. 1996; 2(4):307-21. DOI: 10.1093/humupd/2.4.307. View

12.

Islam A, Kagawa Y, Sharifi K, Ebrahimi M, Miyazaki H, Yasumoto Y . Fatty Acid Binding Protein 3 Is Involved in n-3 and n-6 PUFA transport in mouse trophoblasts. J Nutr. 2014; 144(10):1509-16. DOI: 10.3945/jn.114.197202. View

13.

Sibley C . Understanding placental nutrient transfer--why bother? New biomarkers of fetal growth. J Physiol. 2009; 587(Pt 14):3431-40. PMC: 2742272. DOI: 10.1113/jphysiol.2009.172403. View

14.

Li X, Li F, Liu J, Wang Y, Fu W . [Effect of antepartum taurine supplementation in regulating the activity of Rho family factors and promoting the proliferation of neural stem cells in neonatal rats with fetal growth restriction]. Zhongguo Dang Dai Er Ke Za Zhi. 2016; 18(11):1158-1165. PMC: 7389851. View

15.

Morihiro Y, Yasumoto Y, Vaidyan L, Sadahiro H, Uchida T, Inamura A . Fatty acid binding protein 7 as a marker of glioma stem cells. Pathol Int. 2013; 63(11):546-53. DOI: 10.1111/pin.12109. View

16.

Besnard P, Foucaud L, Mallordy A, Berges C, Kaikaus R, Bernard A . Expression of fatty acid binding protein in the liver during pregnancy and lactation in the rat. Biochim Biophys Acta. 1995; 1258(2):153-8. DOI: 10.1016/0005-2760(95)00114-r. View

17.

Barker D, Bagby S, Hanson M . Mechanisms of disease: in utero programming in the pathogenesis of hypertension. Nat Clin Pract Nephrol. 2006; 2(12):700-7. DOI: 10.1038/ncpneph0344. View

18.

Larque E, Ruiz-Palacios M, Koletzko B . Placental regulation of fetal nutrient supply. Curr Opin Clin Nutr Metab Care. 2013; 16(3):292-7. DOI: 10.1097/MCO.0b013e32835e3674. View

19.

Campbell F, Bush P, Veerkamp J, Dutta-Roy A . Detection and cellular localization of plasma membrane-associated and cytoplasmic fatty acid-binding proteins in human placenta. Placenta. 1998; 19(5-6):409-15. DOI: 10.1016/s0143-4004(98)90081-9. View

20.

Barker D . Adult consequences of fetal growth restriction. Clin Obstet Gynecol. 2006; 49(2):270-83. DOI: 10.1097/00003081-200606000-00009. View