Integrative Suppression of DnaA46 by Broad Host-range Plasmid R1162

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1988 Dec 1

PMID 2853828

Citations 1

Authors

M A Brasch

R J Meyer

Affiliations

Soon will be listed here.

Abstract

Replication of plasmid R1162 DNA does not require the product of the dnaA gene. An integrated copy of the plasmid can suppress the temperature-sensitive dnaA46 allele when (1) additional plasmid copies are present in the cytoplasm and (2) an inactive replication origin, generated by deletion, is also present in the chromosome. We propose that the inactive origin sets the rate of initiation of chromosome replication at a level compatible with cell viability, possibly by providing additional binding sites for an R1162-encoded protein that is rate-limiting for plasmid replication.

Citing Articles

Replication and conjugative mobilization of broad host-range IncQ plasmids.

Meyer R Plasmid. 2009; 62(2):57-70.

PMID: 19465049 PMC: 2752045. DOI: 10.1016/j.plasmid.2009.05.001.

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