Ca2+-dependent Phosphoregulation of the Plasma Membrane Ca2+-ATPase ACA8 Modulates Stimulus-induced Calcium Signatures

Overview

Journal J Exp Bot

Publisher Oxford University Press

Specialty Biology

Date 2017 May 23

PMID 28531251

Citations 30

Authors

Alex Costa

Laura Luoni

Claudia Adriana Marrano

Kenji Hashimoto

Philipp Koster

Sonia Giacometti

Maria Ida De Michelis

Jorg Kudla

Maria Cristina Bonza

Affiliations

Soon will be listed here.

Abstract

Ca2+ signals are transient, hence, upon a stimulus-induced increase in cytosolic Ca2+ concentration, cells have to re-establish resting Ca2+ levels. Ca2+ extrusion is operated by a wealth of transporters, such as Ca2+ pumps and Ca2+/H+ antiporters, which often require a rise in Ca2+ concentration to be activated. Here, we report a regulatory fine-tuning mechanism of the Arabidopsis thaliana plasma membrane-localized Ca2+-ATPase isoform ACA8 that is mediated by calcineurin B-like protein (CBL) and CBL-interacting protein kinase (CIPK) complexes. We show that two CIPKs (CIPK9 and CIPK14) are able to interact with ACA8 in vivo and phosphorylate it in vitro. Transient co-overexpression of ACA8 with CIPK9 and the plasma membrane Ca2+ sensor CBL1 in tobacco leaf cells influences nuclear Ca2+ dynamics, specifically reducing the height of the second peak of the wound-induced Ca2+ transient. Stimulus-induced Ca2+ transients in mature leaves and seedlings of an aca8 T-DNA insertion line exhibit altered dynamics when compared with the wild type. Altogether our results identify ACA8 as a prominent in vivo regulator of cellular Ca2+ dynamics and reveal the existence of a Ca2+-dependent CBL-CIPK-mediated regulatory feedback mechanism, which crucially functions in the termination of Ca2+ signals.

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