Advances in the Isolation of Specific Monoclonal Rabbit Antibodies

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2017 May 23

PMID 28529510

Citations 15

Authors

Zaibao Zhang

Huijuan Liu

Qian Guan

Lei Wang

Hongyu Yuan

Affiliations

Soon will be listed here.

Abstract

The rabbit monoclonal antibodies (mAbs) have advantages in pharmaceuticals and diagnostics with high affinity and specificity. During the past decade, many techniques have been developed for isolating rabbit mAbs, including single B cell antibody technologies. This review describes the basic characterization of rabbit antibody repertoire and summarizes methods of hybridoma technologies, phage display platform, and single B cell antibody technologies. With advances in antibody function and repertoire analysis, rabbit mAbs will be widely used in therapeutic applications in the coming years.

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References

Glukhova X, Prusakova O, Trizna J, Zaripov M, Afanaseva G, Glukhov A . Updates on the Production of Therapeutic Antibodies Using Human Hybridoma Technique. Curr Pharm Des. 2015; 22(7):870-8. DOI: 10.2174/1381612822666151223102845. View

Murai T, Ueda M, Yamamura M, Atomi H, Shibasaki Y, Kamasawa N . Construction of a starch-utilizing yeast by cell surface engineering. Appl Environ Microbiol. 1997; 63(4):1362-6. PMC: 168429. DOI: 10.1128/aem.63.4.1362-1366.1997. View

Tiller T, Schuster I, Deppe D, Siegers K, Strohner R, Herrmann T . A fully synthetic human Fab antibody library based on fixed VH/VL framework pairings with favorable biophysical properties. MAbs. 2013; 5(3):445-70. PMC: 4169037. DOI: 10.4161/mabs.24218. View

Clargo A, Hudson A, Ndlovu W, Wootton R, Cremin L, ODowd V . The rapid generation of recombinant functional monoclonal antibodies from individual, antigen-specific bone marrow-derived plasma cells isolated using a novel fluorescence-based method. MAbs. 2014; 6(1):143-59. PMC: 3929438. DOI: 10.4161/mabs.27044. View

Olsson L, Kaplan H . Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity. Proc Natl Acad Sci U S A. 1980; 77(9):5429-31. PMC: 350072. DOI: 10.1073/pnas.77.9.5429. View

Marks J, Hoogenboom H, Bonnert T, McCafferty J, Griffiths A, Winter G . By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J Mol Biol. 1991; 222(3):581-97. DOI: 10.1016/0022-2836(91)90498-u. View

Rothe C, Urlinger S, Lohning C, Prassler J, Stark Y, Jager U . The human combinatorial antibody library HuCAL GOLD combines diversification of all six CDRs according to the natural immune system with a novel display method for efficient selection of high-affinity antibodies. J Mol Biol. 2008; 376(4):1182-200. DOI: 10.1016/j.jmb.2007.12.018. View

Benhar I . Design of synthetic antibody libraries. Expert Opin Biol Ther. 2007; 7(5):763-79. DOI: 10.1517/14712598.7.5.763. View

Shawler D, Bartholomew R, Smith L, Dillman R . Human immune response to multiple injections of murine monoclonal IgG. J Immunol. 1985; 135(2):1530-5. View

10.

Flego M, Ascione A, Cianfriglia M, Vella S . Clinical development of monoclonal antibody-based drugs in HIV and HCV diseases. BMC Med. 2013; 11:4. PMC: 3565905. DOI: 10.1186/1741-7015-11-4. View

11.

Shim H . Synthetic approach to the generation of antibody diversity. BMB Rep. 2015; 48(9):489-94. PMC: 4641231. DOI: 10.5483/bmbrep.2015.48.9.120. View

12.

Rakestraw J, Aird D, Aha P, Baynes B, Lipovsek D . Secretion-and-capture cell-surface display for selection of target-binding proteins. Protein Eng Des Sel. 2011; 24(6):525-30. DOI: 10.1093/protein/gzr008. View

13.

Winter G, Griffiths A, Hawkins R, Hoogenboom H . Making antibodies by phage display technology. Annu Rev Immunol. 1994; 12:433-55. DOI: 10.1146/annurev.iy.12.040194.002245. View

14.

Lowe D, Jermutus L . Combinatorial protein biochemistry for therapeutics and proteomics. Curr Pharm Biotechnol. 2004; 5(1):17-27. DOI: 10.2174/1389201043489585. View

15.

Feng L, Wang X, Jin H . Rabbit monoclonal antibody: potential application in cancer therapy. Am J Transl Res. 2011; 3(3):269-74. PMC: 3102571. View

16.

Steinberger P, Sutton J, Rader C, Elia M, Barbas 3rd C . Generation and characterization of a recombinant human CCR5-specific antibody. A phage display approach for rabbit antibody humanization. J Biol Chem. 2000; 275(46):36073-8. DOI: 10.1074/jbc.M002765200. View

17.

Ojima-Kato T, Hashimura D, Kojima T, Minabe S, Nakano H . In vitro generation of rabbit anti-Listeria monocytogenes monoclonal antibody using single cell based RT-PCR linked cell-free expression systems. J Immunol Methods. 2015; 427:58-65. DOI: 10.1016/j.jim.2015.10.001. View

18.

Sethupathi P, Yam P, Knight K . Rabbit monoclonal antibodies: generating a fusion partner to produce rabbit-rabbit hybridomas. Proc Natl Acad Sci U S A. 1995; 92(20):9348-52. PMC: 40982. DOI: 10.1073/pnas.92.20.9348. View

19.

Roberts R, Szostak J . RNA-peptide fusions for the in vitro selection of peptides and proteins. Proc Natl Acad Sci U S A. 1997; 94(23):12297-302. PMC: 24913. DOI: 10.1073/pnas.94.23.12297. View

20.

Raybould T, Takahashi M . Production of stable rabbit-mouse hybridomas that secrete rabbit mAb of defined specificity. Science. 1988; 240(4860):1788-90. DOI: 10.1126/science.3289119. View