Lifetime-resolved Photoacoustic (LPA) Spectroscopy for Monitoring Oxygen Change and Photodynamic Therapy (PDT)

Overview

Journal Proc SPIE Int Soc Opt Eng

Date 2017 May 23

PMID 28529402

Citations 1

Authors

Janggun Jo

Chang Heon Lee

Raoul Kopelman

Xueding Wang

Affiliations

Soon will be listed here.

Abstract

The Methylene Blue loaded Polyacrylamide Nanoparticles (MB-PAA NPs) are used for oxygen sensing and Photodynamic therapy (PDT), a promising therapeutic modality employed for various tumors, with distinct advantages of delivery of biomedical agents and protection from other bio-molecules overcoming inherent limitations of molecular dyes. Lifetime-resolved photoacoustic spectroscopy using quenched-phosphorescence method is applied with MB-PAA NPs so as to sense oxygen, while the same light source is used for PDT. The dye is excited by absorbing 650 nm wavelength light from a pump laser to reach triplet state. The probe laser at 810 nm wavelength is used to excite the first triplet state at certain delayed time to measure the dye lifetime which indicates oxygen concentration. The 9L cells (106 cells/ml) incubated with MB-PAA NP solution are used for monitoring oxygen level change during PDT in situ test. The oxygen level and PDT efficacy are confirmed with a commercial oximeter, and fluorescence microscope imaging and flow cytometry results. This technique with the MB-PAA NPs allowed us to demonstrate a potential non-invasive theragnostic operation, by monitoring oxygen depletion during PDT in situ, without the addition of secondary probes. Here, we demonstrate this theragnostic operation, in vitro, performing PDT while monitoring oxygen depletion. We also show the correlation between O2 depletion and cell death.

Citing Articles

Theranostic Liposomes with Hypoxia-Activated Prodrug to Effectively Destruct Hypoxic Tumors Post-Photodynamic Therapy.

Feng L, Cheng L, Dong Z, Tao D, Barnhart T, Cai W ACS Nano. 2016; 11(1):927-937.

PMID: 28027442 PMC: 5372701. DOI: 10.1021/acsnano.6b07525.

References

Henderson B, Busch T, Vaughan L, Frawley N, Babich D, Sosa T . Photofrin photodynamic therapy can significantly deplete or preserve oxygenation in human basal cell carcinomas during treatment, depending on fluence rate. Cancer Res. 2000; 60(3):525-9. View

Busch T, Hahn S, Evans S, Koch C . Depletion of tumor oxygenation during photodynamic therapy: detection by the hypoxia marker EF3 [2-(2-nitroimidazol-1[H]-yl)-N-(3,3,3-trifluoropropyl)acetamide ]. Cancer Res. 2000; 60(10):2636-42. View

Hopper C . Photodynamic therapy: a clinical reality in the treatment of cancer. Lancet Oncol. 2002; 1:212-9. DOI: 10.1016/s1470-2045(00)00166-2. View

Brown S, Brown E, Walker I . The present and future role of photodynamic therapy in cancer treatment. Lancet Oncol. 2004; 5(8):497-508. DOI: 10.1016/S1470-2045(04)01529-3. View

Tang W, Xu H, Kopelman R, Philbert M . Photodynamic characterization and in vitro application of methylene blue-containing nanoparticle platforms. Photochem Photobiol. 2004; 81(2):242-9. DOI: 10.1562/2004-05-24-RA-176.1. View

Huang Z . A review of progress in clinical photodynamic therapy. Technol Cancer Res Treat. 2005; 4(3):283-93. PMC: 1317568. DOI: 10.1177/153303460500400308. View

Tang W, Xu H, Park E, Philbert M, Kopelman R . Encapsulation of methylene blue in polyacrylamide nanoparticle platforms protects its photodynamic effectiveness. Biochem Biophys Res Commun. 2008; 369(2):579-83. PMC: 2366160. DOI: 10.1016/j.bbrc.2008.02.066. View

Ashkenazi S, Huang S, Horvath T, Koo Y, Kopelman R, Kopelman R . Photoacoustic probing of fluorophore excited state lifetime with application to oxygen sensing. J Biomed Opt. 2008; 13(3):034023. DOI: 10.1117/1.2927466. View

Chatterjee D, Fong L, Zhang Y . Nanoparticles in photodynamic therapy: an emerging paradigm. Adv Drug Deliv Rev. 2008; 60(15):1627-37. DOI: 10.1016/j.addr.2008.08.003. View

10.

Ashkenazi S . Photoacoustic lifetime imaging of dissolved oxygen using methylene blue. J Biomed Opt. 2010; 15(4):040501. DOI: 10.1117/1.3465548. View

11.

Lee Y, Kopelman R . Polymeric nanoparticles for photodynamic therapy. Methods Mol Biol. 2011; 726:151-78. DOI: 10.1007/978-1-61779-052-2_11. View

12.

Qin M, Hah H, Kim G, Nie G, Lee Y, Kopelman R . Methylene blue covalently loaded polyacrylamide nanoparticles for enhanced tumor-targeted photodynamic therapy. Photochem Photobiol Sci. 2011; 10(5):832-41. PMC: 3584693. DOI: 10.1039/c1pp05022b. View

13.

Nie G, Hah H, Kim G, Lee Y, Qin M, Ratani T . Hydrogel nanoparticles with covalently linked coomassie blue for brain tumor delineation visible to the surgeon. Small. 2012; 8(6):884-91. DOI: 10.1002/smll.201101607. View

14.

Avula U, Kim G, Lee Y, Morady F, Kopelman R, Kalifa J . Cell-specific nanoplatform-enabled photodynamic therapy for cardiac cells. Heart Rhythm. 2012; 9(9):1504-9. PMC: 3584703. DOI: 10.1016/j.hrthm.2012.05.011. View

15.

Ray A, Rajian J, Lee Y, Wang X, Kopelman R . Lifetime-based photoacoustic oxygen sensing in vivo. J Biomed Opt. 2012; 17(5):057004. PMC: 3381016. DOI: 10.1117/1.JBO.17.5.057004. View

16.

Ray A, Yoon H, Koo Lee Y, Kopelman R, Wang X . Sonophoric nanoprobe aided pH measurement in vivo using photoacoustic spectroscopy. Analyst. 2013; 138(11):3126-30. PMC: 4111223. DOI: 10.1039/c3an00093a. View

17.

Karamchand L, Kim G, Wang S, Hah H, Ray A, Jiddou R . Modulation of hydrogel nanoparticle intracellular trafficking by multivalent surface engineering with tumor targeting peptide. Nanoscale. 2013; 5(21):10327-44. PMC: 3823366. DOI: 10.1039/c3nr00908d. View

18.

Yoon H, Lou X, Chen Y, Lee Y, Yoon E, Kopelman R . Nano-photosensitizers Engineered to Generate a Tunable Mix of Reactive Oxygen Species, for Optimizing Photodynamic Therapy, Using a Microfluidic Device. Chem Mater. 2014; 26(4):1592-1600. PMC: 3970790. DOI: 10.1021/cm403505s. View

19.

Tardivo J, Del Giglio A, de Oliveira C, Gabrielli D, Junqueira H, Tada D . Methylene blue in photodynamic therapy: From basic mechanisms to clinical applications. Photodiagnosis Photodyn Ther. 2014; 2(3):175-91. DOI: 10.1016/S1572-1000(05)00097-9. View

20.

Shao Q, Ashkenazi S . Photoacoustic lifetime imaging for direct in vivo tissue oxygen monitoring. J Biomed Opt. 2015; 20(3):036004. PMC: 4351645. DOI: 10.1117/1.JBO.20.3.036004. View