Evaluation of Estrogen Receptor Dimers in Breast Carcinoma Cells: Visualization of Protein-Protein Interactions

Overview

Journal Acta Histochem Cytochem

Specialty Biochemistry

Date 2017 May 20

PMID 28522883

Citations 10

Authors

Erina Iwabuchi

Yasuhiro Miki

Katsuhiko Ono

Yoshiaki Onodera

Hironobu Sasano

Affiliations

Soon will be listed here.

Abstract

The estrogen receptor (ER) functions as a dimer and is involved in several different biological functions. However ER dimeric proteins have not been identified by methodologies. Structured illumination microscopy (SIM) has been recently developed, which enabled the localization of protein and protein interaction. Therefore, in this study, we firstly demonstrated that ERs formed both homodimers and heterodimers in breast carcinoma cell lines using Nikon's SIM (N-SIM). ERα/α homodimers were detected in the nuclei of both ERα-positive MCF-7 and T-47D cells; 23.0% and 13.4% of ERα proteins formed ERα/α homodimers, respectively. ERα/β heterodimers were also detected in MCF-7 and T-47D. Approximately 6.6% of both ERα and ERβ1 proteins formed ERα/β1 heterodimers in MCF-7. In addition, 18.1% and 22.4% of ERα and ERβ proteins formed ERα/β2 heterodimers and ERα/β5 heterodimers in MCF-7, respectively. In addition, by using proximity ligation assay (PLA) in MCF-7, estradiol-induced ERα/α homodimers and ERα/β1 heterodimers were both detected after 15 to 45 min of treatment and at 15 min, respectively. The percentage of total ER proteins could also be determined using N-SIM. By using both methods, it has become possible to evaluate precise localization and ratio of ER dimers among different cell types.

Citing Articles

Fluorescence-based techniques for investigating estrogen receptor dynamics.

Han K, Choi G, Kim T BMB Rep. 2024; 57(11):472-483.

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Visualization of the protein-protein interactions of hormone receptors in hormone-dependent cancer research.

Iwabuchi E, Miki Y, Suzuki T, Sasano H Endocr Oncol. 2023; 2(1):R132-R142.

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In Situ Proximity Ligation Assay to Visualize Protein-Protein Interactions in Tumor Specimens.

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Role of Cancer Stem-like Cells in the Process of Invasion and Mesenchymal Transformation by a Reconstituted Triple-negative Breast Cancer Cell Population Resistant to p53-induced Apoptosis.

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PMID: 36405550 PMC: 9631983. DOI: 10.1267/ahc.22-00076.

The Visualization of Protein-Protein Interactions in Breast Cancer: Deployment Study in Pathological Examination.

Iwabuchi E, Miki Y, Sasano H Acta Histochem Cytochem. 2022; 54(6):177-183.

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