Ultrasound and Microbubble Induced Release from Intracellular Compartments

Overview

Journal BMC Biotechnol

Publisher Biomed Central

Specialty Biotechnology

Date 2017 May 20

PMID 28521780

Citations 5

Authors

Farah Hussein

Costin Antonescu

Raffi Karshafian

Affiliations

Soon will be listed here.

Abstract

Background: Ultrasound and microbubbles (USMB) have been shown to enhance the intracellular uptake of molecules, generally thought to occur as a result of sonoporation. The underlying mechanism associated with USMB-enhanced intracellular uptake such as membrane disruption and endocytosis may also be associated with USMB-induced release of cellular materials to the extracellular milieu. This study investigates USMB effects on the molecular release from cells through membrane-disruption and exocytosis.

Results: USMB induced the release of 19% and 67% of GFP from the cytoplasm in viable and non-viable cells, respectively. Tfn release from early/recycling endosomes increased by 23% in viable cells upon USMB treatment. In addition, the MFI of LAMP-1 antibody increased by 50% in viable cells, suggesting USMB-stimulated lysosome exocytosis. In non-viable cells, labeling of LAMP-1 intracellular structures in the absence of cell permeabilization by detergents suggests that USMB-induced cell death correlates with lysosomal permeabilization.

Conclusions: In conclusion, USMB enhanced the molecular release from the cytoplasm, lysosomes, and early/recycling endosomes.

Citing Articles

Barrier-breaking effects of ultrasonic cavitation for drug delivery and biomarker release.

Hu Y, Wei J, Shen Y, Chen S, Chen X Ultrason Sonochem. 2023; 94:106346.

PMID: 36870921 PMC: 10040969. DOI: 10.1016/j.ultsonch.2023.106346.

AMPK is required for recovery from metabolic stress induced by ultrasound microbubble treatment.

Lo L, Uchenunu O, Botelho R, Antonescu C, Karshafian R iScience. 2023; 26(2):105883.

PMID: 36685038 PMC: 9845798. DOI: 10.1016/j.isci.2022.105883.

Synergistic enhancement of cell death by triple combination therapy of docetaxel, ultrasound and microbubbles, and radiotherapy on PC3 a prostate cancer cell line.

Almasri F, Karshafian R Heliyon. 2022; 8(8):e10213.

PMID: 36033334 PMC: 9404355. DOI: 10.1016/j.heliyon.2022.e10213.

Treatment of glaucomatous optic nerve damage using ginsenoside Rg1 mediated by ultrasound targeted microbubble destruction.

Wang L, Cao T, Chen H Exp Ther Med. 2018; 15(1):300-304.

PMID: 29375689 PMC: 5763670. DOI: 10.3892/etm.2017.5386.

Cationic gas-filled microbubbles for ultrasound-based nucleic acids delivery.

Delalande A, Bastie C, Pigeon L, Manta S, Lebertre M, Mignet N Biosci Rep. 2017; 37(6).

PMID: 29180378 PMC: 5741830. DOI: 10.1042/BSR20160619.

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