CaMKII Autophosphorylation Is Necessary for Optimal Integration of Ca Signals During LTP Induction, but Not Maintenance

Overview

Journal Neuron

Publisher Cell Press

Specialty Neurology

Date 2017 May 19

PMID 28521133

Citations 76

Authors

Jui-Yun Chang

Paula Parra-Bueno

Tal Laviv

Erzsebet M Szatmari

Seok-Jin R Lee

Ryohei Yasuda

Affiliations

Soon will be listed here.

Abstract

CaMKII plays a critical role in decoding calcium (Ca) signals to initiate long-lasting synaptic plasticity. However, the properties of CaMKII that mediate Ca signals in spines remain elusive. Here, we measured CaMKII activity in spines using fast-framing two-photon fluorescence lifetime imaging. Following each pulse during repetitive Ca elevations, CaMKII activity increased in a stepwise manner. Thr286 phosphorylation slows the decay of CaMKII and thus lowers the frequency required to induce spine plasticity by several fold. In the absence of Thr286 phosphorylation, increasing the stimulation frequency results in high peak mutant CaMKII activity that is sufficient for inducing plasticity. Our findings demonstrate that Thr286 phosphorylation plays an important role in induction of LTP by integrating Ca signals, and it greatly promotes, but is dispensable for, the activation of CaMKII and LTP.

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