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Pyrenoid Loss Impairs Carbon-concentrating Mechanism Induction and Alters Primary Metabolism in Chlamydomonas Reinhardtii

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Journal J Exp Bot
Specialty Biology
Date 2017 May 19
PMID 28520898
Citations 4
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Abstract

Carbon-concentrating mechanisms (CCMs) enable efficient photosynthesis and growth in CO2-limiting environments, and in eukaryotic microalgae localisation of Rubisco to a microcompartment called the pyrenoid is key. In the model green alga Chlamydomonas reinhardtii, Rubisco preferentially relocalises to the pyrenoid during CCM induction and pyrenoid-less mutants lack a functioning CCM and grow very poorly at low CO2. The aim of this study was to investigate the CO2 response of pyrenoid-positive (pyr+) and pyrenoid-negative (pyr-) mutant strains to determine the effect of pyrenoid absence on CCM induction and gene expression. Shotgun proteomic analysis of low-CO2-adapted strains showed reduced accumulation of some CCM-related proteins, suggesting that pyr- has limited capacity to respond to low-CO2 conditions. Comparisons between gene transcription and protein expression revealed potential regulatory interactions, since Rubisco protein linker (EPYC1) protein did not accumulate in pyr- despite increased transcription, while elements of the LCIB/LCIC complex were also differentially expressed. Furthermore, pyr- showed altered abundance of a number of proteins involved in primary metabolism, perhaps due to the failure to adapt to low CO2. This work highlights two-way regulation between CCM induction and pyrenoid formation, and provides novel candidates for future studies of pyrenoid assembly and CCM function.

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References
1.
Mitchell M, Meyer M, Griffiths H . Dynamics of carbon-concentrating mechanism induction and protein relocalization during the dark-to-light transition in synchronized Chlamydomonas reinhardtii. Plant Physiol. 2014; 166(2):1073-82. PMC: 4213077. DOI: 10.1104/pp.114.246918. View

2.
Kelley L, Sternberg M . Protein structure prediction on the Web: a case study using the Phyre server. Nat Protoc. 2009; 4(3):363-71. DOI: 10.1038/nprot.2009.2. View

3.
KROGH A, Larsson B, von Heijne G, Sonnhammer E . Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol. 2001; 305(3):567-80. DOI: 10.1006/jmbi.2000.4315. View

4.
Wang Y, Spalding M . Acclimation to very low CO2: contribution of limiting CO2 inducible proteins, LCIB and LCIA, to inorganic carbon uptake in Chlamydomonas reinhardtii. Plant Physiol. 2014; 166(4):2040-50. PMC: 4256846. DOI: 10.1104/pp.114.248294. View

5.
Meyer M, Genkov T, Skepper J, Jouhet J, Mitchell M, Spreitzer R . Rubisco small-subunit α-helices control pyrenoid formation in Chlamydomonas. Proc Natl Acad Sci U S A. 2012; 109(47):19474-9. PMC: 3511088. DOI: 10.1073/pnas.1210993109. View