Chlorophyll and Carbohydrate Metabolism in Developing Silique and Seed Are Prerequisite to Seed Oil Content of Brassica Napus L

Overview

Journal Bot Stud

Specialty Biology

Date 2017 May 17

PMID 28510961

Citations 12

Authors

Shuijin Hua

Zhong-Hua Chen

Yaofeng Zhang

Huasheng Yu

Baogang Lin

Dongqing Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Although the seed oil content in canola is a crucial quality determining trait, the regulatory mechanisms of its formation are not fully discovered. This study compared the silique and seed physiological characteristics including fresh and dry weight, seed oil content, chlorophyll content, and carbohydrate content in a high oil content line (HOCL) and a low oil content line (LOCL) of canola derived from a recombinant inbred line in 2010, 2011, and 2012. The aim of the investigation is to uncover the physiological regulation of silique and seed developmental events on seed oil content in canola.

Results: On average, 83% and 86% of silique matter while 69% and 63% of seed matter was produced before 30 days after anthesis (DAA) in HOCL and LOCL, respectively, over three years. Furthermore, HOCL exhibited significantly higher fresh and dry matter at most developmental stages of siliques and seeds. From 20 DAA, lipids were deposited in the seed of HOCL significantly faster than that of LOCL, which was validated by transmission electron microscopy, showing that HOCL accumulates considerable more oil bodies in the seed cells. Markedly higher silique chlorophyll content was observed in HOCL consistently over the three consecutive years, implying a higher potential of photosynthetic capacity in siliques of HOCL. As a consequence, HOCL exhibited significantly higher content of fructose, glucose, sucrose, and starch mainly at 20 to 45 DAA, a key stage of seed lipid deposition. Moreover, seed sugar content was usually higher than silique indicating the importance of sugar transportation from siliques to seeds as substrate for lipid biosynthesis. The much lower silique cellulose content in HOCL was beneficial for lipid synthesis rather than consuming excessive carbohydrate for cell wall.

Conclusions: Superior physiological characteristics of siliques in HOCL showed advantage to produce more photosynthetic assimilates, which were highly correlated to seed oil contents.

Citing Articles

The Dynamic Changes of Seed Microbiota across the Entire Seed Life in the Field.

Yao Y, Liu C, Zhang Y, Lin Y, Chen T, Xie J Plants (Basel). 2024; 13(6).

PMID: 38592934 PMC: 10975644. DOI: 10.3390/plants13060912.

Factors Affecting the Quality of Canola Grains and Their Implications for Grain-Based Foods.

Sabbahi R, Azzaoui K, Rhazi L, Ayerdi-Gotor A, Aussenac T, Depeint F Foods. 2023; 12(11).

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Editorial: Dissection of the molecular basis of fatty acid composition in oil crops and molecular breeding of oil crops with improved fatty acid composition.

Kim H, Li D Front Plant Sci. 2022; 13:1053735.

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Genetic and Biochemical Investigation of Seed Fatty Acid Accumulation in Arabidopsis.

Nwafor C, Li D, Qin P, Li L, Zhang W, Zhou Y Front Plant Sci. 2022; 13:942054.

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Leaf Carbohydrate Metabolism Variation Caused by Late Planting in Rapeseed ( L.) at Reproductive Stage.

Ren Y, Zhu J, Zhang H, Lin B, Hao P, Hua S Plants (Basel). 2022; 11(13).

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