Rhesus Macaque IPSC Generation and Maintenance

Overview

Journal Curr Protoc Stem Cell Biol

Specialty Cell Biology

Date 2017 May 17

PMID 28510330

Citations 3

Authors

Ravi Chandra Yada

So Gun Hong

Yongshun Lin

Thomas Winkler

Cynthia E Dunbar

Affiliations

Soon will be listed here.

Abstract

The rhesus macaque (Macaca mulatta) is physiologically and phylogenetically similar to humans, and therefore represents an invaluable model for the pre-clinical assessment of the safety and feasibility of iPSC-derived cell therapies. The use of an excisable polycistronic lentiviral STEMCCA vector to reprogram rhesus fibroblasts or bone marrow stromal cells (BMSCs) into RhiPSCs is described. After reprogramming, the pluripotency transgenes can be removed by transient expression of Cre, leaving a residual genetic tag that may be useful for identification of RhiPSC-derived tissues in vivo. Finally, the steps to maintain pluripotency during passaging of RhiPSCs, required for successful utilization of RhiPSCs, is described. © 2017 by John Wiley & Sons, Inc.

Citing Articles

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Non-Human Primate iPSC Generation, Cultivation, and Cardiac Differentiation under Chemically Defined Conditions.

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