Biosensor Based on Tyrosinase Immobilized on Graphene-Decorated Gold Nanoparticle/Chitosan for Phenolic Detection in Aqueous

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2017 May 17

PMID 28509848

Citations 11

Authors

Fuzi Mohamed Fartas

Jaafar Abdullah

Nor Azah Yusof

Yusran Sulaiman

Mohd Izham Saiman

Affiliations

Soon will be listed here.

Abstract

In this research work, electrochemical biosensor was fabricated based on immobilization of tyrosinase onto graphene-decorated gold nanoparticle/chitosan (Gr-Au-Chit/Tyr) nanocomposite-modified screen-printed carbon electrode (SPCE) for the detection of phenolic compounds. The nanocomposite film was constructed via solution casting method. The electrocatalytic activity of the proposed biosensor for phenol detection was studied using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Experimental parameters such as pH buffer, enzyme concentration, ratio of Gr-Au-Chit, accumulation time and potential were optimized. The biosensor shows linearity towards phenol in the concentration range from 0.05 to 15 μM with sensitivity of 0.624 μA/μM and the limit of detection (LOD) of 0.016 μM (S/N = 3). The proposed sensor also depicts good reproducibility, selectivity and stability for at least one month. The biosensor was compared with high-performance liquid chromatography (HPLC) method for the detection of phenol spiked in real water samples and the result is in good agreement and comparable.

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References

Vedrine C, Fabiano S, Tran-Minh C . Amperometric tyrosinase based biosensor using an electrogenerated polythiophene film as an entrapment support. Talanta. 2008; 59(3):535-44. View

Mayorga-Martinez C, Cadevall M, Guix M, Ros J, Merkoci A . Bismuth nanoparticles for phenolic compounds biosensing application. Biosens Bioelectron. 2012; 40(1):57-62. DOI: 10.1016/j.bios.2012.06.010. View

Liu Z, Liu B, Kong J, Deng J . Probing trace phenols based on mediator-free alumina sol--gel-derived tyrosinase biosensor. Anal Chem. 2000; 72(19):4707-12. DOI: 10.1021/ac990490h. View

Yu J, Liu S, Ju H . Mediator-free phenol sensor based on titania sol-gel encapsulation matrix for immobilization of tyrosinase by a vapor deposition method. Biosens Bioelectron. 2003; 19(5):509-14. DOI: 10.1016/s0956-5663(03)00227-6. View

Dong X, Huang W, Chen P . In Situ Synthesis of Reduced Graphene Oxide and Gold Nanocomposites for Nanoelectronics and Biosensing. Nanoscale Res Lett. 2016; 6(1):60. PMC: 3212207. DOI: 10.1007/s11671-010-9806-8. View

Chen K, Zhang Z, Liang Y, Liu W . A graphene-based electrochemical sensor for rapid determination of phenols in water. Sensors (Basel). 2013; 13(5):6204-16. PMC: 3690051. DOI: 10.3390/s130506204. View

Wang Z, Zhang J, Yin Z, Wu S, Mandler D, Zhang H . Fabrication of nanoelectrode ensembles by electrodepositon of Au nanoparticles on single-layer graphene oxide sheets. Nanoscale. 2012; 4(8):2728-33. DOI: 10.1039/c2nr30142c. View

Bai R, Muthoosamy K, Zhou M, Ashokkumar M, Huang N, Manickam S . Sonochemical and sustainable synthesis of graphene-gold (G-Au) nanocomposites for enzymeless and selective electrochemical detection of nitric oxide. Biosens Bioelectron. 2016; 87:622-629. DOI: 10.1016/j.bios.2016.09.003. View

Reza K, Ali M, Srivastava S, Agrawal V, Biradar A . Tyrosinase conjugated reduced graphene oxide based biointerface for bisphenol A sensor. Biosens Bioelectron. 2015; 74:644-51. DOI: 10.1016/j.bios.2015.07.020. View

10.

Bin Saiman M, Brett G, Tiruvalam R, Forde M, Sharples K, Thetford A . Involvement of surface-bound radicals in the oxidation of toluene using supported Au-Pd nanoparticles. Angew Chem Int Ed Engl. 2012; 51(24):5981-5. DOI: 10.1002/anie.201201059. View

11.

Lupu S, Lete C, Balaure P, Caval D, Mihailciuc C, Lakard B . Development of amperometric biosensors based on nanostructured tyrosinase-conducting polymer composite electrodes. Sensors (Basel). 2013; 13(5):6759-74. PMC: 3690080. DOI: 10.3390/s130506759. View

12.

Parlak O, Tiwari A, Turner A, Tiwari A . Template-directed hierarchical self-assembly of graphene based hybrid structure for electrochemical biosensing. Biosens Bioelectron. 2013; 49:53-62. DOI: 10.1016/j.bios.2013.04.004. View

13.

Karim M, Lee H . Amperometric phenol biosensor based on covalent immobilization of tyrosinase on Au nanoparticle modified screen printed carbon electrodes. Talanta. 2013; 116:991-6. DOI: 10.1016/j.talanta.2013.08.003. View

14.

Wang S, Tan Y, Zhao D, Liu G . Amperometric tyrosinase biosensor based on Fe3O4 nanoparticles-chitosan nanocomposite. Biosens Bioelectron. 2008; 23(12):1781-7. DOI: 10.1016/j.bios.2008.02.014. View

15.

Lu L, Zhang L, Zhang X, Huan S, Shen G, Yu R . A novel tyrosinase biosensor based on hydroxyapatite-chitosan nanocomposite for the detection of phenolic compounds. Anal Chim Acta. 2010; 665(2):146-51. DOI: 10.1016/j.aca.2010.03.033. View

16.

Rajesh , Bisht V, Takashima W, Kaneto K . An amperometric urea biosensor based on covalent immobilization of urease onto an electrochemically prepared copolymer poly (N-3-aminopropyl pyrrole-co-pyrrole) film. Biomaterials. 2005; 26(17):3683-90. DOI: 10.1016/j.biomaterials.2004.09.024. View

17.

Gu B, Xu C, Zhu G, Liu S, Chen L, Li X . Tyrosinase immobilization on ZnO nanorods for phenol detection. J Phys Chem B. 2008; 113(1):377-81. DOI: 10.1021/jp808001c. View

18.

Yin P, Shah S, Chhowalla M, Lee K . Design, synthesis, and characterization of graphene-nanoparticle hybrid materials for bioapplications. Chem Rev. 2015; 115(7):2483-531. PMC: 5808865. DOI: 10.1021/cr500537t. View

19.

Zhao Z, Zhang M, Chen X, Li Y, Wang J . Electrochemical Co-Reduction Synthesis of AuPt Bimetallic Nanoparticles-Graphene Nanocomposites for Selective Detection of Dopamine in the Presence of Ascorbic Acid and Uric Acid. Sensors (Basel). 2015; 15(7):16614-31. PMC: 4541896. DOI: 10.3390/s150716614. View

20.

Sethuraman V, Muthuraja P, Anandha Raj J, Manisankar P . A highly sensitive electrochemical biosensor for catechol using conducting polymer reduced graphene oxide-metal oxide enzyme modified electrode. Biosens Bioelectron. 2016; 84:112-9. DOI: 10.1016/j.bios.2015.12.074. View